ARC/Arg3.1 expression in the lateral geniculate body of monocular form deprivation amblyopic kittens
Abstract Purpose The present study compared the expression of activity-regulated cytoskeleton-associated protein (ARC/Arg3.1) in the lateral geniculate body between form deprivation amblyopia kittens and normal kittens to examine the significance of ARC/Arg3.1 in the lateral geniculate body in the p...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | BMC Ophthalmology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12886-022-02757-5 |
| _version_ | 1797958687595167744 |
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| author | Haobo Fan Ying Wang Yunchun Zou Weiqi Song Juan Xie Xiuping Tang Siyu Chen |
| author_facet | Haobo Fan Ying Wang Yunchun Zou Weiqi Song Juan Xie Xiuping Tang Siyu Chen |
| author_sort | Haobo Fan |
| collection | DOAJ |
| description | Abstract Purpose The present study compared the expression of activity-regulated cytoskeleton-associated protein (ARC/Arg3.1) in the lateral geniculate body between form deprivation amblyopia kittens and normal kittens to examine the significance of ARC/Arg3.1 in the lateral geniculate body in the pathogenesis of amblyopia. Methods Twenty kittens were randomly divided into an experimental group (n = 10) and a control group (n = 10). Black opaque covering cloth was used to cover the right eye of kittens in the experimental group. Pattern visual evoked potentials (PVEP) were detected weekly in all kittens. The expression of the ARC/Arg3.1 gene was detected by immunohistochemistry and in situ hybridization, and apoptosis of lateral geniculate body cells was detected by TUNEL. Results PVEP detection showed that at the age of 5 and 7 weeks, the latency of P100 in the right eye of the experimental group was higher than that of the other three groups (P < 0.05), and the amplitude of P100 was lower than that of the other three groups (P < 0.05). The expression of ARC/Arg3.1 protein (P < 0.05) and mRNA (P < 0.05) in the lateral geniculate body of the experimental group was significantly lower than that of the control group. The level of neuronal apoptosis in the experimental group was higher than that in the control group (P < 0.05). The expression of the ARC/Arg3.1 gene was negatively correlated with the apoptosis level of lateral geniculate body neurons. Conclusions The expression of ARC/Arg3.1 is associated with monocular form deprivation amblyopia and apoptosis of lateral geniculate body cells. |
| first_indexed | 2024-04-11T00:23:37Z |
| format | Article |
| id | doaj.art-095b6cbb073c46888c3298573e2ea312 |
| institution | Directory Open Access Journal |
| issn | 1471-2415 |
| language | English |
| last_indexed | 2024-04-11T00:23:37Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Ophthalmology |
| spelling | doaj.art-095b6cbb073c46888c3298573e2ea3122023-01-08T12:07:28ZengBMCBMC Ophthalmology1471-24152023-01-0123111010.1186/s12886-022-02757-5ARC/Arg3.1 expression in the lateral geniculate body of monocular form deprivation amblyopic kittensHaobo Fan0Ying Wang1Yunchun Zou2Weiqi Song3Juan Xie4Xiuping Tang5Siyu Chen6Department of Optometry, North Sichuan Medical CollegeDepartment of Optometry, North Sichuan Medical CollegeDepartment of Optometry, North Sichuan Medical CollegeDepartment of Optometry, North Sichuan Medical CollegeDepartment of Optometry, North Sichuan Medical CollegeDepartment of Optometry, North Sichuan Medical CollegeDepartment of Optometry, North Sichuan Medical CollegeAbstract Purpose The present study compared the expression of activity-regulated cytoskeleton-associated protein (ARC/Arg3.1) in the lateral geniculate body between form deprivation amblyopia kittens and normal kittens to examine the significance of ARC/Arg3.1 in the lateral geniculate body in the pathogenesis of amblyopia. Methods Twenty kittens were randomly divided into an experimental group (n = 10) and a control group (n = 10). Black opaque covering cloth was used to cover the right eye of kittens in the experimental group. Pattern visual evoked potentials (PVEP) were detected weekly in all kittens. The expression of the ARC/Arg3.1 gene was detected by immunohistochemistry and in situ hybridization, and apoptosis of lateral geniculate body cells was detected by TUNEL. Results PVEP detection showed that at the age of 5 and 7 weeks, the latency of P100 in the right eye of the experimental group was higher than that of the other three groups (P < 0.05), and the amplitude of P100 was lower than that of the other three groups (P < 0.05). The expression of ARC/Arg3.1 protein (P < 0.05) and mRNA (P < 0.05) in the lateral geniculate body of the experimental group was significantly lower than that of the control group. The level of neuronal apoptosis in the experimental group was higher than that in the control group (P < 0.05). The expression of the ARC/Arg3.1 gene was negatively correlated with the apoptosis level of lateral geniculate body neurons. Conclusions The expression of ARC/Arg3.1 is associated with monocular form deprivation amblyopia and apoptosis of lateral geniculate body cells.https://doi.org/10.1186/s12886-022-02757-5AmblyopiaActivity-regulated cytoskeleton-associated (ARC/Arg3.1)Lateral geniculate bodyForm deprivation |
| spellingShingle | Haobo Fan Ying Wang Yunchun Zou Weiqi Song Juan Xie Xiuping Tang Siyu Chen ARC/Arg3.1 expression in the lateral geniculate body of monocular form deprivation amblyopic kittens BMC Ophthalmology Amblyopia Activity-regulated cytoskeleton-associated (ARC/Arg3.1) Lateral geniculate body Form deprivation |
| title | ARC/Arg3.1 expression in the lateral geniculate body of monocular form deprivation amblyopic kittens |
| title_full | ARC/Arg3.1 expression in the lateral geniculate body of monocular form deprivation amblyopic kittens |
| title_fullStr | ARC/Arg3.1 expression in the lateral geniculate body of monocular form deprivation amblyopic kittens |
| title_full_unstemmed | ARC/Arg3.1 expression in the lateral geniculate body of monocular form deprivation amblyopic kittens |
| title_short | ARC/Arg3.1 expression in the lateral geniculate body of monocular form deprivation amblyopic kittens |
| title_sort | arc arg3 1 expression in the lateral geniculate body of monocular form deprivation amblyopic kittens |
| topic | Amblyopia Activity-regulated cytoskeleton-associated (ARC/Arg3.1) Lateral geniculate body Form deprivation |
| url | https://doi.org/10.1186/s12886-022-02757-5 |
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