Genetic diversity revealed by AFLP markers in Albanian goat breeds
The amplified fragment length polymorphism (AFLP) technique with three EcoRI/TaqI primer combinations was used in 185 unrelated individuals, representative of 6 local goat breeds of Albania, and 107 markers were generated. The mean Nei’s expected heterozygosity value for the whole population was...
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Format: | Article |
Language: | English |
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University of Belgrade, University of Novi Sad
2012-01-01
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Series: | Archives of Biological Sciences |
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Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641202799H.pdf |
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author | Hoda Anila Sena Lumturi Hykaj Gentian |
author_facet | Hoda Anila Sena Lumturi Hykaj Gentian |
author_sort | Hoda Anila |
collection | DOAJ |
description | The amplified fragment length polymorphism (AFLP) technique with three EcoRI/TaqI primer combinations was used in 185 unrelated individuals, representative of 6 local goat breeds of Albania, and 107 markers were generated. The mean Nei’s expected heterozygosity value for the whole population was 0.199 and the mean Shannon index was 0.249, indicating a high level of within-breed diversity. Wright’s FST index, Nei’s unbiased genetic distance and Reynolds’ genetic distance were calculated. Pairwise Fst values among the populations ranged from 0.019 to 0.047. A highly significant average FST of 0.031 was estimated, showing a low level of breed subdivision. Most of the variation is accounted for by differences among individuals. Cluster analysis based on Reynolds’ genetic distance between breeds and PCA were performed. An individual UPGMA tree based on Jaccard’s similarity index showed clusters with individuals from all goat breeds. Analysis of population structure points to a high level of admixture among breeds. |
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format | Article |
id | doaj.art-094fb36727e049edbf2d865079ddc9da |
institution | Directory Open Access Journal |
issn | 0354-4664 1821-4339 |
language | English |
last_indexed | 2024-12-20T03:41:56Z |
publishDate | 2012-01-01 |
publisher | University of Belgrade, University of Novi Sad |
record_format | Article |
series | Archives of Biological Sciences |
spelling | doaj.art-094fb36727e049edbf2d865079ddc9da2022-12-21T19:54:43ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392012-01-0164279980710.2298/ABS1202799HGenetic diversity revealed by AFLP markers in Albanian goat breedsHoda AnilaSena LumturiHykaj GentianThe amplified fragment length polymorphism (AFLP) technique with three EcoRI/TaqI primer combinations was used in 185 unrelated individuals, representative of 6 local goat breeds of Albania, and 107 markers were generated. The mean Nei’s expected heterozygosity value for the whole population was 0.199 and the mean Shannon index was 0.249, indicating a high level of within-breed diversity. Wright’s FST index, Nei’s unbiased genetic distance and Reynolds’ genetic distance were calculated. Pairwise Fst values among the populations ranged from 0.019 to 0.047. A highly significant average FST of 0.031 was estimated, showing a low level of breed subdivision. Most of the variation is accounted for by differences among individuals. Cluster analysis based on Reynolds’ genetic distance between breeds and PCA were performed. An individual UPGMA tree based on Jaccard’s similarity index showed clusters with individuals from all goat breeds. Analysis of population structure points to a high level of admixture among breeds.http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641202799H.pdflocal breedgoatgenetic variabilitygenetic diversitypopulation structureAlbania |
spellingShingle | Hoda Anila Sena Lumturi Hykaj Gentian Genetic diversity revealed by AFLP markers in Albanian goat breeds Archives of Biological Sciences local breed goat genetic variability genetic diversity population structure Albania |
title | Genetic diversity revealed by AFLP markers in Albanian goat breeds |
title_full | Genetic diversity revealed by AFLP markers in Albanian goat breeds |
title_fullStr | Genetic diversity revealed by AFLP markers in Albanian goat breeds |
title_full_unstemmed | Genetic diversity revealed by AFLP markers in Albanian goat breeds |
title_short | Genetic diversity revealed by AFLP markers in Albanian goat breeds |
title_sort | genetic diversity revealed by aflp markers in albanian goat breeds |
topic | local breed goat genetic variability genetic diversity population structure Albania |
url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641202799H.pdf |
work_keys_str_mv | AT hodaanila geneticdiversityrevealedbyaflpmarkersinalbaniangoatbreeds AT senalumturi geneticdiversityrevealedbyaflpmarkersinalbaniangoatbreeds AT hykajgentian geneticdiversityrevealedbyaflpmarkersinalbaniangoatbreeds |