Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens
<p>Abstract</p> <p>Background</p> <p>We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.</p> <p>Methods</p...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2003-06-01
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| Series: | BMC Infectious Diseases |
| Online Access: | http://www.biomedcentral.com/1471-2334/3/12 |
| Summary: | <p>Abstract</p> <p>Background</p> <p>We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.</p> <p>Methods</p> <p>Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes.</p> <p>Results</p> <p>Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples.</p> <p>Conclusions</p> <p>This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.</p> |
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| ISSN: | 1471-2334 |