Neural differentiation of choroid plexus epithelial cells: role of human traumatic cerebrospinal fluid
As the key producer of cerebrospinal fluid (CSF), the choroid plexus (CP) provides a unique protective system in the central nervous system. CSF components are not invariable and they can change based on the pathological conditions of the central nervous system. The purpose of the present study was...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=1;spage=84;epage=89;aulast=Hashemi |
| _version_ | 1818894493409083392 |
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| author | Elham Hashemi Yousef Sadeghi Abbas Aliaghaei Afsoun Seddighi Abbas Piryaei Mehdi Eskandarian Broujeni Fatemeh Shaerzadeh Abdollah Amini Ramin Pouriran |
| author_facet | Elham Hashemi Yousef Sadeghi Abbas Aliaghaei Afsoun Seddighi Abbas Piryaei Mehdi Eskandarian Broujeni Fatemeh Shaerzadeh Abdollah Amini Ramin Pouriran |
| author_sort | Elham Hashemi |
| collection | DOAJ |
| description | As the key producer of cerebrospinal fluid (CSF), the choroid plexus (CP) provides a unique protective system in the central nervous system. CSF components are not invariable and they can change based on the pathological conditions of the central nervous system. The purpose of the present study was to assess the effects of non-traumatic and traumatic CSF on the differentiation of multipotent stem-like cells of CP into the neural and/or glial cells. CP epithelial cells were isolated from adult male rats and treated with human non-traumatic and traumatic CSF. Alterations in mRNA expression of Nestin and microtubule-associated protein (MAP2), as the specific markers of neurogenesis, and astrocyte marker glial fibrillary acidic protein (GFAP) in cultured CP epithelial cells were evaluated using quantitative real-time PCR. The data revealed that treatment with CSF (non-traumatic and traumatic) led to increase in mRNA expression levels of MAP2 and GFAP. Moreover, the expression of Nestin decreased in CP epithelial cells treated with non-traumatic CSF, while treatment with traumatic CSF significantly increased its mRNA level compared to the cells cultured only in DMEM/F12 as control. It seems that CP epithelial cells contain multipotent stem-like cells which are inducible under pathological conditions including exposure to traumatic CSF because of its compositions. |
| first_indexed | 2024-12-19T18:29:22Z |
| format | Article |
| id | doaj.art-0953338f97e84e48b3832456ec3cf31c |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-19T18:29:22Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-0953338f97e84e48b3832456ec3cf31c2022-12-21T20:10:46ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742017-01-01121848910.4103/1673-5374.198989Neural differentiation of choroid plexus epithelial cells: role of human traumatic cerebrospinal fluidElham HashemiYousef SadeghiAbbas AliaghaeiAfsoun SeddighiAbbas PiryaeiMehdi Eskandarian BroujeniFatemeh ShaerzadehAbdollah AminiRamin PouriranAs the key producer of cerebrospinal fluid (CSF), the choroid plexus (CP) provides a unique protective system in the central nervous system. CSF components are not invariable and they can change based on the pathological conditions of the central nervous system. The purpose of the present study was to assess the effects of non-traumatic and traumatic CSF on the differentiation of multipotent stem-like cells of CP into the neural and/or glial cells. CP epithelial cells were isolated from adult male rats and treated with human non-traumatic and traumatic CSF. Alterations in mRNA expression of Nestin and microtubule-associated protein (MAP2), as the specific markers of neurogenesis, and astrocyte marker glial fibrillary acidic protein (GFAP) in cultured CP epithelial cells were evaluated using quantitative real-time PCR. The data revealed that treatment with CSF (non-traumatic and traumatic) led to increase in mRNA expression levels of MAP2 and GFAP. Moreover, the expression of Nestin decreased in CP epithelial cells treated with non-traumatic CSF, while treatment with traumatic CSF significantly increased its mRNA level compared to the cells cultured only in DMEM/F12 as control. It seems that CP epithelial cells contain multipotent stem-like cells which are inducible under pathological conditions including exposure to traumatic CSF because of its compositions.http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=1;spage=84;epage=89;aulast=Hasheminerve regeneration; choroid plexus; cerebrospinal fluid; stem cells; Nestin; microtubule-associated protein 2; glial fibrillary acidic protein; neurogenesis; central nervous system; neural regeneration |
| spellingShingle | Elham Hashemi Yousef Sadeghi Abbas Aliaghaei Afsoun Seddighi Abbas Piryaei Mehdi Eskandarian Broujeni Fatemeh Shaerzadeh Abdollah Amini Ramin Pouriran Neural differentiation of choroid plexus epithelial cells: role of human traumatic cerebrospinal fluid Neural Regeneration Research nerve regeneration; choroid plexus; cerebrospinal fluid; stem cells; Nestin; microtubule-associated protein 2; glial fibrillary acidic protein; neurogenesis; central nervous system; neural regeneration |
| title | Neural differentiation of choroid plexus epithelial cells: role of human traumatic cerebrospinal fluid |
| title_full | Neural differentiation of choroid plexus epithelial cells: role of human traumatic cerebrospinal fluid |
| title_fullStr | Neural differentiation of choroid plexus epithelial cells: role of human traumatic cerebrospinal fluid |
| title_full_unstemmed | Neural differentiation of choroid plexus epithelial cells: role of human traumatic cerebrospinal fluid |
| title_short | Neural differentiation of choroid plexus epithelial cells: role of human traumatic cerebrospinal fluid |
| title_sort | neural differentiation of choroid plexus epithelial cells role of human traumatic cerebrospinal fluid |
| topic | nerve regeneration; choroid plexus; cerebrospinal fluid; stem cells; Nestin; microtubule-associated protein 2; glial fibrillary acidic protein; neurogenesis; central nervous system; neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=1;spage=84;epage=89;aulast=Hashemi |
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