Zona pellucida treatment before CRISPR/Cas9‐mediated genome editing of porcine zygotes

Zona pellucida treatment before CRISPR/Cas9‐mediated genome editing of porcine zygotes

Abstract Background Increasing the permeability of the zona pellucida (ZP) of oocytes before CRISPR/Cas9 electroporation may improve the efficiency of gene editing; however, the effects of this approach on subsequent developmental processes are unclear. In this study, the effects of ZP treatment bef...

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Bibliographic Details
Main Authors: Zhao Namula, Maki Hirata, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Naoaki Yoshimura, Fuminori Tanihara, Chommanart Thongkittidilok, Takeshige Otoi
Format: Article
Language:English
Published: Wiley 2022-01-01
Series:Veterinary Medicine and Science
Subjects:
CRISPR
electroporation
genome editing
porcine zygote
zona pellucida
Online Access:https://doi.org/10.1002/vms3.659

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