Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens
<p>Abstract</p> <p>Background</p> <p>We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.</p> <p>Methods</p...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2003-06-01
|
| Series: | BMC Infectious Diseases |
| Online Access: | http://www.biomedcentral.com/1471-2334/3/12 |
| _version_ | 1818759601689985024 |
|---|---|
| author | Miller Donna L Chan Vivian Unger Elizabeth R Farkas Daniel H Vernon Suzanne D Chen Yin-Peng Blackburn Gary F Reeves William C |
| author_facet | Miller Donna L Chan Vivian Unger Elizabeth R Farkas Daniel H Vernon Suzanne D Chen Yin-Peng Blackburn Gary F Reeves William C |
| author_sort | Miller Donna L |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.</p> <p>Methods</p> <p>Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes.</p> <p>Results</p> <p>Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples.</p> <p>Conclusions</p> <p>This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.</p> |
| first_indexed | 2024-12-18T06:45:19Z |
| format | Article |
| id | doaj.art-0955e63e9a044114956b69899f928ff0 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-18T06:45:19Z |
| publishDate | 2003-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-0955e63e9a044114956b69899f928ff02022-12-21T21:17:30ZengBMCBMC Infectious Diseases1471-23342003-06-01311210.1186/1471-2334-3-12Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogensMiller Donna LChan VivianUnger Elizabeth RFarkas Daniel HVernon Suzanne DChen Yin-PengBlackburn Gary FReeves William C<p>Abstract</p> <p>Background</p> <p>We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.</p> <p>Methods</p> <p>Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes.</p> <p>Results</p> <p>Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples.</p> <p>Conclusions</p> <p>This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.</p>http://www.biomedcentral.com/1471-2334/3/12 |
| spellingShingle | Miller Donna L Chan Vivian Unger Elizabeth R Farkas Daniel H Vernon Suzanne D Chen Yin-Peng Blackburn Gary F Reeves William C Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens BMC Infectious Diseases |
| title | Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens |
| title_full | Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens |
| title_fullStr | Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens |
| title_full_unstemmed | Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens |
| title_short | Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens |
| title_sort | bioelectronic dna detection of human papillomaviruses using esensor™ a model system for detection of multiple pathogens |
| url | http://www.biomedcentral.com/1471-2334/3/12 |
| work_keys_str_mv | AT millerdonnal bioelectronicdnadetectionofhumanpapillomavirusesusingesensoramodelsystemfordetectionofmultiplepathogens AT chanvivian bioelectronicdnadetectionofhumanpapillomavirusesusingesensoramodelsystemfordetectionofmultiplepathogens AT ungerelizabethr bioelectronicdnadetectionofhumanpapillomavirusesusingesensoramodelsystemfordetectionofmultiplepathogens AT farkasdanielh bioelectronicdnadetectionofhumanpapillomavirusesusingesensoramodelsystemfordetectionofmultiplepathogens AT vernonsuzanned bioelectronicdnadetectionofhumanpapillomavirusesusingesensoramodelsystemfordetectionofmultiplepathogens AT chenyinpeng bioelectronicdnadetectionofhumanpapillomavirusesusingesensoramodelsystemfordetectionofmultiplepathogens AT blackburngaryf bioelectronicdnadetectionofhumanpapillomavirusesusingesensoramodelsystemfordetectionofmultiplepathogens AT reeveswilliamc bioelectronicdnadetectionofhumanpapillomavirusesusingesensoramodelsystemfordetectionofmultiplepathogens |