Genetic diversity revealed by AFLP markers in Albanian goat breeds

The amplified fragment length polymorphism (AFLP) technique with three EcoRI/TaqI primer combinations was used in 185 unrelated individuals, representative of 6 local goat breeds of Albania, and 107 markers were generated. The mean Nei’s expected heterozygosity value for the whole population was...

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Main Authors: Hoda Anila, Sena Lumturi, Hykaj Gentian
Format: Article
Language:English
Published: University of Belgrade, University of Novi Sad 2012-01-01
Series:Archives of Biological Sciences
Subjects:
Online Access:http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641202799H.pdf
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author Hoda Anila
Sena Lumturi
Hykaj Gentian
author_facet Hoda Anila
Sena Lumturi
Hykaj Gentian
author_sort Hoda Anila
collection DOAJ
description The amplified fragment length polymorphism (AFLP) technique with three EcoRI/TaqI primer combinations was used in 185 unrelated individuals, representative of 6 local goat breeds of Albania, and 107 markers were generated. The mean Nei’s expected heterozygosity value for the whole population was 0.199 and the mean Shannon index was 0.249, indicating a high level of within-breed diversity. Wright’s FST index, Nei’s unbiased genetic distance and Reynolds’ genetic distance were calculated. Pairwise Fst values among the populations ranged from 0.019 to 0.047. A highly significant average FST of 0.031 was estimated, showing a low level of breed subdivision. Most of the variation is accounted for by differences among individuals. Cluster analysis based on Reynolds’ genetic distance between breeds and PCA were performed. An individual UPGMA tree based on Jaccard’s similarity index showed clusters with individuals from all goat breeds. Analysis of population structure points to a high level of admixture among breeds.
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spelling doaj.art-094fb36727e049edbf2d865079ddc9da2022-12-21T19:54:43ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392012-01-0164279980710.2298/ABS1202799HGenetic diversity revealed by AFLP markers in Albanian goat breedsHoda AnilaSena LumturiHykaj GentianThe amplified fragment length polymorphism (AFLP) technique with three EcoRI/TaqI primer combinations was used in 185 unrelated individuals, representative of 6 local goat breeds of Albania, and 107 markers were generated. The mean Nei’s expected heterozygosity value for the whole population was 0.199 and the mean Shannon index was 0.249, indicating a high level of within-breed diversity. Wright’s FST index, Nei’s unbiased genetic distance and Reynolds’ genetic distance were calculated. Pairwise Fst values among the populations ranged from 0.019 to 0.047. A highly significant average FST of 0.031 was estimated, showing a low level of breed subdivision. Most of the variation is accounted for by differences among individuals. Cluster analysis based on Reynolds’ genetic distance between breeds and PCA were performed. An individual UPGMA tree based on Jaccard’s similarity index showed clusters with individuals from all goat breeds. Analysis of population structure points to a high level of admixture among breeds.http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641202799H.pdflocal breedgoatgenetic variabilitygenetic diversitypopulation structureAlbania
spellingShingle Hoda Anila
Sena Lumturi
Hykaj Gentian
Genetic diversity revealed by AFLP markers in Albanian goat breeds
Archives of Biological Sciences
local breed
goat
genetic variability
genetic diversity
population structure
Albania
title Genetic diversity revealed by AFLP markers in Albanian goat breeds
title_full Genetic diversity revealed by AFLP markers in Albanian goat breeds
title_fullStr Genetic diversity revealed by AFLP markers in Albanian goat breeds
title_full_unstemmed Genetic diversity revealed by AFLP markers in Albanian goat breeds
title_short Genetic diversity revealed by AFLP markers in Albanian goat breeds
title_sort genetic diversity revealed by aflp markers in albanian goat breeds
topic local breed
goat
genetic variability
genetic diversity
population structure
Albania
url http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641202799H.pdf
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