Monospecies probiotic preparation and administration with downstream analysis of sex-specific effects on gut microbiome composition in mice
Summary: Dysbiosis of the gut microbiome is implicated in the growing burden of non-communicable chronic diseases, including neurodevelopmental disorders, and both preclinical and clinical studies highlight the potential for precision probiotic therapies in their prevention and treatment. Here, we p...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723003532 |
| _version_ | 1797792567914397696 |
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| author | Claudia M. Di Gesù Lisa M. Matz Robert Fultz Ian J. Bolding Shelly A. Buffington |
| author_facet | Claudia M. Di Gesù Lisa M. Matz Robert Fultz Ian J. Bolding Shelly A. Buffington |
| author_sort | Claudia M. Di Gesù |
| collection | DOAJ |
| description | Summary: Dysbiosis of the gut microbiome is implicated in the growing burden of non-communicable chronic diseases, including neurodevelopmental disorders, and both preclinical and clinical studies highlight the potential for precision probiotic therapies in their prevention and treatment. Here, we present an optimized protocol for the preparation and administration of Limosilactobacillus reuteri MM4-1A (ATCC-PTA-6475) to adolescent mice. We also describe steps for performing downstream analysis of metataxonomic sequencing data with careful assessment of sex-specific effects on microbiome composition and structure.For complete details on the use and execution of this protocol, please refer to Di Gesù et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-13T02:35:54Z |
| format | Article |
| id | doaj.art-0913e4659ecf45e0b9016572bdade25d |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-13T02:35:54Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-0913e4659ecf45e0b9016572bdade25d2023-06-29T04:14:34ZengElsevierSTAR Protocols2666-16672023-09-0143102386Monospecies probiotic preparation and administration with downstream analysis of sex-specific effects on gut microbiome composition in miceClaudia M. Di Gesù0Lisa M. Matz1Robert Fultz2Ian J. Bolding3Shelly A. Buffington4Department of Neurobiology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555, USA; Department of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, ItalyDepartment of Neurobiology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555, USADepartment of Neurobiology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555, USADepartment of Neurobiology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555, USADepartment of Neurobiology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555, USA; Sealy Center for Microbiome Research, The University of Texas Medical Branch, Galveston, TX 77555, USA; Corresponding authorSummary: Dysbiosis of the gut microbiome is implicated in the growing burden of non-communicable chronic diseases, including neurodevelopmental disorders, and both preclinical and clinical studies highlight the potential for precision probiotic therapies in their prevention and treatment. Here, we present an optimized protocol for the preparation and administration of Limosilactobacillus reuteri MM4-1A (ATCC-PTA-6475) to adolescent mice. We also describe steps for performing downstream analysis of metataxonomic sequencing data with careful assessment of sex-specific effects on microbiome composition and structure.For complete details on the use and execution of this protocol, please refer to Di Gesù et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723003532BioinformaticsMicrobiologyNeuroscienceSequence analysis |
| spellingShingle | Claudia M. Di Gesù Lisa M. Matz Robert Fultz Ian J. Bolding Shelly A. Buffington Monospecies probiotic preparation and administration with downstream analysis of sex-specific effects on gut microbiome composition in mice STAR Protocols Bioinformatics Microbiology Neuroscience Sequence analysis |
| title | Monospecies probiotic preparation and administration with downstream analysis of sex-specific effects on gut microbiome composition in mice |
| title_full | Monospecies probiotic preparation and administration with downstream analysis of sex-specific effects on gut microbiome composition in mice |
| title_fullStr | Monospecies probiotic preparation and administration with downstream analysis of sex-specific effects on gut microbiome composition in mice |
| title_full_unstemmed | Monospecies probiotic preparation and administration with downstream analysis of sex-specific effects on gut microbiome composition in mice |
| title_short | Monospecies probiotic preparation and administration with downstream analysis of sex-specific effects on gut microbiome composition in mice |
| title_sort | monospecies probiotic preparation and administration with downstream analysis of sex specific effects on gut microbiome composition in mice |
| topic | Bioinformatics Microbiology Neuroscience Sequence analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723003532 |
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