New and Old Genes Associated with Primary and Established Responses to Paclitaxel Treatment in Ovarian Cancer Cell Lines
Development of drug resistance is the main reason for low chemotherapy effectiveness in treating ovarian cancer. Paclitaxel (PAC) is a chemotherapeutic drug used in the treatment of this cancer. We analysed the development of PAC resistance in two ovarian cancer cell lines. Exposure of drug-sensitiv...
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MDPI AG
2018-04-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/4/891 |
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| author | Monika Świerczewska Andrzej Klejewski Maciej Brązert Dominika Kaźmierczak Dariusz Iżycki Michał Nowicki Maciej Zabel Radosław Januchowski |
| author_facet | Monika Świerczewska Andrzej Klejewski Maciej Brązert Dominika Kaźmierczak Dariusz Iżycki Michał Nowicki Maciej Zabel Radosław Januchowski |
| author_sort | Monika Świerczewska |
| collection | DOAJ |
| description | Development of drug resistance is the main reason for low chemotherapy effectiveness in treating ovarian cancer. Paclitaxel (PAC) is a chemotherapeutic drug used in the treatment of this cancer. We analysed the development of PAC resistance in two ovarian cancer cell lines. Exposure of drug-sensitive cell lines (A2780 and W1) to PAC was used to determine the primary response. An established response was determined in PAC-resistant sublines of the A2780 and W1 cell lines. qRT-PCR was performed to measure the expression levels of specific genes. We observed decreased expression of the PCDH9, NSBP1, MCTP1 and SEMA3A genes in the PAC-resistant cell lines. Short-term exposure to PAC led to increased expression of the MDR1 and BCRP genes in the A2780 and W1 cell lines. In the A2780 cell line, we also observed increased expression of the C4orf18 gene and decreased expression of the PCDH9 and SEMA3A genes after PAC treatment. In the W1 cell line, short-term treatment with PAC upregulated the expression of the ALDH1A1 gene, a marker of Cancer stem cells (CSCs). Our results suggest that downregulation of the PCDH9, NSBP1, MCTP1 and SEMA3A genes and upregulation of the MDR1, BCRP, C4orf18 and ALDH1A1 genes may be related to PAC resistance. |
| first_indexed | 2024-12-11T01:13:19Z |
| format | Article |
| id | doaj.art-091c4353bf7b43dab6d72c0bc62acafb |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T01:13:19Z |
| publishDate | 2018-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-091c4353bf7b43dab6d72c0bc62acafb2022-12-22T01:25:57ZengMDPI AGMolecules1420-30492018-04-0123489110.3390/molecules23040891molecules23040891New and Old Genes Associated with Primary and Established Responses to Paclitaxel Treatment in Ovarian Cancer Cell LinesMonika Świerczewska0Andrzej Klejewski1Maciej Brązert2Dominika Kaźmierczak3Dariusz Iżycki4Michał Nowicki5Maciej Zabel6Radosław Januchowski7Department of Histology and Embryology, Poznan University of Medical Sciences, Święcickiego 6 St., 61-781 Poznań, PolandDepartment of Nursing, Poznan University of Medical Sciences, Smoluchowskiego 11 St., 60-179 Poznan, PolandDivision of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, Polna 33 St., 60-535 Poznań, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, Święcickiego 6 St., 61-781 Poznań, PolandDepartment of Cancer Immunology, Poznan University of Medical Sciences, Garbary 15 St., 61-866 Poznań, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, Święcickiego 6 St., 61-781 Poznań, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, Święcickiego 6 St., 61-781 Poznań, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, Święcickiego 6 St., 61-781 Poznań, PolandDevelopment of drug resistance is the main reason for low chemotherapy effectiveness in treating ovarian cancer. Paclitaxel (PAC) is a chemotherapeutic drug used in the treatment of this cancer. We analysed the development of PAC resistance in two ovarian cancer cell lines. Exposure of drug-sensitive cell lines (A2780 and W1) to PAC was used to determine the primary response. An established response was determined in PAC-resistant sublines of the A2780 and W1 cell lines. qRT-PCR was performed to measure the expression levels of specific genes. We observed decreased expression of the PCDH9, NSBP1, MCTP1 and SEMA3A genes in the PAC-resistant cell lines. Short-term exposure to PAC led to increased expression of the MDR1 and BCRP genes in the A2780 and W1 cell lines. In the A2780 cell line, we also observed increased expression of the C4orf18 gene and decreased expression of the PCDH9 and SEMA3A genes after PAC treatment. In the W1 cell line, short-term treatment with PAC upregulated the expression of the ALDH1A1 gene, a marker of Cancer stem cells (CSCs). Our results suggest that downregulation of the PCDH9, NSBP1, MCTP1 and SEMA3A genes and upregulation of the MDR1, BCRP, C4orf18 and ALDH1A1 genes may be related to PAC resistance.http://www.mdpi.com/1420-3049/23/4/891ovarian cancerpaclitaxel resistancenew genes |
| spellingShingle | Monika Świerczewska Andrzej Klejewski Maciej Brązert Dominika Kaźmierczak Dariusz Iżycki Michał Nowicki Maciej Zabel Radosław Januchowski New and Old Genes Associated with Primary and Established Responses to Paclitaxel Treatment in Ovarian Cancer Cell Lines Molecules ovarian cancer paclitaxel resistance new genes |
| title | New and Old Genes Associated with Primary and Established Responses to Paclitaxel Treatment in Ovarian Cancer Cell Lines |
| title_full | New and Old Genes Associated with Primary and Established Responses to Paclitaxel Treatment in Ovarian Cancer Cell Lines |
| title_fullStr | New and Old Genes Associated with Primary and Established Responses to Paclitaxel Treatment in Ovarian Cancer Cell Lines |
| title_full_unstemmed | New and Old Genes Associated with Primary and Established Responses to Paclitaxel Treatment in Ovarian Cancer Cell Lines |
| title_short | New and Old Genes Associated with Primary and Established Responses to Paclitaxel Treatment in Ovarian Cancer Cell Lines |
| title_sort | new and old genes associated with primary and established responses to paclitaxel treatment in ovarian cancer cell lines |
| topic | ovarian cancer paclitaxel resistance new genes |
| url | http://www.mdpi.com/1420-3049/23/4/891 |
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