Antibacterial Activity against Clinical Strains of a Natural Polyphenolic Extract from Albariño White Grape Marc
Infections caused by multidrug-resistant bacteria are becoming increasingly frequent and sometimes difficult to treat due to the limited number of antibiotics active against them. In addition, they can spread between countries and/or continents, which is a problem of great relevance worldwide. It is...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/16/7/950 |
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| author | Tamara Manso Marta Lores José Luis R. Rama Rosa-Antía Villarino Lorena G. Calvo Aly Castillo María Celeiro Trinidad de Miguel |
| author_facet | Tamara Manso Marta Lores José Luis R. Rama Rosa-Antía Villarino Lorena G. Calvo Aly Castillo María Celeiro Trinidad de Miguel |
| author_sort | Tamara Manso |
| collection | DOAJ |
| description | Infections caused by multidrug-resistant bacteria are becoming increasingly frequent and sometimes difficult to treat due to the limited number of antibiotics active against them. In addition, they can spread between countries and/or continents, which is a problem of great relevance worldwide. It is, therefore, urgent to find alternatives to treat infections caused by multidrug-resistant bacteria. This study aimed at exploring a possible therapeutic alternative in the fight against antibiotic resistance. Based on the known antibacterial capacity of polyphenols, we tested the antimicrobial activity of a polyphenolic extract of Albariño white grape marc on clinical strains since research on such bacteria has been very scarce until now. First, the extract was obtained using a medium-scale ambient temperature (MSAT) system, which is an efficient and sustainable extractive method. The determinations of the polyphenolic content of the extract and its antioxidant capacity showed good results. Using chromatographic and mass spectrometric tools, 13 remarkable polyphenols were detected in the extract. The antibacterial activity of our grape marc extract against nineteen clinical strain isolates, some of which are multidrug-resistant, was evaluated by means of the calculation of half of the maximum inhibitory concentration (IC50) and the value of the minimum bactericidal concentrations (MBCs). In conclusion, the extract showed effectiveness against all clinical strains tested, regardless of their level of antibiotic resistance, and shows promise in the fight against antibiotic resistance. |
| first_indexed | 2024-03-11T00:44:33Z |
| format | Article |
| id | doaj.art-0914ed45b9c24f33a14809d34941ca83 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-11T00:44:33Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-0914ed45b9c24f33a14809d34941ca832023-11-18T20:52:15ZengMDPI AGPharmaceuticals1424-82472023-07-0116795010.3390/ph16070950Antibacterial Activity against Clinical Strains of a Natural Polyphenolic Extract from Albariño White Grape MarcTamara Manso0Marta Lores1José Luis R. Rama2Rosa-Antía Villarino3Lorena G. Calvo4Aly Castillo5María Celeiro6Trinidad de Miguel7Complejo Hospitalario Universitario de Ferrol, E-15405 Ferrol, SpainLaboratory of Research and Development of Analytical Solutions (LIDSA), Department of Analytical Chemistry, Nutrition and Food Science, Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, SpainDepartment of Microbiology and Parasitology, Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, SpainDepartment of Microbiology and Parasitology, Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, SpainDepartment of Microbiology and Parasitology, Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, SpainLaboratory of Research and Development of Analytical Solutions (LIDSA), Department of Analytical Chemistry, Nutrition and Food Science, Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, SpainLaboratory of Research and Development of Analytical Solutions (LIDSA), Department of Analytical Chemistry, Nutrition and Food Science, Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, SpainDepartment of Microbiology and Parasitology, Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, SpainInfections caused by multidrug-resistant bacteria are becoming increasingly frequent and sometimes difficult to treat due to the limited number of antibiotics active against them. In addition, they can spread between countries and/or continents, which is a problem of great relevance worldwide. It is, therefore, urgent to find alternatives to treat infections caused by multidrug-resistant bacteria. This study aimed at exploring a possible therapeutic alternative in the fight against antibiotic resistance. Based on the known antibacterial capacity of polyphenols, we tested the antimicrobial activity of a polyphenolic extract of Albariño white grape marc on clinical strains since research on such bacteria has been very scarce until now. First, the extract was obtained using a medium-scale ambient temperature (MSAT) system, which is an efficient and sustainable extractive method. The determinations of the polyphenolic content of the extract and its antioxidant capacity showed good results. Using chromatographic and mass spectrometric tools, 13 remarkable polyphenols were detected in the extract. The antibacterial activity of our grape marc extract against nineteen clinical strain isolates, some of which are multidrug-resistant, was evaluated by means of the calculation of half of the maximum inhibitory concentration (IC50) and the value of the minimum bactericidal concentrations (MBCs). In conclusion, the extract showed effectiveness against all clinical strains tested, regardless of their level of antibiotic resistance, and shows promise in the fight against antibiotic resistance.https://www.mdpi.com/1424-8247/16/7/950antibacterial activitynatural extractwhite grape marcpolyphenolsclinical strainantimicrobial resistance |
| spellingShingle | Tamara Manso Marta Lores José Luis R. Rama Rosa-Antía Villarino Lorena G. Calvo Aly Castillo María Celeiro Trinidad de Miguel Antibacterial Activity against Clinical Strains of a Natural Polyphenolic Extract from Albariño White Grape Marc Pharmaceuticals antibacterial activity natural extract white grape marc polyphenols clinical strain antimicrobial resistance |
| title | Antibacterial Activity against Clinical Strains of a Natural Polyphenolic Extract from Albariño White Grape Marc |
| title_full | Antibacterial Activity against Clinical Strains of a Natural Polyphenolic Extract from Albariño White Grape Marc |
| title_fullStr | Antibacterial Activity against Clinical Strains of a Natural Polyphenolic Extract from Albariño White Grape Marc |
| title_full_unstemmed | Antibacterial Activity against Clinical Strains of a Natural Polyphenolic Extract from Albariño White Grape Marc |
| title_short | Antibacterial Activity against Clinical Strains of a Natural Polyphenolic Extract from Albariño White Grape Marc |
| title_sort | antibacterial activity against clinical strains of a natural polyphenolic extract from albarino white grape marc |
| topic | antibacterial activity natural extract white grape marc polyphenols clinical strain antimicrobial resistance |
| url | https://www.mdpi.com/1424-8247/16/7/950 |
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