Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning
This study aimed to evaluate the agronomic performance of cassava genotypes from the in vitro shoot tip culture to eliminate the cassava frogskin disease for several root and aerial part characteristics. Cassava plants from accessions BGM0315, BGM0464 and BGM0841 infected with cassava frogskin dise...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Uberlândia
2017-09-01
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| Series: | Bioscience Journal |
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| Online Access: | https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/36585 |
| _version_ | 1818445682282856448 |
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| author | Mariane de Jesus da Silva de Carvalho Eder Jorge de Oliveira Antônio da Silva Souza Luziane Brandão Alves Marcela Tonini Venturini |
| author_facet | Mariane de Jesus da Silva de Carvalho Eder Jorge de Oliveira Antônio da Silva Souza Luziane Brandão Alves Marcela Tonini Venturini |
| author_sort | Mariane de Jesus da Silva de Carvalho |
| collection | DOAJ |
| description |
This study aimed to evaluate the agronomic performance of cassava genotypes from the in vitro shoot tip culture to eliminate the cassava frogskin disease for several root and aerial part characteristics. Cassava plants from accessions BGM0315, BGM0464 and BGM0841 infected with cassava frogskin disease were grown in a greenhouse after clonal cleaning. Cuttings from the three accessions were subjected to tetracycline concentrations (0, 5, 10 and 15 mg L-1) for three minutes, and then maintained in an acclimatized chamber (35 ± 1 °C and 16 hour photoperiod). Shoots were disinfected for excising shoot tips (0.2 mm and 0.4 mm) and inoculated in a culture medium containing the same concentrations of tetracycline used in the cuttings. After 60 days of cultivation, the explants were transferred to medium without antibiotic, 30 days later they were acclimatized for a period of 70 days for subsequent planting in the field. Seven months after planting, agronomical evaluation was held for root and aerial part characteristics. No influence of isolated shoot tip size was noticed on agronomic characteristics, while the addition of tetracycline in the culture medium, specifically at the concentrations of 5 mg L-1 and 15 mg L-1, was favorable to the development of the root system of plants in the field. The results revealed that the agronomic performance of cassava plants derived from in vitro cultivation are higher for the production of basic propagation material for the following production cycles, as well as root production for commercial use with subsequent generation of income.
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| first_indexed | 2024-12-14T19:35:42Z |
| format | Article |
| id | doaj.art-094dfa09b1504f5db2390567d215f700 |
| institution | Directory Open Access Journal |
| issn | 1981-3163 |
| language | English |
| last_indexed | 2024-12-14T19:35:42Z |
| publishDate | 2017-09-01 |
| publisher | Universidade Federal de Uberlândia |
| record_format | Article |
| series | Bioscience Journal |
| spelling | doaj.art-094dfa09b1504f5db2390567d215f7002022-12-21T22:49:54ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632017-09-01335Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning Mariane de Jesus da Silva de Carvalho0Eder Jorge de OliveiraAntônio da Silva Souza1Luziane Brandão Alves2Marcela Tonini Venturini3Centro de Ciências Agrárias Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia, Cruz das Almas, BA, BrazilPesquisador da Embrapa Mandioca e Fruticultura, Rua Embrapa, s/nº CP007, Cruz das Almas, BA - 44380-000Centro de Ciências Agrárias Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia, Cruz das Almas, BA, BrazilCentro de Ciências Agrárias Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia, Cruz das Almas, BA, Brazil This study aimed to evaluate the agronomic performance of cassava genotypes from the in vitro shoot tip culture to eliminate the cassava frogskin disease for several root and aerial part characteristics. Cassava plants from accessions BGM0315, BGM0464 and BGM0841 infected with cassava frogskin disease were grown in a greenhouse after clonal cleaning. Cuttings from the three accessions were subjected to tetracycline concentrations (0, 5, 10 and 15 mg L-1) for three minutes, and then maintained in an acclimatized chamber (35 ± 1 °C and 16 hour photoperiod). Shoots were disinfected for excising shoot tips (0.2 mm and 0.4 mm) and inoculated in a culture medium containing the same concentrations of tetracycline used in the cuttings. After 60 days of cultivation, the explants were transferred to medium without antibiotic, 30 days later they were acclimatized for a period of 70 days for subsequent planting in the field. Seven months after planting, agronomical evaluation was held for root and aerial part characteristics. No influence of isolated shoot tip size was noticed on agronomic characteristics, while the addition of tetracycline in the culture medium, specifically at the concentrations of 5 mg L-1 and 15 mg L-1, was favorable to the development of the root system of plants in the field. The results revealed that the agronomic performance of cassava plants derived from in vitro cultivation are higher for the production of basic propagation material for the following production cycles, as well as root production for commercial use with subsequent generation of income. https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/36585Manihot esculent CrantzIn vitro cultivationClonal cleaningStarch production |
| spellingShingle | Mariane de Jesus da Silva de Carvalho Eder Jorge de Oliveira Antônio da Silva Souza Luziane Brandão Alves Marcela Tonini Venturini Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning Bioscience Journal Manihot esculent Crantz In vitro cultivation Clonal cleaning Starch production |
| title | Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning |
| title_full | Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning |
| title_fullStr | Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning |
| title_full_unstemmed | Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning |
| title_short | Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning |
| title_sort | agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning |
| topic | Manihot esculent Crantz In vitro cultivation Clonal cleaning Starch production |
| url | https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/36585 |
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