Evaluation of the Phytochemical and Antimicrobial potential of the Leaf Extracts of <i>Bryophyllum pinnatum</i> L.and <i>Citrus aurantifolia</i> Sw. and their Synergy
The aim of this research is to comparatively study the phytochemical and antimicrobial properties of Bryophyllum pinnatum and Citrus aurantifolia leaf extracts and their synergy. Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa, Aspergillus niger, Mucor muc...
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| Format: | Article |
| Language: | English |
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Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)
2016-02-01
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| Series: | Journal of Applied Sciences and Environmental Management |
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| Online Access: | https://www.ajol.info/index.php/jasem/article/view/131201 |
| _version_ | 1797227875088203776 |
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| author | FI Akinnibosun O Edionwe |
| author_facet | FI Akinnibosun O Edionwe |
| author_sort | FI Akinnibosun |
| collection | DOAJ |
| description |
The aim of this research is to comparatively study the phytochemical and antimicrobial properties of Bryophyllum pinnatum and Citrus aurantifolia leaf extracts and their synergy. Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa, Aspergillus niger, Mucor mucedo, Penicillium notatum and Candida albicans, were used as test organisms and the antimicrobial activity of the extracts was determined by the agar-well diffusion method. Synergistic antibacterial activity ranged from 0.0 ± 0.0 to 2.4 ± 0.6, 11.3 ± 0.9 to 23.5 ± 1.1, 16.7 ± 0.3 to 27.3 ± 0.6 and 8.7 ± 0.9 to 22.7 ± 0.9, for aqueous, ethanol, methanol and acetone extracts respectively. Extracts of C. aurantifolia were more effective against the test organisms than B. pinnatum extracts, except the aqueous extract. Synergistic antifungal activity of the aqueous extract was 0.0 ± 0.0 mm for all the test fungi, the synergistic antifungal activity ranged from 8.7 ± 0.6 mm to 14.0 ± 0.9 mm, 10.0 ± 0.9 mm to 21.7 ± 0.6 mm and 0.0 ± 0.0 mm to 20.0 ± 0.6 mm for the ethanol, methanol and acetone extract respectively. Larger zones of inhibition were observed in the methanol extract of the synergy than the other extracting solvents. The synergy gave higher zones of inhibition neither B. pinnatum extract nor C. aurantifolia extract could give. It was also observed that the extracts compared well with the standard antimicrobial agents used as positive control. The phytochemical analysis of the extract revealed the presence of phytochemical constituents which conferred antimicrobial property on the plants. From the foregoing, the methanol extract of the synergy is considered the most effective in the treatment of infections caused by the test organisms.
Keywords: Bryophyllum pinnatum, Citrus aurantifolia, Synergy, Antimicrobial, Phytochemical constituents
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| first_indexed | 2024-04-24T14:47:45Z |
| format | Article |
| id | doaj.art-095ba8a61b5040dfa57a62ae39317c76 |
| institution | Directory Open Access Journal |
| issn | 2659-1502 2659-1499 |
| language | English |
| last_indexed | 2024-04-24T14:47:45Z |
| publishDate | 2016-02-01 |
| publisher | Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) |
| record_format | Article |
| series | Journal of Applied Sciences and Environmental Management |
| spelling | doaj.art-095ba8a61b5040dfa57a62ae39317c762024-04-02T19:53:11ZengJoint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)Journal of Applied Sciences and Environmental Management2659-15022659-14992016-02-0119410.4314/jasem.v19i4.7Evaluation of the Phytochemical and Antimicrobial potential of the Leaf Extracts of <i>Bryophyllum pinnatum</i> L.and <i>Citrus aurantifolia</i> Sw. and their SynergyFI AkinnibosunO Edionwe The aim of this research is to comparatively study the phytochemical and antimicrobial properties of Bryophyllum pinnatum and Citrus aurantifolia leaf extracts and their synergy. Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa, Aspergillus niger, Mucor mucedo, Penicillium notatum and Candida albicans, were used as test organisms and the antimicrobial activity of the extracts was determined by the agar-well diffusion method. Synergistic antibacterial activity ranged from 0.0 ± 0.0 to 2.4 ± 0.6, 11.3 ± 0.9 to 23.5 ± 1.1, 16.7 ± 0.3 to 27.3 ± 0.6 and 8.7 ± 0.9 to 22.7 ± 0.9, for aqueous, ethanol, methanol and acetone extracts respectively. Extracts of C. aurantifolia were more effective against the test organisms than B. pinnatum extracts, except the aqueous extract. Synergistic antifungal activity of the aqueous extract was 0.0 ± 0.0 mm for all the test fungi, the synergistic antifungal activity ranged from 8.7 ± 0.6 mm to 14.0 ± 0.9 mm, 10.0 ± 0.9 mm to 21.7 ± 0.6 mm and 0.0 ± 0.0 mm to 20.0 ± 0.6 mm for the ethanol, methanol and acetone extract respectively. Larger zones of inhibition were observed in the methanol extract of the synergy than the other extracting solvents. The synergy gave higher zones of inhibition neither B. pinnatum extract nor C. aurantifolia extract could give. It was also observed that the extracts compared well with the standard antimicrobial agents used as positive control. The phytochemical analysis of the extract revealed the presence of phytochemical constituents which conferred antimicrobial property on the plants. From the foregoing, the methanol extract of the synergy is considered the most effective in the treatment of infections caused by the test organisms. Keywords: Bryophyllum pinnatum, Citrus aurantifolia, Synergy, Antimicrobial, Phytochemical constituents https://www.ajol.info/index.php/jasem/article/view/131201Bryophyllum pinnatumCitrus aurantifoliaSynergyAntimicrobialPhytochemical constituents |
| spellingShingle | FI Akinnibosun O Edionwe Evaluation of the Phytochemical and Antimicrobial potential of the Leaf Extracts of <i>Bryophyllum pinnatum</i> L.and <i>Citrus aurantifolia</i> Sw. and their Synergy Journal of Applied Sciences and Environmental Management Bryophyllum pinnatum Citrus aurantifolia Synergy Antimicrobial Phytochemical constituents |
| title | Evaluation of the Phytochemical and Antimicrobial potential of the Leaf Extracts of <i>Bryophyllum pinnatum</i> L.and <i>Citrus aurantifolia</i> Sw. and their Synergy |
| title_full | Evaluation of the Phytochemical and Antimicrobial potential of the Leaf Extracts of <i>Bryophyllum pinnatum</i> L.and <i>Citrus aurantifolia</i> Sw. and their Synergy |
| title_fullStr | Evaluation of the Phytochemical and Antimicrobial potential of the Leaf Extracts of <i>Bryophyllum pinnatum</i> L.and <i>Citrus aurantifolia</i> Sw. and their Synergy |
| title_full_unstemmed | Evaluation of the Phytochemical and Antimicrobial potential of the Leaf Extracts of <i>Bryophyllum pinnatum</i> L.and <i>Citrus aurantifolia</i> Sw. and their Synergy |
| title_short | Evaluation of the Phytochemical and Antimicrobial potential of the Leaf Extracts of <i>Bryophyllum pinnatum</i> L.and <i>Citrus aurantifolia</i> Sw. and their Synergy |
| title_sort | evaluation of the phytochemical and antimicrobial potential of the leaf extracts of i bryophyllum pinnatum i l and i citrus aurantifolia i sw and their synergy |
| topic | Bryophyllum pinnatum Citrus aurantifolia Synergy Antimicrobial Phytochemical constituents |
| url | https://www.ajol.info/index.php/jasem/article/view/131201 |
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