Genotoxicity assessment of saline extract from Pilosocereus gounellei (Cactaceae) and its chemopreventive effect against cyclophosphamide-induced DNA damage
Pilosocereus gounellei (Cactaceae) is used to treat wounds and inflammation. In this study, we evaluated whether the saline extract from its stem would have genotoxic or anti-genotoxic effects. In the genotoxicity evaluation, mice received the extract (500, 1,000, or 2,000 mg/kg) orally while negati...
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Elsevier
2020-04-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020306563 |
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| author | Alisson Macário de Oliveira Anderson Felipe Soares de Freitas Patrícia Maria Guedes Paiva Thiago Henrique Napoleão |
| author_facet | Alisson Macário de Oliveira Anderson Felipe Soares de Freitas Patrícia Maria Guedes Paiva Thiago Henrique Napoleão |
| author_sort | Alisson Macário de Oliveira |
| collection | DOAJ |
| description | Pilosocereus gounellei (Cactaceae) is used to treat wounds and inflammation. In this study, we evaluated whether the saline extract from its stem would have genotoxic or anti-genotoxic effects. In the genotoxicity evaluation, mice received the extract (500, 1,000, or 2,000 mg/kg) orally while negative and positive controls were treated with saline solution (0.9% NaCl) per os and cyclophosphamide (CPA, 80 mg/kg i.p.), respectively. In the anti-genotoxicity assay, using other animals, treatments were carried out by administering the extract (500, 1,000 or 2,000 mg/kg) or saline solution (negative control) per os and then CPA (80 mg/kg i.p.) 1 h later. Genotoxic effects were evaluated by micronucleus test and comet assay using peripheral blood and bone marrow cells. Oral administration of only the extract at 500 and 1,000 mg/kg did not result in genotoxicity. A slight increase in the incidence of micronucleus was observed at the highest dose (2,000 mg/kg). Administration of the extract before CPA reduced the micronucleated polychromatic erythrocytes (MNPCE) number by 49.07–71.43%, and DNA fragmentation in peripheral blood (85.04–94.44%) and bone marrow (87.43–92.70%) cells also decreased. In conclusion, when administered orally at the tested doses, the extract is genotoxically safe, being cautious in doses above 1,000 mg/kg, and has a protective effect against CPA-induced DNA damage. |
| first_indexed | 2024-12-10T04:14:46Z |
| format | Article |
| id | doaj.art-09642192ff9e4bb98395cfd76e3e0b23 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-10T04:14:46Z |
| publishDate | 2020-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-09642192ff9e4bb98395cfd76e3e0b232022-12-22T02:02:36ZengElsevierHeliyon2405-84402020-04-0164e03811Genotoxicity assessment of saline extract from Pilosocereus gounellei (Cactaceae) and its chemopreventive effect against cyclophosphamide-induced DNA damageAlisson Macário de Oliveira0Anderson Felipe Soares de Freitas1Patrícia Maria Guedes Paiva2Thiago Henrique Napoleão3Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, BrazilDepartamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, BrazilDepartamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, BrazilCorresponding author.; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, BrazilPilosocereus gounellei (Cactaceae) is used to treat wounds and inflammation. In this study, we evaluated whether the saline extract from its stem would have genotoxic or anti-genotoxic effects. In the genotoxicity evaluation, mice received the extract (500, 1,000, or 2,000 mg/kg) orally while negative and positive controls were treated with saline solution (0.9% NaCl) per os and cyclophosphamide (CPA, 80 mg/kg i.p.), respectively. In the anti-genotoxicity assay, using other animals, treatments were carried out by administering the extract (500, 1,000 or 2,000 mg/kg) or saline solution (negative control) per os and then CPA (80 mg/kg i.p.) 1 h later. Genotoxic effects were evaluated by micronucleus test and comet assay using peripheral blood and bone marrow cells. Oral administration of only the extract at 500 and 1,000 mg/kg did not result in genotoxicity. A slight increase in the incidence of micronucleus was observed at the highest dose (2,000 mg/kg). Administration of the extract before CPA reduced the micronucleated polychromatic erythrocytes (MNPCE) number by 49.07–71.43%, and DNA fragmentation in peripheral blood (85.04–94.44%) and bone marrow (87.43–92.70%) cells also decreased. In conclusion, when administered orally at the tested doses, the extract is genotoxically safe, being cautious in doses above 1,000 mg/kg, and has a protective effect against CPA-induced DNA damage.http://www.sciencedirect.com/science/article/pii/S2405844020306563CactusMicronucleiComet assayAnti-Genotoxic effectBiological sciencesMetabolite |
| spellingShingle | Alisson Macário de Oliveira Anderson Felipe Soares de Freitas Patrícia Maria Guedes Paiva Thiago Henrique Napoleão Genotoxicity assessment of saline extract from Pilosocereus gounellei (Cactaceae) and its chemopreventive effect against cyclophosphamide-induced DNA damage Heliyon Cactus Micronuclei Comet assay Anti-Genotoxic effect Biological sciences Metabolite |
| title | Genotoxicity assessment of saline extract from Pilosocereus gounellei (Cactaceae) and its chemopreventive effect against cyclophosphamide-induced DNA damage |
| title_full | Genotoxicity assessment of saline extract from Pilosocereus gounellei (Cactaceae) and its chemopreventive effect against cyclophosphamide-induced DNA damage |
| title_fullStr | Genotoxicity assessment of saline extract from Pilosocereus gounellei (Cactaceae) and its chemopreventive effect against cyclophosphamide-induced DNA damage |
| title_full_unstemmed | Genotoxicity assessment of saline extract from Pilosocereus gounellei (Cactaceae) and its chemopreventive effect against cyclophosphamide-induced DNA damage |
| title_short | Genotoxicity assessment of saline extract from Pilosocereus gounellei (Cactaceae) and its chemopreventive effect against cyclophosphamide-induced DNA damage |
| title_sort | genotoxicity assessment of saline extract from pilosocereus gounellei cactaceae and its chemopreventive effect against cyclophosphamide induced dna damage |
| topic | Cactus Micronuclei Comet assay Anti-Genotoxic effect Biological sciences Metabolite |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020306563 |
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