Comparative transcriptional analysis of <it>Bacillus subtilis</it> cells overproducing either secreted proteins, lipoproteins or membrane proteins
<p>Abstract</p> <p>Background</p> <p><it>Bacillus subtilis</it> is a favorable host for the production of industrially relevant proteins because of its capacity of secreting proteins into the medium to high levels, its GRAS (Generally Recognized As Safe) sta...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-05-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | http://www.microbialcellfactories.com/content/11/1/66 |
| _version_ | 1811287354250362880 |
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| author | Marciniak Bogumiła C Trip Hein van-der Veek Patricia J Kuipers Oscar P |
| author_facet | Marciniak Bogumiła C Trip Hein van-der Veek Patricia J Kuipers Oscar P |
| author_sort | Marciniak Bogumiła C |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Bacillus subtilis</it> is a favorable host for the production of industrially relevant proteins because of its capacity of secreting proteins into the medium to high levels, its GRAS (Generally Recognized As Safe) status, its genetic accessibility and its capacity to grow in large fermentations. However, production of heterologous proteins still faces limitations.</p> <p>Results</p> <p>This study aimed at the identification of bottlenecks in secretory protein production by analyzing the response of <it>B. subtilis</it> at the transcriptome level to overproduction of eight secretory proteins of endogenous and heterologous origin and with different subcellular or extracellular destination: secreted proteins (NprE and XynA of <it>B. subtilis</it>, Usp45 of <it>Lactococcus lactis</it>, TEM-1 β-lactamase of <it>Escherichia coli</it>), membrane proteins (LmrA of <it>L. lactis</it> and XylP of <it>Lactobacillus pentosus</it>) and lipoproteins (MntA and YcdH of <it>B. subtilis</it>). Responses specific for proteins with a common localization as well as more general stress responses were observed. The latter include upregulation of genes encoding intracellular stress proteins (<it>groES</it>/<it>EL</it>, CtsR regulated genes). Specific responses include upregulation of the <it>liaIHGFSR</it> operon under Usp45 and TEM-1 β-lactamase overproduction; <it>cssRS</it>, <it>htrA</it> and <it>htrB</it> under all secreted proteins overproduction; <it>sigW</it> and SigW-regulated genes mainly under membrane proteins overproduction; and <it>ykrL</it> (encoding an HtpX homologue) specifically under membrane proteins overproduction.</p> <p>Conclusions</p> <p>The results give better insights into <it>B. subtilis</it> responses to protein overproduction stress and provide potential targets for genetic engineering in order to further improve <it>B. subtilis</it> as a protein production host.</p> |
| first_indexed | 2024-04-13T03:17:05Z |
| format | Article |
| id | doaj.art-096381f92b024761a6316f30751942bf |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-13T03:17:05Z |
| publishDate | 2012-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-096381f92b024761a6316f30751942bf2022-12-22T03:04:52ZengBMCMicrobial Cell Factories1475-28592012-05-011116610.1186/1475-2859-11-66Comparative transcriptional analysis of <it>Bacillus subtilis</it> cells overproducing either secreted proteins, lipoproteins or membrane proteinsMarciniak Bogumiła CTrip Heinvan-der Veek Patricia JKuipers Oscar P<p>Abstract</p> <p>Background</p> <p><it>Bacillus subtilis</it> is a favorable host for the production of industrially relevant proteins because of its capacity of secreting proteins into the medium to high levels, its GRAS (Generally Recognized As Safe) status, its genetic accessibility and its capacity to grow in large fermentations. However, production of heterologous proteins still faces limitations.</p> <p>Results</p> <p>This study aimed at the identification of bottlenecks in secretory protein production by analyzing the response of <it>B. subtilis</it> at the transcriptome level to overproduction of eight secretory proteins of endogenous and heterologous origin and with different subcellular or extracellular destination: secreted proteins (NprE and XynA of <it>B. subtilis</it>, Usp45 of <it>Lactococcus lactis</it>, TEM-1 β-lactamase of <it>Escherichia coli</it>), membrane proteins (LmrA of <it>L. lactis</it> and XylP of <it>Lactobacillus pentosus</it>) and lipoproteins (MntA and YcdH of <it>B. subtilis</it>). Responses specific for proteins with a common localization as well as more general stress responses were observed. The latter include upregulation of genes encoding intracellular stress proteins (<it>groES</it>/<it>EL</it>, CtsR regulated genes). Specific responses include upregulation of the <it>liaIHGFSR</it> operon under Usp45 and TEM-1 β-lactamase overproduction; <it>cssRS</it>, <it>htrA</it> and <it>htrB</it> under all secreted proteins overproduction; <it>sigW</it> and SigW-regulated genes mainly under membrane proteins overproduction; and <it>ykrL</it> (encoding an HtpX homologue) specifically under membrane proteins overproduction.</p> <p>Conclusions</p> <p>The results give better insights into <it>B. subtilis</it> responses to protein overproduction stress and provide potential targets for genetic engineering in order to further improve <it>B. subtilis</it> as a protein production host.</p>http://www.microbialcellfactories.com/content/11/1/66Protein overproductionSecretion stressProduction host<it>sigW</it><it>ykrL</it>LiaRSMembraneCell wall |
| spellingShingle | Marciniak Bogumiła C Trip Hein van-der Veek Patricia J Kuipers Oscar P Comparative transcriptional analysis of <it>Bacillus subtilis</it> cells overproducing either secreted proteins, lipoproteins or membrane proteins Microbial Cell Factories Protein overproduction Secretion stress Production host <it>sigW</it> <it>ykrL</it> LiaRS Membrane Cell wall |
| title | Comparative transcriptional analysis of <it>Bacillus subtilis</it> cells overproducing either secreted proteins, lipoproteins or membrane proteins |
| title_full | Comparative transcriptional analysis of <it>Bacillus subtilis</it> cells overproducing either secreted proteins, lipoproteins or membrane proteins |
| title_fullStr | Comparative transcriptional analysis of <it>Bacillus subtilis</it> cells overproducing either secreted proteins, lipoproteins or membrane proteins |
| title_full_unstemmed | Comparative transcriptional analysis of <it>Bacillus subtilis</it> cells overproducing either secreted proteins, lipoproteins or membrane proteins |
| title_short | Comparative transcriptional analysis of <it>Bacillus subtilis</it> cells overproducing either secreted proteins, lipoproteins or membrane proteins |
| title_sort | comparative transcriptional analysis of it bacillus subtilis it cells overproducing either secreted proteins lipoproteins or membrane proteins |
| topic | Protein overproduction Secretion stress Production host <it>sigW</it> <it>ykrL</it> LiaRS Membrane Cell wall |
| url | http://www.microbialcellfactories.com/content/11/1/66 |
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