Nanoliter Centrifugal Liquid Dispenser Coupled with Superhydrophobic Microwell Array Chips for High-Throughput Cell Assays
Microfluidic systems have been regarded as a potential platform for high-throughput screening technology in drug discovery due to their low sample consumption, high integration, and easy operation. The handling of small-volume liquid is an essential operation in microfluidic systems, especially in i...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-06-01
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| Series: | Micromachines |
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| Online Access: | http://www.mdpi.com/2072-666X/9/6/286 |
| _version_ | 1819101081330778112 |
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| author | Yuyi Wang Yushuai Wu Yue Chen Jianxiong Zhang Xiaofang Chen Peng Liu |
| author_facet | Yuyi Wang Yushuai Wu Yue Chen Jianxiong Zhang Xiaofang Chen Peng Liu |
| author_sort | Yuyi Wang |
| collection | DOAJ |
| description | Microfluidic systems have been regarded as a potential platform for high-throughput screening technology in drug discovery due to their low sample consumption, high integration, and easy operation. The handling of small-volume liquid is an essential operation in microfluidic systems, especially in investigating large-scale combination conditions. Here, we develop a nanoliter centrifugal liquid dispenser (NanoCLD) coupled with superhydrophobic microwell array chips for high-throughput cell-based assays in the nanoliter scale. The NanoCLD consists of a plastic stock block with an array of drilled through holes, a reagent microwell array chip (reagent chip), and an alignment bottom assembled together in a fixture. A simple centrifugation at 800 rpm can dispense ~160 nL reagents into microwells in 5 min. The dispensed reagents are then delivered to cells by sandwiching the reagent chip upside down with another microwell array chip (cell chip) on which cells are cultured. A gradient of doxorubicin is then dispensed to the cell chip using the NanoCLD for validating the feasibility of performing drug tests on our microchip platform. This novel nanoliter-volume liquid dispensing method is simple, easy to operate, and especially suitable for repeatedly dispensing many different reagents simultaneously to microwells. |
| first_indexed | 2024-12-22T01:13:00Z |
| format | Article |
| id | doaj.art-09242ad0eccf425c9ff0a437f03e6d9d |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-22T01:13:00Z |
| publishDate | 2018-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-09242ad0eccf425c9ff0a437f03e6d9d2022-12-21T18:43:56ZengMDPI AGMicromachines2072-666X2018-06-019628610.3390/mi9060286mi9060286Nanoliter Centrifugal Liquid Dispenser Coupled with Superhydrophobic Microwell Array Chips for High-Throughput Cell AssaysYuyi Wang0Yushuai Wu1Yue Chen2Jianxiong Zhang3Xiaofang Chen4Peng Liu5Department of Biomedical Engineering, School of Medicine, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Tsinghua University, Beijing 100084, ChinaDepartment of Biomedical Engineering, School of Medicine, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Tsinghua University, Beijing 100084, ChinaDepartment of Biomedical Engineering, School of Medicine, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Tsinghua University, Beijing 100084, ChinaDepartment of Biomedical Engineering, School of Medicine, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Tsinghua University, Beijing 100084, ChinaSchool of Biological Science and Medical Engineering, Beihang University, Beijing 100191, ChinaDepartment of Biomedical Engineering, School of Medicine, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Tsinghua University, Beijing 100084, ChinaMicrofluidic systems have been regarded as a potential platform for high-throughput screening technology in drug discovery due to their low sample consumption, high integration, and easy operation. The handling of small-volume liquid is an essential operation in microfluidic systems, especially in investigating large-scale combination conditions. Here, we develop a nanoliter centrifugal liquid dispenser (NanoCLD) coupled with superhydrophobic microwell array chips for high-throughput cell-based assays in the nanoliter scale. The NanoCLD consists of a plastic stock block with an array of drilled through holes, a reagent microwell array chip (reagent chip), and an alignment bottom assembled together in a fixture. A simple centrifugation at 800 rpm can dispense ~160 nL reagents into microwells in 5 min. The dispensed reagents are then delivered to cells by sandwiching the reagent chip upside down with another microwell array chip (cell chip) on which cells are cultured. A gradient of doxorubicin is then dispensed to the cell chip using the NanoCLD for validating the feasibility of performing drug tests on our microchip platform. This novel nanoliter-volume liquid dispensing method is simple, easy to operate, and especially suitable for repeatedly dispensing many different reagents simultaneously to microwells.http://www.mdpi.com/2072-666X/9/6/286nanoliter liquid dispensingsuperhydrophobicitymicrowell arrayhigh-throughput screeningdrug screeningmicrofluidics |
| spellingShingle | Yuyi Wang Yushuai Wu Yue Chen Jianxiong Zhang Xiaofang Chen Peng Liu Nanoliter Centrifugal Liquid Dispenser Coupled with Superhydrophobic Microwell Array Chips for High-Throughput Cell Assays Micromachines nanoliter liquid dispensing superhydrophobicity microwell array high-throughput screening drug screening microfluidics |
| title | Nanoliter Centrifugal Liquid Dispenser Coupled with Superhydrophobic Microwell Array Chips for High-Throughput Cell Assays |
| title_full | Nanoliter Centrifugal Liquid Dispenser Coupled with Superhydrophobic Microwell Array Chips for High-Throughput Cell Assays |
| title_fullStr | Nanoliter Centrifugal Liquid Dispenser Coupled with Superhydrophobic Microwell Array Chips for High-Throughput Cell Assays |
| title_full_unstemmed | Nanoliter Centrifugal Liquid Dispenser Coupled with Superhydrophobic Microwell Array Chips for High-Throughput Cell Assays |
| title_short | Nanoliter Centrifugal Liquid Dispenser Coupled with Superhydrophobic Microwell Array Chips for High-Throughput Cell Assays |
| title_sort | nanoliter centrifugal liquid dispenser coupled with superhydrophobic microwell array chips for high throughput cell assays |
| topic | nanoliter liquid dispensing superhydrophobicity microwell array high-throughput screening drug screening microfluidics |
| url | http://www.mdpi.com/2072-666X/9/6/286 |
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