Effects of dicamba and casein hydrolysate on in vitro growthand shoot regeneration of date palm (Phoenix dactylifera L.) cv. Barhee
The investigation was carried out to evaluate the influence of the dicamba (3,6-Dichloro-2-methoxybenzoic acid) (DIC) and casein hydrolysate (CH) on the callus growth, shoot multiplication, and some biochemical constituents of date palm cv. Barhee cultured in vitro. Both DIC and CH were required for...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2024-01-01
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| Series: | Folia Oecologica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/foecol-2024-0006 |
| _version_ | 1797340391568048128 |
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| author | Al-Asadi Ahmed Z.R. Al-Mayahi Ahmed M.W. Awad Khairullah M. |
| author_facet | Al-Asadi Ahmed Z.R. Al-Mayahi Ahmed M.W. Awad Khairullah M. |
| author_sort | Al-Asadi Ahmed Z.R. |
| collection | DOAJ |
| description | The investigation was carried out to evaluate the influence of the dicamba (3,6-Dichloro-2-methoxybenzoic acid) (DIC) and casein hydrolysate (CH) on the callus growth, shoot multiplication, and some biochemical constituents of date palm cv. Barhee cultured in vitro. Both DIC and CH were required for callus growth and shoots regeneration. The medium supplemented with 4.0 mg l−1 DIC in combination with 1.0 g l−1 CH gave the highest callus weight (287 mg), while the maximum response rate and the number of shoots per jar (86.67% and 15.07 shoots/jar) were found in MS media equipped with 4 mg l−1 DIC and 0.5 mg l−1 CH combination. The total amount of phenolic compounds was significantly reduced to 0.82 and 0.79 mg GAE g–1 in shoots cultured in the medium equipped with 4.0 mg l−1 DIC with 0.5 and 1.0 g l−1 CH, which is reflected in the rate of browning. The results showed that the highest shoots content of endogenous IAA (3.71 and 3.50 μg g−1), were obtained in response to 4 mg l−1 DIC + 1.0 g l−1 CH and 4.0 mg l−1 DIC + 0.5 g l−1 CH, respectively. The macronutrient K, P, Ca, and free amino acids content significantly increased in the in vitro shoots regenerated on the media supplemented with 4.0 mg l−1 DIC + 1.0 g l−1 CH. The genetic stability of this study was confirmed by the DNA-based fingerprinting method RAPD. The RAPD binding patterns indicated no variation among tissue culture-derived plants. The in vitro propagation protocol described herein can be introduced to the production of genetically stable date palm plants. |
| first_indexed | 2024-03-08T10:02:25Z |
| format | Article |
| id | doaj.art-09620851800547178b6027069e6f0284 |
| institution | Directory Open Access Journal |
| issn | 1338-7014 |
| language | English |
| last_indexed | 2024-03-08T10:02:25Z |
| publishDate | 2024-01-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Folia Oecologica |
| spelling | doaj.art-09620851800547178b6027069e6f02842024-01-29T08:53:29ZengSciendoFolia Oecologica1338-70142024-01-01511566510.2478/foecol-2024-0006Effects of dicamba and casein hydrolysate on in vitro growthand shoot regeneration of date palm (Phoenix dactylifera L.) cv. BarheeAl-Asadi Ahmed Z.R.0Al-Mayahi Ahmed M.W.1Awad Khairullah M.21Horticulture and Landscape Department, Agriculture College, Basrah University, Basrah, IraqDate Palm Research Centre, Basrah University, Basrah, IraqDate Palm Research Centre, Basrah University, Basrah, IraqThe investigation was carried out to evaluate the influence of the dicamba (3,6-Dichloro-2-methoxybenzoic acid) (DIC) and casein hydrolysate (CH) on the callus growth, shoot multiplication, and some biochemical constituents of date palm cv. Barhee cultured in vitro. Both DIC and CH were required for callus growth and shoots regeneration. The medium supplemented with 4.0 mg l−1 DIC in combination with 1.0 g l−1 CH gave the highest callus weight (287 mg), while the maximum response rate and the number of shoots per jar (86.67% and 15.07 shoots/jar) were found in MS media equipped with 4 mg l−1 DIC and 0.5 mg l−1 CH combination. The total amount of phenolic compounds was significantly reduced to 0.82 and 0.79 mg GAE g–1 in shoots cultured in the medium equipped with 4.0 mg l−1 DIC with 0.5 and 1.0 g l−1 CH, which is reflected in the rate of browning. The results showed that the highest shoots content of endogenous IAA (3.71 and 3.50 μg g−1), were obtained in response to 4 mg l−1 DIC + 1.0 g l−1 CH and 4.0 mg l−1 DIC + 0.5 g l−1 CH, respectively. The macronutrient K, P, Ca, and free amino acids content significantly increased in the in vitro shoots regenerated on the media supplemented with 4.0 mg l−1 DIC + 1.0 g l−1 CH. The genetic stability of this study was confirmed by the DNA-based fingerprinting method RAPD. The RAPD binding patterns indicated no variation among tissue culture-derived plants. The in vitro propagation protocol described herein can be introduced to the production of genetically stable date palm plants.https://doi.org/10.2478/foecol-2024-0006amino acidsauxinin vitromacronutrientrapdshoot regeneration |
| spellingShingle | Al-Asadi Ahmed Z.R. Al-Mayahi Ahmed M.W. Awad Khairullah M. Effects of dicamba and casein hydrolysate on in vitro growthand shoot regeneration of date palm (Phoenix dactylifera L.) cv. Barhee Folia Oecologica amino acids auxin in vitro macronutrient rapd shoot regeneration |
| title | Effects of dicamba and casein hydrolysate on in vitro growthand shoot regeneration of date palm (Phoenix dactylifera L.) cv. Barhee |
| title_full | Effects of dicamba and casein hydrolysate on in vitro growthand shoot regeneration of date palm (Phoenix dactylifera L.) cv. Barhee |
| title_fullStr | Effects of dicamba and casein hydrolysate on in vitro growthand shoot regeneration of date palm (Phoenix dactylifera L.) cv. Barhee |
| title_full_unstemmed | Effects of dicamba and casein hydrolysate on in vitro growthand shoot regeneration of date palm (Phoenix dactylifera L.) cv. Barhee |
| title_short | Effects of dicamba and casein hydrolysate on in vitro growthand shoot regeneration of date palm (Phoenix dactylifera L.) cv. Barhee |
| title_sort | effects of dicamba and casein hydrolysate on in vitro growthand shoot regeneration of date palm phoenix dactylifera l cv barhee |
| topic | amino acids auxin in vitro macronutrient rapd shoot regeneration |
| url | https://doi.org/10.2478/foecol-2024-0006 |
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