Exploring the Possible Impact of Oral Nutritional Supplements on Children’s Oral Health: An In Vitro Investigation
Eight pediatric oral nutritional supplements (ONSs) and 0.5% fat bovine milk were examined in vitro regarding their effect on the adhesion of three caries-related bacteria, <i>Streptococcus mutans</i> (strain CCUG 11877T), <i>Lactobacillus gasseri</i> (strain CCUG 31451), and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Dentistry Journal |
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| Online Access: | https://www.mdpi.com/2304-6767/12/3/78 |
| _version_ | 1797241423638036480 |
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| author | Cynthia Anticona Lena Hansson Ingegerd Johansson Pernilla Lif Holgerson |
| author_facet | Cynthia Anticona Lena Hansson Ingegerd Johansson Pernilla Lif Holgerson |
| author_sort | Cynthia Anticona |
| collection | DOAJ |
| description | Eight pediatric oral nutritional supplements (ONSs) and 0.5% fat bovine milk were examined in vitro regarding their effect on the adhesion of three caries-related bacteria, <i>Streptococcus mutans</i> (strain CCUG 11877T), <i>Lactobacillus gasseri</i> (strain CCUG 31451), and <i>Scardovia wiggsiae</i> (strain CCUG 58090)<i>,</i> to saliva-coated hydroxyapatite, as well as their pH and capacity to withstand pH changes. Bacteria were cultivated and radiolabeled. The adhesion assays used synthetic hydroxyapatite coated with whole or parotid saliva. Measurements of pH and titration of the products with HCl and NaOH were conducted in triplicate. Three ONSs promoted the <i>S. mutans</i> adhesion to saliva-coated hydroxyapatite (increase from 35% to >200%), supporting caries risk enhancement. <i>S. wigssiae</i> and <i>L. gasseri</i> adhered only to one and no ONS, respectively. Most supplements had limited buffering capacity to counteract acidification changes, suggesting their low capacity to neutralize acids, and one ONS showed a significant capacity to counteract basic changes, suggesting a high erosive potential. <i>S. mutans</i> adhesion was influenced by the ONS pH and volume NaOH added to reach pH 10. <i>L. gasseri</i> and <i>S. wiggsiae</i> adhesion was influenced by the ONSs’ carbohydrate and fat content. Interdisciplinary efforts are needed to increase awareness and prevent the possible negative impact of ONSs on children’s oral health. |
| first_indexed | 2024-04-24T18:23:06Z |
| format | Article |
| id | doaj.art-09124d47d25d41bdba2be65a46ea56a1 |
| institution | Directory Open Access Journal |
| issn | 2304-6767 |
| language | English |
| last_indexed | 2024-04-24T18:23:06Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Dentistry Journal |
| spelling | doaj.art-09124d47d25d41bdba2be65a46ea56a12024-03-27T13:33:04ZengMDPI AGDentistry Journal2304-67672024-03-011237810.3390/dj12030078Exploring the Possible Impact of Oral Nutritional Supplements on Children’s Oral Health: An In Vitro InvestigationCynthia Anticona0Lena Hansson1Ingegerd Johansson2Pernilla Lif Holgerson3Department of Odontology, Faculty of Medicine, Umeå University, 90187 Umeå, SwedenDepartment of Clinical Sciences, Pediatrics, Faculty of Medicine, Umeå University, 90187 Umeå, SwedenDepartment of Odontology, Section of Cariology, Faculty of Medicine, Umeå University, 90187 Umeå, SwedenDepartment of Odontology, Section of Pediatric Dentistry, Faculty of Medicine, Umeå University, 90187 Umeå, SwedenEight pediatric oral nutritional supplements (ONSs) and 0.5% fat bovine milk were examined in vitro regarding their effect on the adhesion of three caries-related bacteria, <i>Streptococcus mutans</i> (strain CCUG 11877T), <i>Lactobacillus gasseri</i> (strain CCUG 31451), and <i>Scardovia wiggsiae</i> (strain CCUG 58090)<i>,</i> to saliva-coated hydroxyapatite, as well as their pH and capacity to withstand pH changes. Bacteria were cultivated and radiolabeled. The adhesion assays used synthetic hydroxyapatite coated with whole or parotid saliva. Measurements of pH and titration of the products with HCl and NaOH were conducted in triplicate. Three ONSs promoted the <i>S. mutans</i> adhesion to saliva-coated hydroxyapatite (increase from 35% to >200%), supporting caries risk enhancement. <i>S. wigssiae</i> and <i>L. gasseri</i> adhered only to one and no ONS, respectively. Most supplements had limited buffering capacity to counteract acidification changes, suggesting their low capacity to neutralize acids, and one ONS showed a significant capacity to counteract basic changes, suggesting a high erosive potential. <i>S. mutans</i> adhesion was influenced by the ONS pH and volume NaOH added to reach pH 10. <i>L. gasseri</i> and <i>S. wiggsiae</i> adhesion was influenced by the ONSs’ carbohydrate and fat content. Interdisciplinary efforts are needed to increase awareness and prevent the possible negative impact of ONSs on children’s oral health.https://www.mdpi.com/2304-6767/12/3/78cariogenic potentialdental erosive potentialoral healthpediatric oral nutritional supplements |
| spellingShingle | Cynthia Anticona Lena Hansson Ingegerd Johansson Pernilla Lif Holgerson Exploring the Possible Impact of Oral Nutritional Supplements on Children’s Oral Health: An In Vitro Investigation Dentistry Journal cariogenic potential dental erosive potential oral health pediatric oral nutritional supplements |
| title | Exploring the Possible Impact of Oral Nutritional Supplements on Children’s Oral Health: An In Vitro Investigation |
| title_full | Exploring the Possible Impact of Oral Nutritional Supplements on Children’s Oral Health: An In Vitro Investigation |
| title_fullStr | Exploring the Possible Impact of Oral Nutritional Supplements on Children’s Oral Health: An In Vitro Investigation |
| title_full_unstemmed | Exploring the Possible Impact of Oral Nutritional Supplements on Children’s Oral Health: An In Vitro Investigation |
| title_short | Exploring the Possible Impact of Oral Nutritional Supplements on Children’s Oral Health: An In Vitro Investigation |
| title_sort | exploring the possible impact of oral nutritional supplements on children s oral health an in vitro investigation |
| topic | cariogenic potential dental erosive potential oral health pediatric oral nutritional supplements |
| url | https://www.mdpi.com/2304-6767/12/3/78 |
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