An Ovine Model for Percutaneous Pulmonary Artery Laser Denervation: Perivascular Innervation and Ablation Lesion Characteristics
Background: Pulmonary artery denervation (PADN) is an evolving interventional procedure capable to reduce pulmonary artery (PA) pressure. We aimed to compare PA nerve distribution in different specimens and assess the feasibility of an ovine model for a denervation procedure and evaluate the acute c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/16/8788 |
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| author | Heber Ivan Condori Leandro Elena G. Koshevaya Lubov B. Mitrofanova Aleksandr D. Vakhrushev Natalia S. Goncharova Lev E. Korobchenko Elizaveta M. Andreeva Dmitry S. Lebedev Evgeny N. Mikhaylov |
| author_facet | Heber Ivan Condori Leandro Elena G. Koshevaya Lubov B. Mitrofanova Aleksandr D. Vakhrushev Natalia S. Goncharova Lev E. Korobchenko Elizaveta M. Andreeva Dmitry S. Lebedev Evgeny N. Mikhaylov |
| author_sort | Heber Ivan Condori Leandro |
| collection | DOAJ |
| description | Background: Pulmonary artery denervation (PADN) is an evolving interventional procedure capable to reduce pulmonary artery (PA) pressure. We aimed to compare PA nerve distribution in different specimens and assess the feasibility of an ovine model for a denervation procedure and evaluate the acute changes induced by laser energy. Methods: The experiment was divided into two phases: (1) the analysis of PA nerve distribution in sheep, pigs, and humans using histological and immunochemical methods; (2) fiberoptic PADN in sheep and postmortem laser lesion characteristics. Results: PA nerve density and distribution in sheep differ from humans, although pigs and sheep share similar characteristics, nerve fibers are observed in the media layer, adventitia, and perivascular tissue in sheep. Necrosis of the intima and focal hemorrhages within the media, adventitia, and perivascular adipose tissue were evidenced post laser PADN. Among the identified lesions, 40% reached adventitia and could be classified as effective for PADN. The use of 20 W ablation energy was safer and 30 W-ablation led to collateral organ damage. Conclusions: An ovine model is suitable for PADN procedures; however, nerve distribution in the PA bifurcation and main branches differ from human PA innervation. Laser ablation can be safely used for PADN procedures. |
| first_indexed | 2024-03-10T08:44:29Z |
| format | Article |
| id | doaj.art-094b26d63460453b9fef495021fb6672 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:44:29Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-094b26d63460453b9fef495021fb66722023-11-22T08:00:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216878810.3390/ijms22168788An Ovine Model for Percutaneous Pulmonary Artery Laser Denervation: Perivascular Innervation and Ablation Lesion CharacteristicsHeber Ivan Condori Leandro0Elena G. Koshevaya1Lubov B. Mitrofanova2Aleksandr D. Vakhrushev3Natalia S. Goncharova4Lev E. Korobchenko5Elizaveta M. Andreeva6Dmitry S. Lebedev7Evgeny N. Mikhaylov8Almazov National Medical Research Centre, 2 Akkuratova Str., 197341 Saint-Petersburg, RussiaAlmazov National Medical Research Centre, 2 Akkuratova Str., 197341 Saint-Petersburg, RussiaAlmazov National Medical Research Centre, 2 Akkuratova Str., 197341 Saint-Petersburg, RussiaAlmazov National Medical Research Centre, 2 Akkuratova Str., 197341 Saint-Petersburg, RussiaAlmazov National Medical Research Centre, 2 Akkuratova Str., 197341 Saint-Petersburg, RussiaAlmazov National Medical Research Centre, 2 Akkuratova Str., 197341 Saint-Petersburg, RussiaAlmazov National Medical Research Centre, 2 Akkuratova Str., 197341 Saint-Petersburg, RussiaAlmazov National Medical Research Centre, 2 Akkuratova Str., 197341 Saint-Petersburg, RussiaAlmazov National Medical Research Centre, 2 Akkuratova Str., 197341 Saint-Petersburg, RussiaBackground: Pulmonary artery denervation (PADN) is an evolving interventional procedure capable to reduce pulmonary artery (PA) pressure. We aimed to compare PA nerve distribution in different specimens and assess the feasibility of an ovine model for a denervation procedure and evaluate the acute changes induced by laser energy. Methods: The experiment was divided into two phases: (1) the analysis of PA nerve distribution in sheep, pigs, and humans using histological and immunochemical methods; (2) fiberoptic PADN in sheep and postmortem laser lesion characteristics. Results: PA nerve density and distribution in sheep differ from humans, although pigs and sheep share similar characteristics, nerve fibers are observed in the media layer, adventitia, and perivascular tissue in sheep. Necrosis of the intima and focal hemorrhages within the media, adventitia, and perivascular adipose tissue were evidenced post laser PADN. Among the identified lesions, 40% reached adventitia and could be classified as effective for PADN. The use of 20 W ablation energy was safer and 30 W-ablation led to collateral organ damage. Conclusions: An ovine model is suitable for PADN procedures; however, nerve distribution in the PA bifurcation and main branches differ from human PA innervation. Laser ablation can be safely used for PADN procedures.https://www.mdpi.com/1422-0067/22/16/8788pulmonary hypertensionpulmonary artery denervationlaser ablationS100 immunolabelinganimal model |
| spellingShingle | Heber Ivan Condori Leandro Elena G. Koshevaya Lubov B. Mitrofanova Aleksandr D. Vakhrushev Natalia S. Goncharova Lev E. Korobchenko Elizaveta M. Andreeva Dmitry S. Lebedev Evgeny N. Mikhaylov An Ovine Model for Percutaneous Pulmonary Artery Laser Denervation: Perivascular Innervation and Ablation Lesion Characteristics International Journal of Molecular Sciences pulmonary hypertension pulmonary artery denervation laser ablation S100 immunolabeling animal model |
| title | An Ovine Model for Percutaneous Pulmonary Artery Laser Denervation: Perivascular Innervation and Ablation Lesion Characteristics |
| title_full | An Ovine Model for Percutaneous Pulmonary Artery Laser Denervation: Perivascular Innervation and Ablation Lesion Characteristics |
| title_fullStr | An Ovine Model for Percutaneous Pulmonary Artery Laser Denervation: Perivascular Innervation and Ablation Lesion Characteristics |
| title_full_unstemmed | An Ovine Model for Percutaneous Pulmonary Artery Laser Denervation: Perivascular Innervation and Ablation Lesion Characteristics |
| title_short | An Ovine Model for Percutaneous Pulmonary Artery Laser Denervation: Perivascular Innervation and Ablation Lesion Characteristics |
| title_sort | ovine model for percutaneous pulmonary artery laser denervation perivascular innervation and ablation lesion characteristics |
| topic | pulmonary hypertension pulmonary artery denervation laser ablation S100 immunolabeling animal model |
| url | https://www.mdpi.com/1422-0067/22/16/8788 |
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