Evaluation and Immunogenicity of Combined Liposome-Based Vaccine Candidates against Hepatitis E and B Viruses in Rhesus Monkeys
The administration of vaccines using a combination approach ensures better coverage and reduces the number of injections and cost. The present study assessed liposome-complexed DNA-corresponding proteins of hepatitis E and B viruses (HEV and HBV) as combined vaccine candidates in rhesus monkeys. The...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/12/1/53 |
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| author | Tejaswini Deshmukh Rachita Shah Pradip Devhare Kavita Lole Vidya Arankalle |
| author_facet | Tejaswini Deshmukh Rachita Shah Pradip Devhare Kavita Lole Vidya Arankalle |
| author_sort | Tejaswini Deshmukh |
| collection | DOAJ |
| description | The administration of vaccines using a combination approach ensures better coverage and reduces the number of injections and cost. The present study assessed liposome-complexed DNA-corresponding proteins of hepatitis E and B viruses (HEV and HBV) as combined vaccine candidates in rhesus monkeys. The HEV and HBV components consisted of 450 bps, neutralizing the epitope/s (NE) region, and 685 bps small (S) envelope gene-corresponding proteins, respectively. Three groups (<i>n</i> = 2 monkeys/group) were intramuscularly immunized with a total of three doses of NE Protein (Lipo-NE-P), NE DNA + Protein (Lipo-NE-DP), and each of NE and S DNA + Protein (Lipo-NES-DP), respectively, given one month apart. All immunized monkeys were challenged with 10,000 fifty percent monkey infectious dose of homologous HEV strain. Post-immunization anti-HEV antibody levels in monkeys were 59.4 and 148.4 IU/mL (Lipo-NE-P), 177.0 and 240.8 IU/mL (Lipo-NE-DP), and 240.7 and 164.9 IU/mL (Lipo-NES-DP). Anti-HBV antibody levels in Lipo-NES-DP immunized monkeys were 58,786 and 6213 mIU/mL. None of the challenged monkeys showed viremia and elevation in serum alanine amino transferase levels. Monkeys immunized with Lipo-NE-DP and Lipo-NES-DP exhibited a sterilizing immunity, indicating complete protection, whereas monkeys immunized with Lipo-NE-P showed limited viral replication. In conclusion, the liposome-complexed DNA-corresponding proteins of HEV and HBV induced protective humoral immune responses to both components in monkeys and are worth exploring further. |
| first_indexed | 2024-03-08T09:44:18Z |
| format | Article |
| id | doaj.art-09355e2ac18146fc9f1076fd7684ce7d |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-08T09:44:18Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-09355e2ac18146fc9f1076fd7684ce7d2024-01-29T14:25:36ZengMDPI AGVaccines2076-393X2024-01-011215310.3390/vaccines12010053Evaluation and Immunogenicity of Combined Liposome-Based Vaccine Candidates against Hepatitis E and B Viruses in Rhesus MonkeysTejaswini Deshmukh0Rachita Shah1Pradip Devhare2Kavita Lole3Vidya Arankalle4Hepatitis Group, ICMR-National Institute of Virology, 130/1, Pune 411021, IndiaHepatitis Group, ICMR-National Institute of Virology, 130/1, Pune 411021, IndiaHepatitis Group, ICMR-National Institute of Virology, 130/1, Pune 411021, IndiaHepatitis Group, ICMR-National Institute of Virology, 130/1, Pune 411021, IndiaHepatitis Group, ICMR-National Institute of Virology, 130/1, Pune 411021, IndiaThe administration of vaccines using a combination approach ensures better coverage and reduces the number of injections and cost. The present study assessed liposome-complexed DNA-corresponding proteins of hepatitis E and B viruses (HEV and HBV) as combined vaccine candidates in rhesus monkeys. The HEV and HBV components consisted of 450 bps, neutralizing the epitope/s (NE) region, and 685 bps small (S) envelope gene-corresponding proteins, respectively. Three groups (<i>n</i> = 2 monkeys/group) were intramuscularly immunized with a total of three doses of NE Protein (Lipo-NE-P), NE DNA + Protein (Lipo-NE-DP), and each of NE and S DNA + Protein (Lipo-NES-DP), respectively, given one month apart. All immunized monkeys were challenged with 10,000 fifty percent monkey infectious dose of homologous HEV strain. Post-immunization anti-HEV antibody levels in monkeys were 59.4 and 148.4 IU/mL (Lipo-NE-P), 177.0 and 240.8 IU/mL (Lipo-NE-DP), and 240.7 and 164.9 IU/mL (Lipo-NES-DP). Anti-HBV antibody levels in Lipo-NES-DP immunized monkeys were 58,786 and 6213 mIU/mL. None of the challenged monkeys showed viremia and elevation in serum alanine amino transferase levels. Monkeys immunized with Lipo-NE-DP and Lipo-NES-DP exhibited a sterilizing immunity, indicating complete protection, whereas monkeys immunized with Lipo-NE-P showed limited viral replication. In conclusion, the liposome-complexed DNA-corresponding proteins of HEV and HBV induced protective humoral immune responses to both components in monkeys and are worth exploring further.https://www.mdpi.com/2076-393X/12/1/53hepatitis Ehepatitis Bneutralizing epitope/ssmall envelopevaccine candidatesliposomes |
| spellingShingle | Tejaswini Deshmukh Rachita Shah Pradip Devhare Kavita Lole Vidya Arankalle Evaluation and Immunogenicity of Combined Liposome-Based Vaccine Candidates against Hepatitis E and B Viruses in Rhesus Monkeys Vaccines hepatitis E hepatitis B neutralizing epitope/s small envelope vaccine candidates liposomes |
| title | Evaluation and Immunogenicity of Combined Liposome-Based Vaccine Candidates against Hepatitis E and B Viruses in Rhesus Monkeys |
| title_full | Evaluation and Immunogenicity of Combined Liposome-Based Vaccine Candidates against Hepatitis E and B Viruses in Rhesus Monkeys |
| title_fullStr | Evaluation and Immunogenicity of Combined Liposome-Based Vaccine Candidates against Hepatitis E and B Viruses in Rhesus Monkeys |
| title_full_unstemmed | Evaluation and Immunogenicity of Combined Liposome-Based Vaccine Candidates against Hepatitis E and B Viruses in Rhesus Monkeys |
| title_short | Evaluation and Immunogenicity of Combined Liposome-Based Vaccine Candidates against Hepatitis E and B Viruses in Rhesus Monkeys |
| title_sort | evaluation and immunogenicity of combined liposome based vaccine candidates against hepatitis e and b viruses in rhesus monkeys |
| topic | hepatitis E hepatitis B neutralizing epitope/s small envelope vaccine candidates liposomes |
| url | https://www.mdpi.com/2076-393X/12/1/53 |
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