Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8
Keratinocyte line cells HaCaT and FEPE1L-8 are used for skin model with type I collagen fibrils (gels). For this purpose, not only differentiation but also regulation of proliferation on type I collagen gels by exogenous calcium concentration is important. When exogenous calcium concentration is low...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-06-01
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| Series: | Regenerative Therapy |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320417300408 |
| _version_ | 1818881829390778368 |
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| author | Hitomi Fujisaki Sugiko Futaki Masashi Yamada Kiyotoshi Sekiguchi Toshihiko Hayashi Takashi Ikejima Shunji Hattori |
| author_facet | Hitomi Fujisaki Sugiko Futaki Masashi Yamada Kiyotoshi Sekiguchi Toshihiko Hayashi Takashi Ikejima Shunji Hattori |
| author_sort | Hitomi Fujisaki |
| collection | DOAJ |
| description | Keratinocyte line cells HaCaT and FEPE1L-8 are used for skin model with type I collagen fibrils (gels). For this purpose, not only differentiation but also regulation of proliferation on type I collagen gels by exogenous calcium concentration is important. When exogenous calcium concentration is low, primary keratinocyte proliferation is repressed and eventually cells are induced to apoptosis on type I collagen gels. The apoptosis induced on type I collagen gels is suppressed by increasing calcium concentration in the medium. That is, higher exogenous calcium concentration is necessary for primary keratinocyte survival on type I collagen gels than for that on dish surface culture. Meanwhile much higher exogenous calcium causes cell differentiation and inhibition of proliferation. The optimal calcium concentrations for proliferation on type I collagen gels have not been clarified in keratinocyte line cells. HaCaT cells have a unique calcium sensitivity in comparison with primary keratinocytes, whereas FEPE1L-8 cells have a similar sensitivity to primary keratinocytes. In this study, we compared the effect of calcium concentrations on proliferation of HaCaT and FEPE1L-8 cells on type I collagen gels. On type I collagen gels, both line cells required higher calcium concentrations for proliferation than on dish surface. HaCaT cells proliferated better in a wider range of calcium concentrations than FEPE1L-8 cells. Keywords: Type I collagen gel, Keratinocyte proliferation, Calcium concentration |
| first_indexed | 2024-12-19T15:08:05Z |
| format | Article |
| id | doaj.art-091ce8d239304a0aaadc173be72f0bdf |
| institution | Directory Open Access Journal |
| issn | 2352-3204 |
| language | English |
| last_indexed | 2024-12-19T15:08:05Z |
| publishDate | 2018-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj.art-091ce8d239304a0aaadc173be72f0bdf2022-12-21T20:16:23ZengElsevierRegenerative Therapy2352-32042018-06-0187379Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8Hitomi Fujisaki0Sugiko Futaki1Masashi Yamada2Kiyotoshi Sekiguchi3Toshihiko Hayashi4Takashi Ikejima5Shunji Hattori6Nippi Research Institute of Biomatrix, Toride, Ibaraki, Japan; Corresponding author. Nippi Research Institute of Biomatrix, 520-11 Kuwabara, Toride, Ibaraki, Japan.Department of Anatomy and Cell Biology, Osaka Medical College, Osaka, JapanDivision of Matrixome Research and Application, Institute for Protein Research, Osaka University, Suita, Osaka, JapanDivision of Matrixome Research and Application, Institute for Protein Research, Osaka University, Suita, Osaka, JapanChina-Japan Research Institute of Medical and Pharmaceutical Sciences, Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, ChinaChina-Japan Research Institute of Medical and Pharmaceutical Sciences, Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, ChinaNippi Research Institute of Biomatrix, Toride, Ibaraki, JapanKeratinocyte line cells HaCaT and FEPE1L-8 are used for skin model with type I collagen fibrils (gels). For this purpose, not only differentiation but also regulation of proliferation on type I collagen gels by exogenous calcium concentration is important. When exogenous calcium concentration is low, primary keratinocyte proliferation is repressed and eventually cells are induced to apoptosis on type I collagen gels. The apoptosis induced on type I collagen gels is suppressed by increasing calcium concentration in the medium. That is, higher exogenous calcium concentration is necessary for primary keratinocyte survival on type I collagen gels than for that on dish surface culture. Meanwhile much higher exogenous calcium causes cell differentiation and inhibition of proliferation. The optimal calcium concentrations for proliferation on type I collagen gels have not been clarified in keratinocyte line cells. HaCaT cells have a unique calcium sensitivity in comparison with primary keratinocytes, whereas FEPE1L-8 cells have a similar sensitivity to primary keratinocytes. In this study, we compared the effect of calcium concentrations on proliferation of HaCaT and FEPE1L-8 cells on type I collagen gels. On type I collagen gels, both line cells required higher calcium concentrations for proliferation than on dish surface. HaCaT cells proliferated better in a wider range of calcium concentrations than FEPE1L-8 cells. Keywords: Type I collagen gel, Keratinocyte proliferation, Calcium concentrationhttp://www.sciencedirect.com/science/article/pii/S2352320417300408 |
| spellingShingle | Hitomi Fujisaki Sugiko Futaki Masashi Yamada Kiyotoshi Sekiguchi Toshihiko Hayashi Takashi Ikejima Shunji Hattori Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8 Regenerative Therapy |
| title | Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8 |
| title_full | Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8 |
| title_fullStr | Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8 |
| title_full_unstemmed | Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8 |
| title_short | Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8 |
| title_sort | respective optimal calcium concentrations for proliferation on type i collagen fibrils in two keratinocyte line cells hacat and fepe1l 8 |
| url | http://www.sciencedirect.com/science/article/pii/S2352320417300408 |
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