Exploration and identification of six novel ferroptosis-related hub genes as potential gene signatures for peripheral nerve injury
Specific biomarkers of ferroptosis after peripheral nerve injury (PNI) are still under debate. In this study, 52 differentially expressed ferroptosis-related genes (DE-FRGs) were retrieved from publicly accessible sequencing data of intact and injured samples of rats with sciatic nerve crush injury....
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| Format: | Article |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2023.1156467/full |
| _version_ | 1797851346654724096 |
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| author | Yifei Zhang Yifei Zhang Yifei Zhang Chun Chen Chun Chen Chun Chen Dawei Li Dawei Li Dawei Li Penghui Chen Penghui Chen Penghui Chen Lei Hang Jun Yang Jun Yang Jun Yang Jin Xie Jin Xie Jin Xie |
| author_facet | Yifei Zhang Yifei Zhang Yifei Zhang Chun Chen Chun Chen Chun Chen Dawei Li Dawei Li Dawei Li Penghui Chen Penghui Chen Penghui Chen Lei Hang Jun Yang Jun Yang Jun Yang Jin Xie Jin Xie Jin Xie |
| author_sort | Yifei Zhang |
| collection | DOAJ |
| description | Specific biomarkers of ferroptosis after peripheral nerve injury (PNI) are still under debate. In this study, 52 differentially expressed ferroptosis-related genes (DE-FRGs) were retrieved from publicly accessible sequencing data of intact and injured samples of rats with sciatic nerve crush injury. Functional enrichment analyses revealed that adipogenesis, mitochondrial gene sets, and pathways of MAPK, p53, and CD28 family were predominantly engaged in ferroptosis after PNI. Next, Cdkn1a, Cdh1, Hif1a, Hmox1, Nfe2l2, and Tgfb1 were investigated as new ferroptosis-associated hub genes after PNI. Subsequently, clustering correlation heatmap shows six hub genes are linked to mitochondria. The immunofluorescence assay at 0, 1, 4, 7, and 14 days indicated the temporal expression patterns of Tgfb1, Hmox1, and Hif1a after PNI were consistent with ferroptosis validated by PI and ROS staining, while Cdh1, Cdkn1a, and Nfe2l2 were the opposite. In summary, this study identified six hub genes as possible ferroptosis-related biomarkers for PNI, which may offer therapeutic targets for peripheral nerve regeneration and provide a therapeutic window for ferroptosis. |
| first_indexed | 2024-04-09T19:16:26Z |
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| id | doaj.art-09467ceb92864110a0d5a5fa2622f9bb |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-09T19:16:26Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-09467ceb92864110a0d5a5fa2622f9bb2023-04-06T05:02:52ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-04-011410.3389/fgene.2023.11564671156467Exploration and identification of six novel ferroptosis-related hub genes as potential gene signatures for peripheral nerve injuryYifei Zhang0Yifei Zhang1Yifei Zhang2Chun Chen3Chun Chen4Chun Chen5Dawei Li6Dawei Li7Dawei Li8Penghui Chen9Penghui Chen10Penghui Chen11Lei Hang12Jun Yang13Jun Yang14Jun Yang15Jin Xie16Jin Xie17Jin Xie18Department of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaEar Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, ChinaDepartment of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaEar Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, ChinaDepartment of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaEar Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, ChinaDepartment of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaEar Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, ChinaBusiness School, Tianhua College, Shanghai Normal University, Shanghai, ChinaDepartment of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaEar Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, ChinaDepartment of Otorhinolaryngology-Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaEar Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, ChinaSpecific biomarkers of ferroptosis after peripheral nerve injury (PNI) are still under debate. In this study, 52 differentially expressed ferroptosis-related genes (DE-FRGs) were retrieved from publicly accessible sequencing data of intact and injured samples of rats with sciatic nerve crush injury. Functional enrichment analyses revealed that adipogenesis, mitochondrial gene sets, and pathways of MAPK, p53, and CD28 family were predominantly engaged in ferroptosis after PNI. Next, Cdkn1a, Cdh1, Hif1a, Hmox1, Nfe2l2, and Tgfb1 were investigated as new ferroptosis-associated hub genes after PNI. Subsequently, clustering correlation heatmap shows six hub genes are linked to mitochondria. The immunofluorescence assay at 0, 1, 4, 7, and 14 days indicated the temporal expression patterns of Tgfb1, Hmox1, and Hif1a after PNI were consistent with ferroptosis validated by PI and ROS staining, while Cdh1, Cdkn1a, and Nfe2l2 were the opposite. In summary, this study identified six hub genes as possible ferroptosis-related biomarkers for PNI, which may offer therapeutic targets for peripheral nerve regeneration and provide a therapeutic window for ferroptosis.https://www.frontiersin.org/articles/10.3389/fgene.2023.1156467/fullperipheral nerve regenerationferroptosisbiomarkerbioinformaticshub geneperipheral nerve injury |
| spellingShingle | Yifei Zhang Yifei Zhang Yifei Zhang Chun Chen Chun Chen Chun Chen Dawei Li Dawei Li Dawei Li Penghui Chen Penghui Chen Penghui Chen Lei Hang Jun Yang Jun Yang Jun Yang Jin Xie Jin Xie Jin Xie Exploration and identification of six novel ferroptosis-related hub genes as potential gene signatures for peripheral nerve injury Frontiers in Genetics peripheral nerve regeneration ferroptosis biomarker bioinformatics hub gene peripheral nerve injury |
| title | Exploration and identification of six novel ferroptosis-related hub genes as potential gene signatures for peripheral nerve injury |
| title_full | Exploration and identification of six novel ferroptosis-related hub genes as potential gene signatures for peripheral nerve injury |
| title_fullStr | Exploration and identification of six novel ferroptosis-related hub genes as potential gene signatures for peripheral nerve injury |
| title_full_unstemmed | Exploration and identification of six novel ferroptosis-related hub genes as potential gene signatures for peripheral nerve injury |
| title_short | Exploration and identification of six novel ferroptosis-related hub genes as potential gene signatures for peripheral nerve injury |
| title_sort | exploration and identification of six novel ferroptosis related hub genes as potential gene signatures for peripheral nerve injury |
| topic | peripheral nerve regeneration ferroptosis biomarker bioinformatics hub gene peripheral nerve injury |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2023.1156467/full |
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