A Whole-Slurry Fermentation Approach to High-Solid Loading for Bioethanol Production from Corn Stover

Corn stover is the most produced byproduct from maize worldwide. Since it is generated as a residue from maize harvesting, it is an inexpensive and interesting crop residue to be used as a feedstock. An ecologically friendly pretreatment such as autohydrolysis was selected for the manufacture of sec...

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Main Authors: Pablo G. del Río, Patricia Gullón, F.R. Rebelo, Aloia Romaní, Gil Garrote, Beatriz Gullón
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:Agronomy
Subjects:
Online Access:https://www.mdpi.com/2073-4395/10/11/1790
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author Pablo G. del Río
Patricia Gullón
F.R. Rebelo
Aloia Romaní
Gil Garrote
Beatriz Gullón
author_facet Pablo G. del Río
Patricia Gullón
F.R. Rebelo
Aloia Romaní
Gil Garrote
Beatriz Gullón
author_sort Pablo G. del Río
collection DOAJ
description Corn stover is the most produced byproduct from maize worldwide. Since it is generated as a residue from maize harvesting, it is an inexpensive and interesting crop residue to be used as a feedstock. An ecologically friendly pretreatment such as autohydrolysis was selected for the manufacture of second-generation bioethanol from corn stover via whole-slurry fermentation at high-solid loadings. Temperatures from 200 to 240 °C were set for the autohydrolysis process, and the solid and liquid phases were analyzed. Additionally, the enzymatic susceptibility of the solid phases was assessed to test the suitability of the pretreatment. Afterward, the production of bioethanol from autohydrolyzed corn stover was carried out, mixing the solid with different percentages of the autohydrolysis liquor (25%, 50%, 75%, and 100%) and water (0% of liquor), from a total whole slurry fermentation (saving energy and water in the liquid–solid separation and subsequent washing of the solid phase) to employing water as only liquid medium. In spite of the challenging scenario of using the liquor fraction as liquid phase in the fermentation, values between 32.2 and 41.9 g ethanol/L and ethanol conversions up to 80% were achieved. This work exhibits the feasibility of corn stover for the production of bioethanol via a whole-slurry fermentation process.
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spelling doaj.art-090fa51e52d84fd9bf70d36a275fca5f2023-11-20T21:03:23ZengMDPI AGAgronomy2073-43952020-11-011011179010.3390/agronomy10111790A Whole-Slurry Fermentation Approach to High-Solid Loading for Bioethanol Production from Corn StoverPablo G. del Río0Patricia Gullón1F.R. Rebelo2Aloia Romaní3Gil Garrote4Beatriz Gullón5Department of Chemical Engineering, Faculty of Science, Universidade de Vigo (Campus Ourense), As Lagoas, 32004 Ourense, SpainNutrition and Bromatology Group, Department of Analytical and Food Chemistry, Faculty of Food Science and Technology, Universidade de Vigo (Campus Ourense), 32004 Ourense, SpainDepartment of Chemical Engineering, Faculty of Science, Universidade de Vigo (Campus Ourense), As Lagoas, 32004 Ourense, SpainDepartment of Chemical Engineering, Faculty of Science, Universidade de Vigo (Campus Ourense), As Lagoas, 32004 Ourense, SpainDepartment of Chemical Engineering, Faculty of Science, Universidade de Vigo (Campus Ourense), As Lagoas, 32004 Ourense, SpainDepartment of Chemical Engineering, Faculty of Science, Universidade de Vigo (Campus Ourense), As Lagoas, 32004 Ourense, SpainCorn stover is the most produced byproduct from maize worldwide. Since it is generated as a residue from maize harvesting, it is an inexpensive and interesting crop residue to be used as a feedstock. An ecologically friendly pretreatment such as autohydrolysis was selected for the manufacture of second-generation bioethanol from corn stover via whole-slurry fermentation at high-solid loadings. Temperatures from 200 to 240 °C were set for the autohydrolysis process, and the solid and liquid phases were analyzed. Additionally, the enzymatic susceptibility of the solid phases was assessed to test the suitability of the pretreatment. Afterward, the production of bioethanol from autohydrolyzed corn stover was carried out, mixing the solid with different percentages of the autohydrolysis liquor (25%, 50%, 75%, and 100%) and water (0% of liquor), from a total whole slurry fermentation (saving energy and water in the liquid–solid separation and subsequent washing of the solid phase) to employing water as only liquid medium. In spite of the challenging scenario of using the liquor fraction as liquid phase in the fermentation, values between 32.2 and 41.9 g ethanol/L and ethanol conversions up to 80% were achieved. This work exhibits the feasibility of corn stover for the production of bioethanol via a whole-slurry fermentation process.https://www.mdpi.com/2073-4395/10/11/1790agro-industrial residueautohydrolysisenvironmentally friendly pretreatmentbiofuelssimultaneous saccharification and fermentation
spellingShingle Pablo G. del Río
Patricia Gullón
F.R. Rebelo
Aloia Romaní
Gil Garrote
Beatriz Gullón
A Whole-Slurry Fermentation Approach to High-Solid Loading for Bioethanol Production from Corn Stover
Agronomy
agro-industrial residue
autohydrolysis
environmentally friendly pretreatment
biofuels
simultaneous saccharification and fermentation
title A Whole-Slurry Fermentation Approach to High-Solid Loading for Bioethanol Production from Corn Stover
title_full A Whole-Slurry Fermentation Approach to High-Solid Loading for Bioethanol Production from Corn Stover
title_fullStr A Whole-Slurry Fermentation Approach to High-Solid Loading for Bioethanol Production from Corn Stover
title_full_unstemmed A Whole-Slurry Fermentation Approach to High-Solid Loading for Bioethanol Production from Corn Stover
title_short A Whole-Slurry Fermentation Approach to High-Solid Loading for Bioethanol Production from Corn Stover
title_sort whole slurry fermentation approach to high solid loading for bioethanol production from corn stover
topic agro-industrial residue
autohydrolysis
environmentally friendly pretreatment
biofuels
simultaneous saccharification and fermentation
url https://www.mdpi.com/2073-4395/10/11/1790
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