Marker-Independent Monitoring of in vitro and in vivo Degradation of Supramolecular Polymers Applied in Cardiovascular in situ Tissue Engineering
The equilibrium between scaffold degradation and neotissue formation, is highly essential for in situ tissue engineering. Herein, biodegradable grafts function as temporal roadmap to guide regeneration. The ability to monitor and understand the dynamics of degradation and tissue deposition in in sit...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Cardiovascular Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2022.885873/full |
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| author | Julia Marzi Julia Marzi Julia Marzi Emma C. Munnig Schmidt Eva M. Brauchle Eva M. Brauchle Eva M. Brauchle Tamar B. Wissing Tamar B. Wissing Hannah Bauer Aurelie Serrero Serge H. M. Söntjens Anton W. Bosman Martijn A. J. Cox Anthal I. P. M. Smits Anthal I. P. M. Smits Katja Schenke-Layland Katja Schenke-Layland Katja Schenke-Layland Katja Schenke-Layland |
| author_facet | Julia Marzi Julia Marzi Julia Marzi Emma C. Munnig Schmidt Eva M. Brauchle Eva M. Brauchle Eva M. Brauchle Tamar B. Wissing Tamar B. Wissing Hannah Bauer Aurelie Serrero Serge H. M. Söntjens Anton W. Bosman Martijn A. J. Cox Anthal I. P. M. Smits Anthal I. P. M. Smits Katja Schenke-Layland Katja Schenke-Layland Katja Schenke-Layland Katja Schenke-Layland |
| author_sort | Julia Marzi |
| collection | DOAJ |
| description | The equilibrium between scaffold degradation and neotissue formation, is highly essential for in situ tissue engineering. Herein, biodegradable grafts function as temporal roadmap to guide regeneration. The ability to monitor and understand the dynamics of degradation and tissue deposition in in situ cardiovascular graft materials is therefore of great value to accelerate the implementation of safe and sustainable tissue-engineered vascular grafts (TEVGs) as a substitute for conventional prosthetic grafts. In this study, we investigated the potential of Raman microspectroscopy and Raman imaging to monitor degradation kinetics of supramolecular polymers, which are employed as degradable scaffolds in in situ tissue engineering. Raman imaging was applied on in vitro degraded polymers, investigating two different polymer materials, subjected to oxidative and enzymatically-induced degradation. Furthermore, the method was transferred to analyze in vivo degradation of tissue-engineered carotid grafts after 6 and 12 months in a sheep model. Multivariate data analysis allowed to trace degradation and to compare the data from in vitro and in vivo degradation, indicating similar molecular observations in spectral signatures between implants and oxidative in vitro degradation. In vivo degradation appeared to be dominated by oxidative pathways. Furthermore, information on collagen deposition and composition could simultaneously be obtained from the same image scans. Our results demonstrate the sensitivity of Raman microspectroscopy to determine degradation stages and the assigned molecular changes non-destructively, encouraging future exploration of this techniques for time-resolved quality assessment of in situ tissue engineering processes. |
| first_indexed | 2024-04-12T11:48:59Z |
| format | Article |
| id | doaj.art-09126f20f0e34bd79e1822c8f938f2c5 |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-04-12T11:48:59Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-09126f20f0e34bd79e1822c8f938f2c52022-12-22T03:34:15ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2022-05-01910.3389/fcvm.2022.885873885873Marker-Independent Monitoring of in vitro and in vivo Degradation of Supramolecular Polymers Applied in Cardiovascular in situ Tissue EngineeringJulia Marzi0Julia Marzi1Julia Marzi2Emma C. Munnig Schmidt3Eva M. Brauchle4Eva M. Brauchle5Eva M. Brauchle6Tamar B. Wissing7Tamar B. Wissing8Hannah Bauer9Aurelie Serrero10Serge H. M. Söntjens11Anton W. Bosman12Martijn A. J. Cox13Anthal I. P. M. Smits14Anthal I. P. M. Smits15Katja Schenke-Layland16Katja Schenke-Layland17Katja Schenke-Layland18Katja Schenke-Layland19Department for Medical Technologies and Regenerative Medicine, Institute of Biomedical Engineering, Eberhard Karls University Tübingen, Tübingen, GermanyNMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, GermanyCluster of Excellence iFIT (EXC 2180) “Image-Guided and Functionally Instructed Tumor Therapies, ” Eberhard Karls University Tübingen, Tübingen, GermanyDepartment of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, NetherlandsDepartment for Medical Technologies and Regenerative Medicine, Institute of Biomedical Engineering, Eberhard Karls University Tübingen, Tübingen, GermanyNMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, GermanyCluster of Excellence iFIT (EXC 2180) “Image-Guided and Functionally Instructed Tumor Therapies, ” Eberhard Karls University Tübingen, Tübingen, GermanyDepartment of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, NetherlandsInstitute for Complex Molecular Systems (ICMS), Eindhoven University of TechnologyEindhoven, NetherlandsXeltis BV, Eindhoven, NetherlandsXeltis BV, Eindhoven, NetherlandsSyMO-Chem BV, Eindhoven, NetherlandsSupraPolix BV, Eindhoven, NetherlandsXeltis BV, Eindhoven, NetherlandsDepartment of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, NetherlandsInstitute for Complex Molecular Systems (ICMS), Eindhoven University of TechnologyEindhoven, NetherlandsDepartment for Medical Technologies and Regenerative Medicine, Institute of Biomedical Engineering, Eberhard Karls University Tübingen, Tübingen, GermanyNMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, GermanyCluster of Excellence iFIT (EXC 2180) “Image-Guided and Functionally Instructed Tumor Therapies, ” Eberhard Karls University Tübingen, Tübingen, GermanyCardiovascular Research Laboratories, Department of Medicine, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA, United StatesThe equilibrium between scaffold degradation and neotissue formation, is highly essential for in situ tissue engineering. Herein, biodegradable grafts function as temporal roadmap to guide regeneration. The ability to monitor and understand the dynamics of degradation and tissue deposition in in situ cardiovascular graft materials is therefore of great value to accelerate the implementation of safe and sustainable tissue-engineered vascular grafts (TEVGs) as a substitute for conventional prosthetic grafts. In this study, we investigated the potential of Raman microspectroscopy and Raman imaging to monitor degradation kinetics of supramolecular polymers, which are employed as degradable scaffolds in in situ tissue engineering. Raman imaging was applied on in vitro degraded polymers, investigating two different polymer materials, subjected to oxidative and enzymatically-induced degradation. Furthermore, the method was transferred to analyze in vivo degradation of tissue-engineered carotid grafts after 6 and 12 months in a sheep model. Multivariate data analysis allowed to trace degradation and to compare the data from in vitro and in vivo degradation, indicating similar molecular observations in spectral signatures between implants and oxidative in vitro degradation. In vivo degradation appeared to be dominated by oxidative pathways. Furthermore, information on collagen deposition and composition could simultaneously be obtained from the same image scans. Our results demonstrate the sensitivity of Raman microspectroscopy to determine degradation stages and the assigned molecular changes non-destructively, encouraging future exploration of this techniques for time-resolved quality assessment of in situ tissue engineering processes.https://www.frontiersin.org/articles/10.3389/fcvm.2022.885873/fullRaman imagingcarotid implantationtissue-engineered vascular grafts (TEVG)guided tissue engineeringresorption |
| spellingShingle | Julia Marzi Julia Marzi Julia Marzi Emma C. Munnig Schmidt Eva M. Brauchle Eva M. Brauchle Eva M. Brauchle Tamar B. Wissing Tamar B. Wissing Hannah Bauer Aurelie Serrero Serge H. M. Söntjens Anton W. Bosman Martijn A. J. Cox Anthal I. P. M. Smits Anthal I. P. M. Smits Katja Schenke-Layland Katja Schenke-Layland Katja Schenke-Layland Katja Schenke-Layland Marker-Independent Monitoring of in vitro and in vivo Degradation of Supramolecular Polymers Applied in Cardiovascular in situ Tissue Engineering Frontiers in Cardiovascular Medicine Raman imaging carotid implantation tissue-engineered vascular grafts (TEVG) guided tissue engineering resorption |
| title | Marker-Independent Monitoring of in vitro and in vivo Degradation of Supramolecular Polymers Applied in Cardiovascular in situ Tissue Engineering |
| title_full | Marker-Independent Monitoring of in vitro and in vivo Degradation of Supramolecular Polymers Applied in Cardiovascular in situ Tissue Engineering |
| title_fullStr | Marker-Independent Monitoring of in vitro and in vivo Degradation of Supramolecular Polymers Applied in Cardiovascular in situ Tissue Engineering |
| title_full_unstemmed | Marker-Independent Monitoring of in vitro and in vivo Degradation of Supramolecular Polymers Applied in Cardiovascular in situ Tissue Engineering |
| title_short | Marker-Independent Monitoring of in vitro and in vivo Degradation of Supramolecular Polymers Applied in Cardiovascular in situ Tissue Engineering |
| title_sort | marker independent monitoring of in vitro and in vivo degradation of supramolecular polymers applied in cardiovascular in situ tissue engineering |
| topic | Raman imaging carotid implantation tissue-engineered vascular grafts (TEVG) guided tissue engineering resorption |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2022.885873/full |
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