In vitro antimicrobial activity of Spinacia Oleracea against Streptococcus mutans and Lactobacillus acidophilus
Introduction: The use of traditional medicine is well known in India. The high number of bacterial resistance to antibiotics drives much of the current interest in natural antimicrobial molecules. Spinacia oleracea (spinach) possesses medicinal properties. Dental caries is prevalent in about 89% of...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Journal of Indian Association of Public Health Dentistry |
| Subjects: | |
| Online Access: | http://www.jiaphd.org/article.asp?issn=2319-5932;year=2018;volume=16;issue=3;spage=251;epage=255;aulast=Adapa |
| _version_ | 1811192949905555456 |
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| author | Suma Bindu Adapa V H Sushanth G M Prashant Imranula Mohamed |
| author_facet | Suma Bindu Adapa V H Sushanth G M Prashant Imranula Mohamed |
| author_sort | Suma Bindu Adapa |
| collection | DOAJ |
| description | Introduction: The use of traditional medicine is well known in India. The high number of bacterial resistance to antibiotics drives much of the current interest in natural antimicrobial molecules. Spinacia oleracea (spinach) possesses medicinal properties. Dental caries is prevalent in about 89% of the Indian population. Streptococcus mutans is the primary causative organism for dental caries and Lactobacillus for further development of caries. Aim: The aim of this study is to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of aqueous and ethanolic extracts of spinach against S. mutans and Lactobacillus. Materials and Methods: About 10 g of freshly harvested spinach was extracted with 95% ethanol and distilled water. The extract was converted into dried powder in desiccator and was dissolved to get 20 mg/ml concentration. The MIC was determined by nine dilutions of each aqueous and ethanolic extracts made with brain–heart infusion. To determine the MBC, the MIC dilution tubes, with no visible growth, and the control tube were subcultured onto the broth media and incubated for 24 h anaerobically and the colonies were counted on the next day. Results: The ethanolic extract exhibited MIC at 12.5 μg/ml against S. mutans and at 25 μg/ml against Lactobacillus. In MBC, the ethanolic extract showed greater bacteriostatic activity against S. mutans compared to Lactobacillus acidophilus. Conclusion: There is preliminary evidence for the antimicrobial activity of spinach extracts against S. mutans and this raises the possibility that spinach may have preventive effect on dental caries. |
| first_indexed | 2024-04-12T00:00:08Z |
| format | Article |
| id | doaj.art-0952712168c74971b8f7a78b538d3b4f |
| institution | Directory Open Access Journal |
| issn | 2319-5932 2350-0484 |
| language | English |
| last_indexed | 2024-04-12T00:00:08Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Indian Association of Public Health Dentistry |
| spelling | doaj.art-0952712168c74971b8f7a78b538d3b4f2022-12-22T03:56:15ZengWolters Kluwer Medknow PublicationsJournal of Indian Association of Public Health Dentistry2319-59322350-04842018-01-0116325125510.4103/jiaphd.jiaphd_6_18In vitro antimicrobial activity of Spinacia Oleracea against Streptococcus mutans and Lactobacillus acidophilusSuma Bindu AdapaV H SushanthG M PrashantImranula MohamedIntroduction: The use of traditional medicine is well known in India. The high number of bacterial resistance to antibiotics drives much of the current interest in natural antimicrobial molecules. Spinacia oleracea (spinach) possesses medicinal properties. Dental caries is prevalent in about 89% of the Indian population. Streptococcus mutans is the primary causative organism for dental caries and Lactobacillus for further development of caries. Aim: The aim of this study is to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of aqueous and ethanolic extracts of spinach against S. mutans and Lactobacillus. Materials and Methods: About 10 g of freshly harvested spinach was extracted with 95% ethanol and distilled water. The extract was converted into dried powder in desiccator and was dissolved to get 20 mg/ml concentration. The MIC was determined by nine dilutions of each aqueous and ethanolic extracts made with brain–heart infusion. To determine the MBC, the MIC dilution tubes, with no visible growth, and the control tube were subcultured onto the broth media and incubated for 24 h anaerobically and the colonies were counted on the next day. Results: The ethanolic extract exhibited MIC at 12.5 μg/ml against S. mutans and at 25 μg/ml against Lactobacillus. In MBC, the ethanolic extract showed greater bacteriostatic activity against S. mutans compared to Lactobacillus acidophilus. Conclusion: There is preliminary evidence for the antimicrobial activity of spinach extracts against S. mutans and this raises the possibility that spinach may have preventive effect on dental caries.http://www.jiaphd.org/article.asp?issn=2319-5932;year=2018;volume=16;issue=3;spage=251;epage=255;aulast=AdapaDental cariesLactobacillus acidophilusSpinacia oleraceaStreptococcus mutans |
| spellingShingle | Suma Bindu Adapa V H Sushanth G M Prashant Imranula Mohamed In vitro antimicrobial activity of Spinacia Oleracea against Streptococcus mutans and Lactobacillus acidophilus Journal of Indian Association of Public Health Dentistry Dental caries Lactobacillus acidophilus Spinacia oleracea Streptococcus mutans |
| title | In vitro antimicrobial activity of Spinacia Oleracea against Streptococcus mutans and Lactobacillus acidophilus |
| title_full | In vitro antimicrobial activity of Spinacia Oleracea against Streptococcus mutans and Lactobacillus acidophilus |
| title_fullStr | In vitro antimicrobial activity of Spinacia Oleracea against Streptococcus mutans and Lactobacillus acidophilus |
| title_full_unstemmed | In vitro antimicrobial activity of Spinacia Oleracea against Streptococcus mutans and Lactobacillus acidophilus |
| title_short | In vitro antimicrobial activity of Spinacia Oleracea against Streptococcus mutans and Lactobacillus acidophilus |
| title_sort | in vitro antimicrobial activity of spinacia oleracea against streptococcus mutans and lactobacillus acidophilus |
| topic | Dental caries Lactobacillus acidophilus Spinacia oleracea Streptococcus mutans |
| url | http://www.jiaphd.org/article.asp?issn=2319-5932;year=2018;volume=16;issue=3;spage=251;epage=255;aulast=Adapa |
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