Antibacterial Mechanism of <i>Dellaglioa algida</i> against <i>Pseudomonas fluorescens</i> and <i>Pseudomonas fragi</i>
<i>Pseudomonas fluorescens (P. fluorescens)</i> and <i>Pseudomonas fragi (P. fragi)</i>, two kinds of psychrotrophic <i>Pseudomonas</i> species with pathogenicity, are likely to contaminate foods and cause diseases even in fairly cold environments, an outcome whic...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/8/7/298 |
| _version_ | 1797433574288261120 |
|---|---|
| author | Yao Sun Shiyu Liu Zhe Zhan Tianhui Wei Tongqing Ma Jie Sun Jinzhu Song |
| author_facet | Yao Sun Shiyu Liu Zhe Zhan Tianhui Wei Tongqing Ma Jie Sun Jinzhu Song |
| author_sort | Yao Sun |
| collection | DOAJ |
| description | <i>Pseudomonas fluorescens (P. fluorescens)</i> and <i>Pseudomonas fragi (P. fragi)</i>, two kinds of psychrotrophic <i>Pseudomonas</i> species with pathogenicity, are likely to contaminate foods and cause diseases even in fairly cold environments, an outcome which should be suppressed. This paper investigates the antibacterial mechanisms of <i>Dellaglioa algida (D. algida)</i>, a new type of low-temperature-resistant <i>Lactobacillus</i>, on two such <i>Pseudomonas</i>. By the enzyme treatment approach, the antibacterial substance existing in the cell-free supernatant (CFS) of <i>D. algida</i> is preliminarily determined as organic acid or protein; then, its inhibition effects are assessed under various culture environments, including pH value, salinity, and culture time, where the best antibacterial performance is achieved at pH = 6.00, S = 0%, and culture time = 48 h. A series of experiments on biofilms indicate that <i>D. algida</i> is not only able to inhibit the generation or damage the integrality of the biofilm of the two mentioned <i>Pseudomonas</i>, but also can reduce the motility, including swarming and swimming, of <i>P. fragi</i> and restrain the swarming of <i>P. fluorescens</i>. The aformentioned developed antibacterial mechanisms show the possibility of using <i>D. algida</i> in applications as an inhibitor for psychrotrophic <i>Pseudomonas</i> in the food industry, by virtue of its strong suppression capability, especially in cold environments. |
| first_indexed | 2024-03-09T10:18:55Z |
| format | Article |
| id | doaj.art-09560004b8e541d0a8adff43b72f7aca |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T10:18:55Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-09560004b8e541d0a8adff43b72f7aca2023-12-01T22:08:09ZengMDPI AGFermentation2311-56372022-06-018729810.3390/fermentation8070298Antibacterial Mechanism of <i>Dellaglioa algida</i> against <i>Pseudomonas fluorescens</i> and <i>Pseudomonas fragi</i>Yao Sun0Shiyu Liu1Zhe Zhan2Tianhui Wei3Tongqing Ma4Jie Sun5Jinzhu Song6School of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, ChinaSchool of Life Science and Technology, Harbin Institute of Technology, Harbin 150000, China<i>Pseudomonas fluorescens (P. fluorescens)</i> and <i>Pseudomonas fragi (P. fragi)</i>, two kinds of psychrotrophic <i>Pseudomonas</i> species with pathogenicity, are likely to contaminate foods and cause diseases even in fairly cold environments, an outcome which should be suppressed. This paper investigates the antibacterial mechanisms of <i>Dellaglioa algida (D. algida)</i>, a new type of low-temperature-resistant <i>Lactobacillus</i>, on two such <i>Pseudomonas</i>. By the enzyme treatment approach, the antibacterial substance existing in the cell-free supernatant (CFS) of <i>D. algida</i> is preliminarily determined as organic acid or protein; then, its inhibition effects are assessed under various culture environments, including pH value, salinity, and culture time, where the best antibacterial performance is achieved at pH = 6.00, S = 0%, and culture time = 48 h. A series of experiments on biofilms indicate that <i>D. algida</i> is not only able to inhibit the generation or damage the integrality of the biofilm of the two mentioned <i>Pseudomonas</i>, but also can reduce the motility, including swarming and swimming, of <i>P. fragi</i> and restrain the swarming of <i>P. fluorescens</i>. The aformentioned developed antibacterial mechanisms show the possibility of using <i>D. algida</i> in applications as an inhibitor for psychrotrophic <i>Pseudomonas</i> in the food industry, by virtue of its strong suppression capability, especially in cold environments.https://www.mdpi.com/2311-5637/8/7/298<i>Dellaglioa algida</i><i>Lactobacillus</i>psychrotrophic <i>Pseudomonas</i>antibacterial mechanism |
| spellingShingle | Yao Sun Shiyu Liu Zhe Zhan Tianhui Wei Tongqing Ma Jie Sun Jinzhu Song Antibacterial Mechanism of <i>Dellaglioa algida</i> against <i>Pseudomonas fluorescens</i> and <i>Pseudomonas fragi</i> Fermentation <i>Dellaglioa algida</i> <i>Lactobacillus</i> psychrotrophic <i>Pseudomonas</i> antibacterial mechanism |
| title | Antibacterial Mechanism of <i>Dellaglioa algida</i> against <i>Pseudomonas fluorescens</i> and <i>Pseudomonas fragi</i> |
| title_full | Antibacterial Mechanism of <i>Dellaglioa algida</i> against <i>Pseudomonas fluorescens</i> and <i>Pseudomonas fragi</i> |
| title_fullStr | Antibacterial Mechanism of <i>Dellaglioa algida</i> against <i>Pseudomonas fluorescens</i> and <i>Pseudomonas fragi</i> |
| title_full_unstemmed | Antibacterial Mechanism of <i>Dellaglioa algida</i> against <i>Pseudomonas fluorescens</i> and <i>Pseudomonas fragi</i> |
| title_short | Antibacterial Mechanism of <i>Dellaglioa algida</i> against <i>Pseudomonas fluorescens</i> and <i>Pseudomonas fragi</i> |
| title_sort | antibacterial mechanism of i dellaglioa algida i against i pseudomonas fluorescens i and i pseudomonas fragi i |
| topic | <i>Dellaglioa algida</i> <i>Lactobacillus</i> psychrotrophic <i>Pseudomonas</i> antibacterial mechanism |
| url | https://www.mdpi.com/2311-5637/8/7/298 |
| work_keys_str_mv | AT yaosun antibacterialmechanismofidellaglioaalgidaiagainstipseudomonasfluorescensiandipseudomonasfragii AT shiyuliu antibacterialmechanismofidellaglioaalgidaiagainstipseudomonasfluorescensiandipseudomonasfragii AT zhezhan antibacterialmechanismofidellaglioaalgidaiagainstipseudomonasfluorescensiandipseudomonasfragii AT tianhuiwei antibacterialmechanismofidellaglioaalgidaiagainstipseudomonasfluorescensiandipseudomonasfragii AT tongqingma antibacterialmechanismofidellaglioaalgidaiagainstipseudomonasfluorescensiandipseudomonasfragii AT jiesun antibacterialmechanismofidellaglioaalgidaiagainstipseudomonasfluorescensiandipseudomonasfragii AT jinzhusong antibacterialmechanismofidellaglioaalgidaiagainstipseudomonasfluorescensiandipseudomonasfragii |