Effect of Lipopolysaccharide and Muramyl Dipeptide on Apoptosis of Bovine Mammary Gland Lymphocytes
The aim of this study was to evaluate whether apoptosis of lymphocytes is modulated by stimulation by lipopolysaccharide (LPS) of <i>Escherichia coli</i> or muramyl dipeptide (MDP). Cell populations were obtained by lavaging of the mammary glands 24, 48, 72, and 168 h following intramamm...
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-06-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/10/6/990 |
| _version_ | 1797565990080348160 |
|---|---|
| author | Petr Slama Eliska Kabourkova Zbysek Sladek Terezie Zavadilova Lucie Kratochvilova Kristina Kharkevich Shubhadeep Roychoudhury Ales Pavlik Andrea Roztocilova Michal Uhrincat Vladimir Tancin Kazuhiro Kimura Roman Konecny Yoshio Kiku Atsushi Watanabe Jong-Young Kwak Monika Zouharova |
| author_facet | Petr Slama Eliska Kabourkova Zbysek Sladek Terezie Zavadilova Lucie Kratochvilova Kristina Kharkevich Shubhadeep Roychoudhury Ales Pavlik Andrea Roztocilova Michal Uhrincat Vladimir Tancin Kazuhiro Kimura Roman Konecny Yoshio Kiku Atsushi Watanabe Jong-Young Kwak Monika Zouharova |
| author_sort | Petr Slama |
| collection | DOAJ |
| description | The aim of this study was to evaluate whether apoptosis of lymphocytes is modulated by stimulation by lipopolysaccharide (LPS) of <i>Escherichia coli</i> or muramyl dipeptide (MDP). Cell populations were obtained by lavaging of the mammary glands 24, 48, 72, and 168 h following intramammary induced inflammation. The portion of apoptotic lymphocytes peaked at 48 h after treatment with LPS or MDP. The analysis of CD44 expression of the same cell populations showed a higher percentage of CD44-positive lymphocytes 24- and 48-h following induction of inflammation by LPS or MDP. The results demonstrate that during both experimental infection of bovine mammary glands with LPS or MDP, apoptosis of lymphocytes was induced in the initial phase of the inflammatory response and CD44 was also overexpressed at the beginning of inflammation. These data suggest a connection of lymphocyte apoptosis with the expression of CD44 receptors. |
| first_indexed | 2024-03-10T19:19:31Z |
| format | Article |
| id | doaj.art-095d57d1ce59454a8be8c18d872bd465 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T19:19:31Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-095d57d1ce59454a8be8c18d872bd4652023-11-20T03:01:51ZengMDPI AGAnimals2076-26152020-06-0110699010.3390/ani10060990Effect of Lipopolysaccharide and Muramyl Dipeptide on Apoptosis of Bovine Mammary Gland LymphocytesPetr Slama0Eliska Kabourkova1Zbysek Sladek2Terezie Zavadilova3Lucie Kratochvilova4Kristina Kharkevich5Shubhadeep Roychoudhury6Ales Pavlik7Andrea Roztocilova8Michal Uhrincat9Vladimir Tancin10Kazuhiro Kimura11Roman Konecny12Yoshio Kiku13Atsushi Watanabe14Jong-Young Kwak15Monika Zouharova16Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech RepublicDepartment of Animal Origin Food and Gastronomic Sciences, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1, 612 42 Brno, Czech RepublicDepartment of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech RepublicDepartment of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech RepublicDepartment of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech RepublicDepartment of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech RepublicDepartment of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech RepublicDepartment of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech RepublicDepartment of Animal Nutrition and Forage Production, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech RepublicNPPC-Research Institute for Animal Production, Hlohovecka 2, 951 41 Luzianky, SlovakiaNPPC-Research Institute for Animal Production, Hlohovecka 2, 951 41 Luzianky, SlovakiaLaboratory of Biochemistry, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, JapanDepartment of Animal Husbandry Sciences, Faculty of Agriculture, University of South Bohemia in Ceske Budejovice, Studentska 1668, 37005 Ceske Budejovice, Czech RepublicHokkaido Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, 4 Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-0045, JapanHokkaido Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, 4 Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-0045, JapanDepartment of Pharmacology, School of Medicine, Ajou University, Suwon 16499, KoreaDepartment of Immunology, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech RepublicThe aim of this study was to evaluate whether apoptosis of lymphocytes is modulated by stimulation by lipopolysaccharide (LPS) of <i>Escherichia coli</i> or muramyl dipeptide (MDP). Cell populations were obtained by lavaging of the mammary glands 24, 48, 72, and 168 h following intramammary induced inflammation. The portion of apoptotic lymphocytes peaked at 48 h after treatment with LPS or MDP. The analysis of CD44 expression of the same cell populations showed a higher percentage of CD44-positive lymphocytes 24- and 48-h following induction of inflammation by LPS or MDP. The results demonstrate that during both experimental infection of bovine mammary glands with LPS or MDP, apoptosis of lymphocytes was induced in the initial phase of the inflammatory response and CD44 was also overexpressed at the beginning of inflammation. These data suggest a connection of lymphocyte apoptosis with the expression of CD44 receptors.https://www.mdpi.com/2076-2615/10/6/990lipopolysaccharide<i>Escherichia coli</i>muramyl dipeptidemastitisapoptosislymphocyte |
| spellingShingle | Petr Slama Eliska Kabourkova Zbysek Sladek Terezie Zavadilova Lucie Kratochvilova Kristina Kharkevich Shubhadeep Roychoudhury Ales Pavlik Andrea Roztocilova Michal Uhrincat Vladimir Tancin Kazuhiro Kimura Roman Konecny Yoshio Kiku Atsushi Watanabe Jong-Young Kwak Monika Zouharova Effect of Lipopolysaccharide and Muramyl Dipeptide on Apoptosis of Bovine Mammary Gland Lymphocytes Animals lipopolysaccharide <i>Escherichia coli</i> muramyl dipeptide mastitis apoptosis lymphocyte |
| title | Effect of Lipopolysaccharide and Muramyl Dipeptide on Apoptosis of Bovine Mammary Gland Lymphocytes |
| title_full | Effect of Lipopolysaccharide and Muramyl Dipeptide on Apoptosis of Bovine Mammary Gland Lymphocytes |
| title_fullStr | Effect of Lipopolysaccharide and Muramyl Dipeptide on Apoptosis of Bovine Mammary Gland Lymphocytes |
| title_full_unstemmed | Effect of Lipopolysaccharide and Muramyl Dipeptide on Apoptosis of Bovine Mammary Gland Lymphocytes |
| title_short | Effect of Lipopolysaccharide and Muramyl Dipeptide on Apoptosis of Bovine Mammary Gland Lymphocytes |
| title_sort | effect of lipopolysaccharide and muramyl dipeptide on apoptosis of bovine mammary gland lymphocytes |
| topic | lipopolysaccharide <i>Escherichia coli</i> muramyl dipeptide mastitis apoptosis lymphocyte |
| url | https://www.mdpi.com/2076-2615/10/6/990 |
| work_keys_str_mv | AT petrslama effectoflipopolysaccharideandmuramyldipeptideonapoptosisofbovinemammaryglandlymphocytes AT eliskakabourkova effectoflipopolysaccharideandmuramyldipeptideonapoptosisofbovinemammaryglandlymphocytes AT zbyseksladek effectoflipopolysaccharideandmuramyldipeptideonapoptosisofbovinemammaryglandlymphocytes AT tereziezavadilova effectoflipopolysaccharideandmuramyldipeptideonapoptosisofbovinemammaryglandlymphocytes AT luciekratochvilova effectoflipopolysaccharideandmuramyldipeptideonapoptosisofbovinemammaryglandlymphocytes AT kristinakharkevich effectoflipopolysaccharideandmuramyldipeptideonapoptosisofbovinemammaryglandlymphocytes AT shubhadeeproychoudhury effectoflipopolysaccharideandmuramyldipeptideonapoptosisofbovinemammaryglandlymphocytes AT alespavlik effectoflipopolysaccharideandmuramyldipeptideonapoptosisofbovinemammaryglandlymphocytes AT andrearoztocilova effectoflipopolysaccharideandmuramyldipeptideonapoptosisofbovinemammaryglandlymphocytes AT michaluhrincat effectoflipopolysaccharideandmuramyldipeptideonapoptosisofbovinemammaryglandlymphocytes AT vladimirtancin effectoflipopolysaccharideandmuramyldipeptideonapoptosisofbovinemammaryglandlymphocytes AT kazuhirokimura effectoflipopolysaccharideandmuramyldipeptideonapoptosisofbovinemammaryglandlymphocytes AT romankonecny effectoflipopolysaccharideandmuramyldipeptideonapoptosisofbovinemammaryglandlymphocytes AT yoshiokiku effectoflipopolysaccharideandmuramyldipeptideonapoptosisofbovinemammaryglandlymphocytes AT atsushiwatanabe effectoflipopolysaccharideandmuramyldipeptideonapoptosisofbovinemammaryglandlymphocytes AT jongyoungkwak effectoflipopolysaccharideandmuramyldipeptideonapoptosisofbovinemammaryglandlymphocytes AT monikazouharova effectoflipopolysaccharideandmuramyldipeptideonapoptosisofbovinemammaryglandlymphocytes |