GENETIC DIVERSITY INVESTIGATION AND MOLECULAR MARKERS ESTABLISHMENT FOR IDENTIFICATION OF SEVERAL INITIALLY SELECTIVE AVOCADO (Persea americana Miller) STRAINS IN LAMDONG PROVINCE
Morphologically and genetically investigation and estimation are necessary for train/cultivar identification and breeding in many crops. Materials from several selective avocado strains in Lamdong province were collected for genetic diversity analysis and strain identification. Initially morphologic...
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| Format: | Article |
| Language: | English |
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Dalat University
2017-01-01
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| Series: | Tạp chí Khoa học Đại học Đà Lạt |
| Subjects: | |
| Online Access: | http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/89 |
| _version_ | 1818282940025536512 |
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| author | Lê Ngọc Triệu Nguyễn Hoàng Phong Mai Tiến Đạt Thái Thạch Bích Nguyễn Thanh Tiền Lê Đình Vĩnh Bảo Nguyễn Khắc Quang Phan Ngọc Quỳnh Như |
| author_facet | Lê Ngọc Triệu Nguyễn Hoàng Phong Mai Tiến Đạt Thái Thạch Bích Nguyễn Thanh Tiền Lê Đình Vĩnh Bảo Nguyễn Khắc Quang Phan Ngọc Quỳnh Như |
| author_sort | Lê Ngọc Triệu |
| collection | DOAJ |
| description | Morphologically and genetically investigation and estimation are necessary for train/cultivar identification and breeding in many crops. Materials from several selective avocado strains in Lamdong province were collected for genetic diversity analysis and strain identification. Initially morphological characteristics of fruit and yield data of 11 avocado strains were recorded to contribute to data establishment for strain identification data. DNA fingerprints recorded with 10 ISSR primers including 125 electrophoresis DNA band were used for genetic diversity estimation of 11 investigated strains set, in the result, the average expected heterozygosity (gene diversity) was He = h = 0.3072, Shannon index was I = 0.4608, and percentage of polymorphic bands was PPB = 91.84%. Also with the same 10 ISSR primers, DNA fingerprints recorded from 18 samples (3 samples per strain) including 98 electrophoresis DNA band were used for identification 06 investigated avocado strains. Based on the occurrence or absence of specific electrophoresis DNA band, 9 single ISSR markers and 25 double/multiple ISSR markers were established to identify these 6 strains. These initially achieved results provide the necessary data for avocado breeding and development in general and identification 6 potential avocado strains. |
| first_indexed | 2024-12-13T00:28:59Z |
| format | Article |
| id | doaj.art-091e402c088a48929c255714b2645fed |
| institution | Directory Open Access Journal |
| issn | 0866-787X 0866-787X |
| language | English |
| last_indexed | 2024-12-13T00:28:59Z |
| publishDate | 2017-01-01 |
| publisher | Dalat University |
| record_format | Article |
| series | Tạp chí Khoa học Đại học Đà Lạt |
| spelling | doaj.art-091e402c088a48929c255714b2645fed2022-12-22T00:05:22ZengDalat UniversityTạp chí Khoa học Đại học Đà Lạt0866-787X0866-787X2017-01-016446748010.37569/DalatUniversity.6.4.89(2016)45GENETIC DIVERSITY INVESTIGATION AND MOLECULAR MARKERS ESTABLISHMENT FOR IDENTIFICATION OF SEVERAL INITIALLY SELECTIVE AVOCADO (Persea americana Miller) STRAINS IN LAMDONG PROVINCELê Ngọc Triệu0Nguyễn Hoàng Phong1Mai Tiến Đạt2Thái Thạch Bích3Nguyễn Thanh Tiền4Lê Đình Vĩnh Bảo5Nguyễn Khắc Quang6Phan Ngọc Quỳnh Như7Khoa Sinh học, Trường Đại học Đà LạtKhoa Sinh học, Trường Đại học Đà LạtKhoa Sinh học, Trường Đại học Đà LạtKhoa Sinh học, Trường Đại học Đà LạtKhoa Sinh học, Trường Đại học Đà LạtKhoa Sinh học, Trường Đại học Đà LạtKhoa Sinh học, Trường Đại học Đà LạtKhoa Sinh học, Trường Đại học Đà LạtMorphologically and genetically investigation and estimation are necessary for train/cultivar identification and breeding in many crops. Materials from several selective avocado strains in Lamdong province were collected for genetic diversity analysis and strain identification. Initially morphological characteristics of fruit and yield data of 11 avocado strains were recorded to contribute to data establishment for strain identification data. DNA fingerprints recorded with 10 ISSR primers including 125 electrophoresis DNA band were used for genetic diversity estimation of 11 investigated strains set, in the result, the average expected heterozygosity (gene diversity) was He = h = 0.3072, Shannon index was I = 0.4608, and percentage of polymorphic bands was PPB = 91.84%. Also with the same 10 ISSR primers, DNA fingerprints recorded from 18 samples (3 samples per strain) including 98 electrophoresis DNA band were used for identification 06 investigated avocado strains. Based on the occurrence or absence of specific electrophoresis DNA band, 9 single ISSR markers and 25 double/multiple ISSR markers were established to identify these 6 strains. These initially achieved results provide the necessary data for avocado breeding and development in general and identification 6 potential avocado strains.http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/89chỉ số shannonchỉ thị phân tửissrmức độ dị hợp trông đợi |
| spellingShingle | Lê Ngọc Triệu Nguyễn Hoàng Phong Mai Tiến Đạt Thái Thạch Bích Nguyễn Thanh Tiền Lê Đình Vĩnh Bảo Nguyễn Khắc Quang Phan Ngọc Quỳnh Như GENETIC DIVERSITY INVESTIGATION AND MOLECULAR MARKERS ESTABLISHMENT FOR IDENTIFICATION OF SEVERAL INITIALLY SELECTIVE AVOCADO (Persea americana Miller) STRAINS IN LAMDONG PROVINCE Tạp chí Khoa học Đại học Đà Lạt chỉ số shannon chỉ thị phân tử issr mức độ dị hợp trông đợi |
| title | GENETIC DIVERSITY INVESTIGATION AND MOLECULAR MARKERS ESTABLISHMENT FOR IDENTIFICATION OF SEVERAL INITIALLY SELECTIVE AVOCADO (Persea americana Miller) STRAINS IN LAMDONG PROVINCE |
| title_full | GENETIC DIVERSITY INVESTIGATION AND MOLECULAR MARKERS ESTABLISHMENT FOR IDENTIFICATION OF SEVERAL INITIALLY SELECTIVE AVOCADO (Persea americana Miller) STRAINS IN LAMDONG PROVINCE |
| title_fullStr | GENETIC DIVERSITY INVESTIGATION AND MOLECULAR MARKERS ESTABLISHMENT FOR IDENTIFICATION OF SEVERAL INITIALLY SELECTIVE AVOCADO (Persea americana Miller) STRAINS IN LAMDONG PROVINCE |
| title_full_unstemmed | GENETIC DIVERSITY INVESTIGATION AND MOLECULAR MARKERS ESTABLISHMENT FOR IDENTIFICATION OF SEVERAL INITIALLY SELECTIVE AVOCADO (Persea americana Miller) STRAINS IN LAMDONG PROVINCE |
| title_short | GENETIC DIVERSITY INVESTIGATION AND MOLECULAR MARKERS ESTABLISHMENT FOR IDENTIFICATION OF SEVERAL INITIALLY SELECTIVE AVOCADO (Persea americana Miller) STRAINS IN LAMDONG PROVINCE |
| title_sort | genetic diversity investigation and molecular markers establishment for identification of several initially selective avocado persea americana miller strains in lamdong province |
| topic | chỉ số shannon chỉ thị phân tử issr mức độ dị hợp trông đợi |
| url | http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/89 |
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