Comparison of the effects of exercise with chondroitin sulfate on knee osteoarthritis in rabbits
Abstract Background The aim of the study is to compare the effects of exercise therapy with chondroitin sulfate (CS) therapy in an experimental model of osteoarthritis (OA). Methods Twenty-one New Zealand rabbits were randomly divided into four groups: normal group (N group, n = 3); OA control group...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-01-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13018-018-0722-4 |
| _version_ | 1828151880190001152 |
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| author | Ning Ma Tingting Wang Lianyu Bie Yang Zhao Lidong Zhao Shai Zhang Li Gao Jianhua Xiao |
| author_facet | Ning Ma Tingting Wang Lianyu Bie Yang Zhao Lidong Zhao Shai Zhang Li Gao Jianhua Xiao |
| author_sort | Ning Ma |
| collection | DOAJ |
| description | Abstract Background The aim of the study is to compare the effects of exercise therapy with chondroitin sulfate (CS) therapy in an experimental model of osteoarthritis (OA). Methods Twenty-one New Zealand rabbits were randomly divided into four groups: normal group (N group, n = 3); OA control group (C group, n = 6); OA plus medication group (CS group, n = 6); and OA plus exercise group (E group, n = 6). Four weeks after modeling, the rabbits were subjected to exercise (artificial, 30 min/time, 4 times/week) or medicated with CS (2% CS, 0.3 ml/time, once/week) for 4 weeks. Histopathological changes in treated joints were examined after staining. X-ray and scanning electron microscopy was used to evaluate the different therapies by examining the surfaces and joint spaces of the articular cartilage. RT-qPCR was used to assess chondrogenic gene expression including Col2, Col10, mmp-13, il-1β, adamats-5, and acan in the experimental groups. Results Histology showed both treatment groups resulted in cartilage that was in good condition, with increased numbers of chondrocytes, and the results of X-ray and scanning electron microscopy showed the therapeutic effect of exercise therapy is equivalent to CS therapy, surface articular cartilage was flat, and the of cartilage layer was thinning. All treated groups induced the expression of Col10 and Col2 and decreased expression of mmp-13, il-1β, and adamats-5 compared with the control groups. The expression of acan was upregulated in the E group and downregulated in the CS group. Furthermore, expression of Col10 was higher and il-1β was lower in the exercise group compared to that of the CS group. Conclusion These results indicate that exercise has a positive effect on OA compare with CS, and it also supplies reference for the movement mode to improve function. |
| first_indexed | 2024-04-11T22:03:46Z |
| format | Article |
| id | doaj.art-092f876f3502449f892725bbaebbe61f |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T22:03:46Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-092f876f3502449f892725bbaebbe61f2022-12-22T04:00:48ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2018-01-011311910.1186/s13018-018-0722-4Comparison of the effects of exercise with chondroitin sulfate on knee osteoarthritis in rabbitsNing Ma0Tingting Wang1Lianyu Bie2Yang Zhao3Lidong Zhao4Shai Zhang5Li Gao6Jianhua Xiao7Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural UniversityKey Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural UniversityKey Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural UniversityKey Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural UniversityKey Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural UniversityKey Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural UniversityKey Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural UniversityKey Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural UniversityAbstract Background The aim of the study is to compare the effects of exercise therapy with chondroitin sulfate (CS) therapy in an experimental model of osteoarthritis (OA). Methods Twenty-one New Zealand rabbits were randomly divided into four groups: normal group (N group, n = 3); OA control group (C group, n = 6); OA plus medication group (CS group, n = 6); and OA plus exercise group (E group, n = 6). Four weeks after modeling, the rabbits were subjected to exercise (artificial, 30 min/time, 4 times/week) or medicated with CS (2% CS, 0.3 ml/time, once/week) for 4 weeks. Histopathological changes in treated joints were examined after staining. X-ray and scanning electron microscopy was used to evaluate the different therapies by examining the surfaces and joint spaces of the articular cartilage. RT-qPCR was used to assess chondrogenic gene expression including Col2, Col10, mmp-13, il-1β, adamats-5, and acan in the experimental groups. Results Histology showed both treatment groups resulted in cartilage that was in good condition, with increased numbers of chondrocytes, and the results of X-ray and scanning electron microscopy showed the therapeutic effect of exercise therapy is equivalent to CS therapy, surface articular cartilage was flat, and the of cartilage layer was thinning. All treated groups induced the expression of Col10 and Col2 and decreased expression of mmp-13, il-1β, and adamats-5 compared with the control groups. The expression of acan was upregulated in the E group and downregulated in the CS group. Furthermore, expression of Col10 was higher and il-1β was lower in the exercise group compared to that of the CS group. Conclusion These results indicate that exercise has a positive effect on OA compare with CS, and it also supplies reference for the movement mode to improve function.http://link.springer.com/article/10.1186/s13018-018-0722-4OsteoarthritisExerciseChondroitin sulfateRabbit |
| spellingShingle | Ning Ma Tingting Wang Lianyu Bie Yang Zhao Lidong Zhao Shai Zhang Li Gao Jianhua Xiao Comparison of the effects of exercise with chondroitin sulfate on knee osteoarthritis in rabbits Journal of Orthopaedic Surgery and Research Osteoarthritis Exercise Chondroitin sulfate Rabbit |
| title | Comparison of the effects of exercise with chondroitin sulfate on knee osteoarthritis in rabbits |
| title_full | Comparison of the effects of exercise with chondroitin sulfate on knee osteoarthritis in rabbits |
| title_fullStr | Comparison of the effects of exercise with chondroitin sulfate on knee osteoarthritis in rabbits |
| title_full_unstemmed | Comparison of the effects of exercise with chondroitin sulfate on knee osteoarthritis in rabbits |
| title_short | Comparison of the effects of exercise with chondroitin sulfate on knee osteoarthritis in rabbits |
| title_sort | comparison of the effects of exercise with chondroitin sulfate on knee osteoarthritis in rabbits |
| topic | Osteoarthritis Exercise Chondroitin sulfate Rabbit |
| url | http://link.springer.com/article/10.1186/s13018-018-0722-4 |
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