Bacterial biofilm under flow: First a physical struggle to stay, then a matter of breathing.
Bacterial communities attached to surfaces under fluid flow represent a widespread lifestyle of the microbial world. Through shear stress generation and molecular transport regulation, hydrodynamics conveys effects that are very different by nature but strongly coupled. To decipher the influence of...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5389662?pdf=render |
| _version_ | 1828924985035456512 |
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| author | Philippe Thomen Jérôme Robert Amaury Monmeyran Anne-Florence Bitbol Carine Douarche Nelly Henry |
| author_facet | Philippe Thomen Jérôme Robert Amaury Monmeyran Anne-Florence Bitbol Carine Douarche Nelly Henry |
| author_sort | Philippe Thomen |
| collection | DOAJ |
| description | Bacterial communities attached to surfaces under fluid flow represent a widespread lifestyle of the microbial world. Through shear stress generation and molecular transport regulation, hydrodynamics conveys effects that are very different by nature but strongly coupled. To decipher the influence of these levers on bacterial biofilms immersed in moving fluids, we quantitatively and simultaneously investigated physicochemical and biological properties of the biofilm. We designed a millifluidic setup allowing to control hydrodynamic conditions and to monitor biofilm development in real time using microscope imaging. We also conducted a transcriptomic analysis to detect a potential physiological response to hydrodynamics. We discovered that a threshold value of shear stress determined biofilm settlement, with sub-piconewton forces sufficient to prevent biofilm initiation. As a consequence, distinct hydrodynamic conditions, which set spatial distribution of shear stress, promoted distinct colonization patterns with consequences on the growth mode. However, no direct impact of mechanical forces on biofilm growth rate was observed. Consistently, no mechanosensing gene emerged from our differential transcriptomic analysis comparing distinct hydrodynamic conditions. Instead, we found that hydrodynamic molecular transport crucially impacts biofilm growth by controlling oxygen availability. Our results shed light on biofilm response to hydrodynamics and open new avenues to achieve informed design of fluidic setups for investigating, engineering or fighting adherent communities. |
| first_indexed | 2024-12-13T23:05:53Z |
| format | Article |
| id | doaj.art-09513a5520e54e14a7a1e8f2e600cdb5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T23:05:53Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-09513a5520e54e14a7a1e8f2e600cdb52022-12-21T23:28:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01124e017519710.1371/journal.pone.0175197Bacterial biofilm under flow: First a physical struggle to stay, then a matter of breathing.Philippe ThomenJérôme RobertAmaury MonmeyranAnne-Florence BitbolCarine DouarcheNelly HenryBacterial communities attached to surfaces under fluid flow represent a widespread lifestyle of the microbial world. Through shear stress generation and molecular transport regulation, hydrodynamics conveys effects that are very different by nature but strongly coupled. To decipher the influence of these levers on bacterial biofilms immersed in moving fluids, we quantitatively and simultaneously investigated physicochemical and biological properties of the biofilm. We designed a millifluidic setup allowing to control hydrodynamic conditions and to monitor biofilm development in real time using microscope imaging. We also conducted a transcriptomic analysis to detect a potential physiological response to hydrodynamics. We discovered that a threshold value of shear stress determined biofilm settlement, with sub-piconewton forces sufficient to prevent biofilm initiation. As a consequence, distinct hydrodynamic conditions, which set spatial distribution of shear stress, promoted distinct colonization patterns with consequences on the growth mode. However, no direct impact of mechanical forces on biofilm growth rate was observed. Consistently, no mechanosensing gene emerged from our differential transcriptomic analysis comparing distinct hydrodynamic conditions. Instead, we found that hydrodynamic molecular transport crucially impacts biofilm growth by controlling oxygen availability. Our results shed light on biofilm response to hydrodynamics and open new avenues to achieve informed design of fluidic setups for investigating, engineering or fighting adherent communities.http://europepmc.org/articles/PMC5389662?pdf=render |
| spellingShingle | Philippe Thomen Jérôme Robert Amaury Monmeyran Anne-Florence Bitbol Carine Douarche Nelly Henry Bacterial biofilm under flow: First a physical struggle to stay, then a matter of breathing. PLoS ONE |
| title | Bacterial biofilm under flow: First a physical struggle to stay, then a matter of breathing. |
| title_full | Bacterial biofilm under flow: First a physical struggle to stay, then a matter of breathing. |
| title_fullStr | Bacterial biofilm under flow: First a physical struggle to stay, then a matter of breathing. |
| title_full_unstemmed | Bacterial biofilm under flow: First a physical struggle to stay, then a matter of breathing. |
| title_short | Bacterial biofilm under flow: First a physical struggle to stay, then a matter of breathing. |
| title_sort | bacterial biofilm under flow first a physical struggle to stay then a matter of breathing |
| url | http://europepmc.org/articles/PMC5389662?pdf=render |
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