Identification of short-chain oxidized phosphatidylcholine in human plasma
Oxidized phospholipids have been recognized as potentially important compounds that carry biological activities similar to the platelet-activating factor, but their presence in biological tissue has not been firmly established. We developed a novel technique for the quantitative analysis of phosphol...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1996-12-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520374642 |
| _version_ | 1818675649343127552 |
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| author | M Schlame R Haupt I Wiswedel W J Kox B Rüstow |
| author_facet | M Schlame R Haupt I Wiswedel W J Kox B Rüstow |
| author_sort | M Schlame |
| collection | DOAJ |
| description | Oxidized phospholipids have been recognized as potentially important compounds that carry biological activities similar to the platelet-activating factor, but their presence in biological tissue has not been firmly established. We developed a novel technique for the quantitative analysis of phospholipids with oxidized acyl chains. The method involves 1) lipid extraction, 2) chromatographic enrichment of phospholipids with short acyl chains, 3) derivatization with 9-(chloromethyl)anthracene, 4) solid-phase extraction of the derivatives, and 5) reversed-phase HPLC with fluorescence detection. The technique was capable of measuring dicarboxylate-containing phosphatidylcholines (PCs) at the picomole level. The method was suited to monitor the generation of oxidized phospholipids from 1-palmitoyl-2-arachidonoyl-PC in the presence of Fe21/ascorbate. The new procedure was used to isolate lipids from human plasma that were identified as anthracene derivatives of short-chain oxidized PC on the basis of chromatographic enzymatic, and spectroscopic evidence. The plasma concentration, determined with an internal standard (1-palmitoyl-2-suberoyl-PC), was 0.6 +/- 0.2 microM (n = 11). The analytical method did not produce oxidation antifacts in significant amount. We concluded that human blood contains oxidatively fragmented PC in submicromolar concentration. |
| first_indexed | 2024-12-17T08:30:56Z |
| format | Article |
| id | doaj.art-0954ecae1bce47e5b1ff0c55c67cb0ae |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T08:30:56Z |
| publishDate | 1996-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-0954ecae1bce47e5b1ff0c55c67cb0ae2022-12-21T21:56:36ZengElsevierJournal of Lipid Research0022-22751996-12-01371226082615Identification of short-chain oxidized phosphatidylcholine in human plasmaM Schlame0R Haupt1I Wiswedel2W J Kox3B Rüstow4Department of Anesthesiology and Intensive Therapy, University Hospital Charité, Humboldt University, Berlin, Germany.Department of Anesthesiology and Intensive Therapy, University Hospital Charité, Humboldt University, Berlin, Germany.Department of Anesthesiology and Intensive Therapy, University Hospital Charité, Humboldt University, Berlin, Germany.Department of Anesthesiology and Intensive Therapy, University Hospital Charité, Humboldt University, Berlin, Germany.Department of Anesthesiology and Intensive Therapy, University Hospital Charité, Humboldt University, Berlin, Germany.Oxidized phospholipids have been recognized as potentially important compounds that carry biological activities similar to the platelet-activating factor, but their presence in biological tissue has not been firmly established. We developed a novel technique for the quantitative analysis of phospholipids with oxidized acyl chains. The method involves 1) lipid extraction, 2) chromatographic enrichment of phospholipids with short acyl chains, 3) derivatization with 9-(chloromethyl)anthracene, 4) solid-phase extraction of the derivatives, and 5) reversed-phase HPLC with fluorescence detection. The technique was capable of measuring dicarboxylate-containing phosphatidylcholines (PCs) at the picomole level. The method was suited to monitor the generation of oxidized phospholipids from 1-palmitoyl-2-arachidonoyl-PC in the presence of Fe21/ascorbate. The new procedure was used to isolate lipids from human plasma that were identified as anthracene derivatives of short-chain oxidized PC on the basis of chromatographic enzymatic, and spectroscopic evidence. The plasma concentration, determined with an internal standard (1-palmitoyl-2-suberoyl-PC), was 0.6 +/- 0.2 microM (n = 11). The analytical method did not produce oxidation antifacts in significant amount. We concluded that human blood contains oxidatively fragmented PC in submicromolar concentration.http://www.sciencedirect.com/science/article/pii/S0022227520374642 |
| spellingShingle | M Schlame R Haupt I Wiswedel W J Kox B Rüstow Identification of short-chain oxidized phosphatidylcholine in human plasma Journal of Lipid Research |
| title | Identification of short-chain oxidized phosphatidylcholine in human plasma |
| title_full | Identification of short-chain oxidized phosphatidylcholine in human plasma |
| title_fullStr | Identification of short-chain oxidized phosphatidylcholine in human plasma |
| title_full_unstemmed | Identification of short-chain oxidized phosphatidylcholine in human plasma |
| title_short | Identification of short-chain oxidized phosphatidylcholine in human plasma |
| title_sort | identification of short chain oxidized phosphatidylcholine in human plasma |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520374642 |
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