The Relationship Between Transcript Expression Levels of Nuclear Encoded (TFAM, NRF1) and Mitochondrial Encoded (MT-CO1) Genes in Single Human Oocytes During Oocyte Maturation
In some cases of infertility in women, human oocytes fail to mature when they reach the metaphase II (MII) stage. Mitochondria plays an important role in oocyte maturation. A large number of mitochondrial DNA (mtDNA), copied in oocytes, is essential for providing adenosine triphosphate (ATP) during...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2015-06-01
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| Series: | Balkan Journal of Medical Genetics |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/bjmg-2015-0004 |
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| author | Ghaffari Novin M. Allahveisi Azra Noruzinia M. Farhadifar F. Yousefian E. Dehghani Fard A. Salimi M. |
| author_facet | Ghaffari Novin M. Allahveisi Azra Noruzinia M. Farhadifar F. Yousefian E. Dehghani Fard A. Salimi M. |
| author_sort | Ghaffari Novin M. |
| collection | DOAJ |
| description | In some cases of infertility in women, human oocytes fail to mature when they reach the metaphase II (MII) stage. Mitochondria plays an important role in oocyte maturation. A large number of mitochondrial DNA (mtDNA), copied in oocytes, is essential for providing adenosine triphosphate (ATP) during oocyte maturation. The purpose of this study was to identify the relationship between transcript expression levels of the mitochondrial encoded gene (MT-CO1) and two nuclear encoded genes, nuclear respiratory factor 1 (NRF1) and mitochondrial transcription factor A (TFAM) in various stages of human oocyte maturation. Nine consenting patients, age 21-35 years old, with male factors were selected for ovarian stimulation and intracytoplasmic sperm injection (ICSI) procedures. mRNA levels of mitochondrial- related genes were performed by singlecell TaqMan® quantitative real-time polymerase chain reaction (qRT-PCR). There was no significant relationship between the relative expression levels in germinal vesicle (GV) stage oocytes (p = 0.62). On the contrary, a significant relationship was seen between the relative expression levels of TFAM and NRF1 and the MT-CO1 genes at the stages of metaphase I (MI) and MII (p = 0.03 and p = 0.002). A relationship exists between the transcript expression levels of TFAM and NRF1, and MT-CO1 genes in various stages of human oocyte maturation. |
| first_indexed | 2024-03-12T19:04:51Z |
| format | Article |
| id | doaj.art-096415332ded4367a1d4a7be11a9efa2 |
| institution | Directory Open Access Journal |
| issn | 1311-0160 |
| language | English |
| last_indexed | 2024-03-12T19:04:51Z |
| publishDate | 2015-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Balkan Journal of Medical Genetics |
| spelling | doaj.art-096415332ded4367a1d4a7be11a9efa22023-08-02T06:19:02ZengSciendoBalkan Journal of Medical Genetics1311-01602015-06-01181394610.1515/bjmg-2015-0004bjmg-2015-0004The Relationship Between Transcript Expression Levels of Nuclear Encoded (TFAM, NRF1) and Mitochondrial Encoded (MT-CO1) Genes in Single Human Oocytes During Oocyte MaturationGhaffari Novin M.0Allahveisi Azra1Noruzinia M.2Farhadifar F.3Yousefian E.4Dehghani Fard A.5Salimi M.6Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Science, Tehran, IranDepartment of Anatomical Sciences, Faculty of Medicine, Kurdistan University of Medical Sciences, Pasdaran Street, Sanandaj, Iran. Tel: +98-873-664-673. Fax: +98-873-364-674Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDeptartment of Obstetrics and Gynecology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IranDepartment of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranSarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, IranDepartment of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Science, Tehran, IranIn some cases of infertility in women, human oocytes fail to mature when they reach the metaphase II (MII) stage. Mitochondria plays an important role in oocyte maturation. A large number of mitochondrial DNA (mtDNA), copied in oocytes, is essential for providing adenosine triphosphate (ATP) during oocyte maturation. The purpose of this study was to identify the relationship between transcript expression levels of the mitochondrial encoded gene (MT-CO1) and two nuclear encoded genes, nuclear respiratory factor 1 (NRF1) and mitochondrial transcription factor A (TFAM) in various stages of human oocyte maturation. Nine consenting patients, age 21-35 years old, with male factors were selected for ovarian stimulation and intracytoplasmic sperm injection (ICSI) procedures. mRNA levels of mitochondrial- related genes were performed by singlecell TaqMan® quantitative real-time polymerase chain reaction (qRT-PCR). There was no significant relationship between the relative expression levels in germinal vesicle (GV) stage oocytes (p = 0.62). On the contrary, a significant relationship was seen between the relative expression levels of TFAM and NRF1 and the MT-CO1 genes at the stages of metaphase I (MI) and MII (p = 0.03 and p = 0.002). A relationship exists between the transcript expression levels of TFAM and NRF1, and MT-CO1 genes in various stages of human oocyte maturation.https://doi.org/10.1515/bjmg-2015-0004mitochondriaoocyte maturationquantitative real-time polymerase chain reaction (qrt-pcr)single-cell |
| spellingShingle | Ghaffari Novin M. Allahveisi Azra Noruzinia M. Farhadifar F. Yousefian E. Dehghani Fard A. Salimi M. The Relationship Between Transcript Expression Levels of Nuclear Encoded (TFAM, NRF1) and Mitochondrial Encoded (MT-CO1) Genes in Single Human Oocytes During Oocyte Maturation Balkan Journal of Medical Genetics mitochondria oocyte maturation quantitative real-time polymerase chain reaction (qrt-pcr) single-cell |
| title | The Relationship Between Transcript Expression Levels of Nuclear Encoded (TFAM, NRF1) and Mitochondrial Encoded (MT-CO1) Genes in Single Human Oocytes During Oocyte Maturation |
| title_full | The Relationship Between Transcript Expression Levels of Nuclear Encoded (TFAM, NRF1) and Mitochondrial Encoded (MT-CO1) Genes in Single Human Oocytes During Oocyte Maturation |
| title_fullStr | The Relationship Between Transcript Expression Levels of Nuclear Encoded (TFAM, NRF1) and Mitochondrial Encoded (MT-CO1) Genes in Single Human Oocytes During Oocyte Maturation |
| title_full_unstemmed | The Relationship Between Transcript Expression Levels of Nuclear Encoded (TFAM, NRF1) and Mitochondrial Encoded (MT-CO1) Genes in Single Human Oocytes During Oocyte Maturation |
| title_short | The Relationship Between Transcript Expression Levels of Nuclear Encoded (TFAM, NRF1) and Mitochondrial Encoded (MT-CO1) Genes in Single Human Oocytes During Oocyte Maturation |
| title_sort | relationship between transcript expression levels of nuclear encoded tfam nrf1 and mitochondrial encoded mt co1 genes in single human oocytes during oocyte maturation |
| topic | mitochondria oocyte maturation quantitative real-time polymerase chain reaction (qrt-pcr) single-cell |
| url | https://doi.org/10.1515/bjmg-2015-0004 |
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