MOLECULAR-BIOLOGICAL MARKERS OF HEPATITIS В IN PATIENTS WITH LIVER FIBROSIS/CIRRHOSIS IN UZBEKISTAN
Aim. Evaluate prevalence of genetic variants of hepatitis В viruses in population of various regions of Uzbekistan with hepatitis of various genesis and different severity levels of liver fibrosis and cirrhosis. Materials and methods. Blood plasma and liver biopsy from 39 patients with different sev...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2016-10-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.elpub.ru/jour/article/view/85 |
| _version_ | 1818081053674307584 |
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| author | Yu. V. Ostankova A. V. Semenov Kh. N. Faizullaev E. I. Kazakova A. V. Kozlov E. I. Musabaev A. A. Totolyan |
| author_facet | Yu. V. Ostankova A. V. Semenov Kh. N. Faizullaev E. I. Kazakova A. V. Kozlov E. I. Musabaev A. A. Totolyan |
| author_sort | Yu. V. Ostankova |
| collection | DOAJ |
| description | Aim. Evaluate prevalence of genetic variants of hepatitis В viruses in population of various regions of Uzbekistan with hepatitis of various genesis and different severity levels of liver fibrosis and cirrhosis. Materials and methods. Blood plasma and liver biopsy from 39 patients with different severity levels of liver fibrosis and cirrhosis served as study material. Genotyping based on direct sequencing of Pre-Sl/Pre-S2/S HBV DNA region was applied. Results. Hepatitis В virus was detected in 32 samples ofthe 39 provided, frequency of occurrence - 82%, respectively. Phylogenetic analysis has shown, that only genotype D was detected among the examined patients, hepatitis В virus subtype D1 predominated (84.38%) compared with D2 (3.12%) and D3 (12.5%) subtypes. Conclusion. Large-scale sequencing of HBV in Central Asia will allow to evaluate routes of transmission and time of evolutionary separation of virus isolates. Understanding the epidemiology of the infectious process is important for development of programs for prophylaxis and therapy of the infection. |
| first_indexed | 2024-12-10T19:00:06Z |
| format | Article |
| id | doaj.art-091b236ad4864b018b10e1a9d5c3c116 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-12-10T19:00:06Z |
| publishDate | 2016-10-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-091b236ad4864b018b10e1a9d5c3c1162022-12-22T01:37:01ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132016-10-0105344310.36233/0372-9311-2016-5-34-4385MOLECULAR-BIOLOGICAL MARKERS OF HEPATITIS В IN PATIENTS WITH LIVER FIBROSIS/CIRRHOSIS IN UZBEKISTANYu. V. Ostankova0A. V. Semenov1Kh. N. Faizullaev2E. I. Kazakova3A. V. Kozlov4E. I. Musabaev5A. A. Totolyan6Pasteur Research Institute of Epidemiology and MicrobiologyPasteur Research Institute of Epidemiology and Microbiology; Pavlov State Medical University; Mechnikov North-Western State Medical UniversityResearch Institute of VirologyResearch Institute of VirologyMechnikov North-Western State Medical UniversityResearch Institute of VirologyPasteur Research Institute of Epidemiology and Microbiology; Pavlov State Medical UniversityAim. Evaluate prevalence of genetic variants of hepatitis В viruses in population of various regions of Uzbekistan with hepatitis of various genesis and different severity levels of liver fibrosis and cirrhosis. Materials and methods. Blood plasma and liver biopsy from 39 patients with different severity levels of liver fibrosis and cirrhosis served as study material. Genotyping based on direct sequencing of Pre-Sl/Pre-S2/S HBV DNA region was applied. Results. Hepatitis В virus was detected in 32 samples ofthe 39 provided, frequency of occurrence - 82%, respectively. Phylogenetic analysis has shown, that only genotype D was detected among the examined patients, hepatitis В virus subtype D1 predominated (84.38%) compared with D2 (3.12%) and D3 (12.5%) subtypes. Conclusion. Large-scale sequencing of HBV in Central Asia will allow to evaluate routes of transmission and time of evolutionary separation of virus isolates. Understanding the epidemiology of the infectious process is important for development of programs for prophylaxis and therapy of the infection.https://microbiol.elpub.ru/jour/article/view/85hepatitis bdeep genotypingsequencingmolecular epidemiologyuzbekistan |
| spellingShingle | Yu. V. Ostankova A. V. Semenov Kh. N. Faizullaev E. I. Kazakova A. V. Kozlov E. I. Musabaev A. A. Totolyan MOLECULAR-BIOLOGICAL MARKERS OF HEPATITIS В IN PATIENTS WITH LIVER FIBROSIS/CIRRHOSIS IN UZBEKISTAN Журнал микробиологии, эпидемиологии и иммунобиологии hepatitis b deep genotyping sequencing molecular epidemiology uzbekistan |
| title | MOLECULAR-BIOLOGICAL MARKERS OF HEPATITIS В IN PATIENTS WITH LIVER FIBROSIS/CIRRHOSIS IN UZBEKISTAN |
| title_full | MOLECULAR-BIOLOGICAL MARKERS OF HEPATITIS В IN PATIENTS WITH LIVER FIBROSIS/CIRRHOSIS IN UZBEKISTAN |
| title_fullStr | MOLECULAR-BIOLOGICAL MARKERS OF HEPATITIS В IN PATIENTS WITH LIVER FIBROSIS/CIRRHOSIS IN UZBEKISTAN |
| title_full_unstemmed | MOLECULAR-BIOLOGICAL MARKERS OF HEPATITIS В IN PATIENTS WITH LIVER FIBROSIS/CIRRHOSIS IN UZBEKISTAN |
| title_short | MOLECULAR-BIOLOGICAL MARKERS OF HEPATITIS В IN PATIENTS WITH LIVER FIBROSIS/CIRRHOSIS IN UZBEKISTAN |
| title_sort | molecular biological markers of hepatitis в in patients with liver fibrosis cirrhosis in uzbekistan |
| topic | hepatitis b deep genotyping sequencing molecular epidemiology uzbekistan |
| url | https://microbiol.elpub.ru/jour/article/view/85 |
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