Effects of Biotin on survival, ensheathment, and ATP production by oligodendrocyte lineage cells in vitro.
Mechanisms implicated in disease progression in multiple sclerosis include continued oligodendrocyte (OL)/myelin injury and failure of myelin repair. Underlying causes include metabolic stress with resultant energy deficiency. Biotin is a cofactor for carboxylases involved in ATP production that imp...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0233859 |
| _version_ | 1819041892694753280 |
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| author | Qiao-Ling Cui Yun Hsuan Lin Yu Kang T Xu Milton G F Fernandes Vijayaraghava T S Rao Timothy E Kennedy Jack Antel |
| author_facet | Qiao-Ling Cui Yun Hsuan Lin Yu Kang T Xu Milton G F Fernandes Vijayaraghava T S Rao Timothy E Kennedy Jack Antel |
| author_sort | Qiao-Ling Cui |
| collection | DOAJ |
| description | Mechanisms implicated in disease progression in multiple sclerosis include continued oligodendrocyte (OL)/myelin injury and failure of myelin repair. Underlying causes include metabolic stress with resultant energy deficiency. Biotin is a cofactor for carboxylases involved in ATP production that impact myelin production by promoting fatty acid synthesis. Here, we investigate the effects of high dose Biotin (MD1003) on the functional properties of post-natal rat derived oligodendrocyte progenitor cells (OPCs). A2B5 positive OPCs were assessed using an in vitro injury assay, culturing cells in either DFM (DMEM/F12+N1) or "stress media" (no glucose (NG)-DMEM), with Biotin added over a range from 2.5 to 250 μg/ml, and cell viability determined after 24 hrs. Biotin reduced the increase in OPC cell death in the NG condition. In nanofiber myelination assays, biotin increased the percentage of ensheathing cells, the number of ensheathed segments per cell, and length of ensheathed segments. In dispersed cell culture, Biotin also significantly increased ATP production, assessed using a Seahorse bio-analyzer. For most assays, the positive effects of Biotin were observed at the higher end of the dose-response analysis. We conclude that Biotin, in vitro, protects OL lineage cells from metabolic injury, enhances myelin-like ensheathment, and is associated with increased ATP production. |
| first_indexed | 2024-12-21T09:32:13Z |
| format | Article |
| id | doaj.art-09686c74f7a94ab3980342f065c58fe5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T09:32:13Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-09686c74f7a94ab3980342f065c58fe52022-12-21T19:08:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01155e023385910.1371/journal.pone.0233859Effects of Biotin on survival, ensheathment, and ATP production by oligodendrocyte lineage cells in vitro.Qiao-Ling CuiYun Hsuan LinYu Kang T XuMilton G F FernandesVijayaraghava T S RaoTimothy E KennedyJack AntelMechanisms implicated in disease progression in multiple sclerosis include continued oligodendrocyte (OL)/myelin injury and failure of myelin repair. Underlying causes include metabolic stress with resultant energy deficiency. Biotin is a cofactor for carboxylases involved in ATP production that impact myelin production by promoting fatty acid synthesis. Here, we investigate the effects of high dose Biotin (MD1003) on the functional properties of post-natal rat derived oligodendrocyte progenitor cells (OPCs). A2B5 positive OPCs were assessed using an in vitro injury assay, culturing cells in either DFM (DMEM/F12+N1) or "stress media" (no glucose (NG)-DMEM), with Biotin added over a range from 2.5 to 250 μg/ml, and cell viability determined after 24 hrs. Biotin reduced the increase in OPC cell death in the NG condition. In nanofiber myelination assays, biotin increased the percentage of ensheathing cells, the number of ensheathed segments per cell, and length of ensheathed segments. In dispersed cell culture, Biotin also significantly increased ATP production, assessed using a Seahorse bio-analyzer. For most assays, the positive effects of Biotin were observed at the higher end of the dose-response analysis. We conclude that Biotin, in vitro, protects OL lineage cells from metabolic injury, enhances myelin-like ensheathment, and is associated with increased ATP production.https://doi.org/10.1371/journal.pone.0233859 |
| spellingShingle | Qiao-Ling Cui Yun Hsuan Lin Yu Kang T Xu Milton G F Fernandes Vijayaraghava T S Rao Timothy E Kennedy Jack Antel Effects of Biotin on survival, ensheathment, and ATP production by oligodendrocyte lineage cells in vitro. PLoS ONE |
| title | Effects of Biotin on survival, ensheathment, and ATP production by oligodendrocyte lineage cells in vitro. |
| title_full | Effects of Biotin on survival, ensheathment, and ATP production by oligodendrocyte lineage cells in vitro. |
| title_fullStr | Effects of Biotin on survival, ensheathment, and ATP production by oligodendrocyte lineage cells in vitro. |
| title_full_unstemmed | Effects of Biotin on survival, ensheathment, and ATP production by oligodendrocyte lineage cells in vitro. |
| title_short | Effects of Biotin on survival, ensheathment, and ATP production by oligodendrocyte lineage cells in vitro. |
| title_sort | effects of biotin on survival ensheathment and atp production by oligodendrocyte lineage cells in vitro |
| url | https://doi.org/10.1371/journal.pone.0233859 |
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