Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products
Approximately 1.5 trillion tons are the estimated yearly biomass production, making it an essentially unlimited source of raw material for environmentally friendly and biocompatible products transformed by microorganism, specially fungi and actinomycetes. Several lignocellulosic residues, such as si...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto de Tecnologia do Paraná (Tecpar)
2012-10-01
|
| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500020 |
| _version_ | 1828469370549960704 |
|---|---|
| author | Édilla Ribeiro dos Santos Zozilene Nascimento Santos Teles Núria Mariana Campos Diogo Angeli Jacinto de Souza Aline Simões da Rocha Bispo Rodrigo Pires do Nascimento |
| author_facet | Édilla Ribeiro dos Santos Zozilene Nascimento Santos Teles Núria Mariana Campos Diogo Angeli Jacinto de Souza Aline Simões da Rocha Bispo Rodrigo Pires do Nascimento |
| author_sort | Édilla Ribeiro dos Santos |
| collection | DOAJ |
| description | Approximately 1.5 trillion tons are the estimated yearly biomass production, making it an essentially unlimited source of raw material for environmentally friendly and biocompatible products transformed by microorganism, specially fungi and actinomycetes. Several lignocellulosic residues, such as sisal waste and sugarcane bagasse contain starch in their structures which could become important sources for the production of amylases. This study evaluated the production of amylolytic enzymes using Streptomyces sp. SLBA-08 strain, isolated from a semi-arid soil, according to their ability to grow on soluble starch as the sole carbon source. The effect of the carbon source (sisal waste and sugarcane bagasse) on α-amylase production was studied using submerged cultivations at 30 ºC. The highest level of α-amylase activity corresponded to 10.1 U. mL-1 and was obtained using sisal waste (2.7%) and urea (0.8%) in submerged fermentation after 3 days of cultivation. The partial characterization showed the best α-amylase activity at 50ºC and pH 7.0. These results are of great importance for the use of sisal waste as a substrate for biotechnological proposes. |
| first_indexed | 2024-12-11T04:38:32Z |
| format | Article |
| id | doaj.art-095fcb031cdb473d95691ccbde8a2de4 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-11T04:38:32Z |
| publishDate | 2012-10-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-095fcb031cdb473d95691ccbde8a2de42022-12-22T01:20:41ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242012-10-0155579380010.1590/S1516-89132012000500020Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-productsÉdilla Ribeiro dos SantosZozilene Nascimento Santos TelesNúria Mariana CamposDiogo Angeli Jacinto de SouzaAline Simões da Rocha BispoRodrigo Pires do NascimentoApproximately 1.5 trillion tons are the estimated yearly biomass production, making it an essentially unlimited source of raw material for environmentally friendly and biocompatible products transformed by microorganism, specially fungi and actinomycetes. Several lignocellulosic residues, such as sisal waste and sugarcane bagasse contain starch in their structures which could become important sources for the production of amylases. This study evaluated the production of amylolytic enzymes using Streptomyces sp. SLBA-08 strain, isolated from a semi-arid soil, according to their ability to grow on soluble starch as the sole carbon source. The effect of the carbon source (sisal waste and sugarcane bagasse) on α-amylase production was studied using submerged cultivations at 30 ºC. The highest level of α-amylase activity corresponded to 10.1 U. mL-1 and was obtained using sisal waste (2.7%) and urea (0.8%) in submerged fermentation after 3 days of cultivation. The partial characterization showed the best α-amylase activity at 50ºC and pH 7.0. These results are of great importance for the use of sisal waste as a substrate for biotechnological proposes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500020α-amylasessugarcane bagassesisal wasteStreptomyces sp |
| spellingShingle | Édilla Ribeiro dos Santos Zozilene Nascimento Santos Teles Núria Mariana Campos Diogo Angeli Jacinto de Souza Aline Simões da Rocha Bispo Rodrigo Pires do Nascimento Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products Brazilian Archives of Biology and Technology α-amylases sugarcane bagasse sisal waste Streptomyces sp |
| title | Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products |
| title_full | Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products |
| title_fullStr | Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products |
| title_full_unstemmed | Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products |
| title_short | Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products |
| title_sort | production of 945 amylase from streptomyces sp slba 08 strain using agro industrial by products |
| topic | α-amylases sugarcane bagasse sisal waste Streptomyces sp |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500020 |
| work_keys_str_mv | AT edillaribeirodossantos productionof945amylasefromstreptomycesspslba08strainusingagroindustrialbyproducts AT zozilenenascimentosantosteles productionof945amylasefromstreptomycesspslba08strainusingagroindustrialbyproducts AT nuriamarianacampos productionof945amylasefromstreptomycesspslba08strainusingagroindustrialbyproducts AT diogoangelijacintodesouza productionof945amylasefromstreptomycesspslba08strainusingagroindustrialbyproducts AT alinesimoesdarochabispo productionof945amylasefromstreptomycesspslba08strainusingagroindustrialbyproducts AT rodrigopiresdonascimento productionof945amylasefromstreptomycesspslba08strainusingagroindustrialbyproducts |