Gold Half-Shell-Coated Paclitaxel-Loaded PLGA Nanoparticles for the Targeted Chemo-Photothermal Treatment of Cancer
Conventional cancer therapies suffer from nonspecificity, drug resistance, and a poor bioavailability, which trigger severe side effects. To overcome these disadvantages, in this study, we designed and evaluated the in vitro potential of paclitaxel-loaded, PLGA-gold, half-shell nanoparticles (PTX-PL...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/14/7/1390 |
| _version_ | 1797588135428751360 |
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| author | Jaime Ibarra David Encinas-Basurto Mario Almada Josué Juárez Miguel Angel Valdez Silvia Barbosa Pablo Taboada |
| author_facet | Jaime Ibarra David Encinas-Basurto Mario Almada Josué Juárez Miguel Angel Valdez Silvia Barbosa Pablo Taboada |
| author_sort | Jaime Ibarra |
| collection | DOAJ |
| description | Conventional cancer therapies suffer from nonspecificity, drug resistance, and a poor bioavailability, which trigger severe side effects. To overcome these disadvantages, in this study, we designed and evaluated the in vitro potential of paclitaxel-loaded, PLGA-gold, half-shell nanoparticles (PTX-PLGA/Au-HS NPs) conjugated with cyclo(Arg-Gly-Asp-Phe-Lys) (cyRGDfk) as a targeted chemo-photothermal therapy system in HeLa and MDA-MB-231 cancer cells. A TEM analysis confirmed the successful gold half-shell structure formation. High-performance liquid chromatography showed an encapsulation efficiency of the paclitaxel inside nanoparticles of more than 90%. In the release study, an initial burst release of about 20% in the first 24 h was observed, followed by a sustained drug release for a period as long as 10 days, reaching values of about 92% and 49% for NPs with and without near infrared laser irradiation. In in vitro cell internalization studies, targeted nanoparticles showed a higher accumulation than nontargeted nanoparticles, possibly through a specific interaction of the cyRGDfk with their homologous receptors, the ανβ3 y ανβ5 integrins on the cell surface. Compared with chemotherapy or photothermal treatment alone, the combined treatment demonstrated a synergistic effect, reducing the cell viability to 23% for the HeLa cells and 31% for the MDA-MB-231 cells. Thus, our results indicate that these multifuncional nanoparticles can be considered to be a promising targeted chemo-photothermal therapy system against cancer. |
| first_indexed | 2024-03-11T00:48:30Z |
| format | Article |
| id | doaj.art-0968e2650f2549cca60f8b5b123670c8 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-11T00:48:30Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-0968e2650f2549cca60f8b5b123670c82023-11-18T20:32:45ZengMDPI AGMicromachines2072-666X2023-07-01147139010.3390/mi14071390Gold Half-Shell-Coated Paclitaxel-Loaded PLGA Nanoparticles for the Targeted Chemo-Photothermal Treatment of CancerJaime Ibarra0David Encinas-Basurto1Mario Almada2Josué Juárez3Miguel Angel Valdez4Silvia Barbosa5Pablo Taboada6Departamento de Física, Matemáticas e Ingeniería, Universidad de Sonora, Campus Navojoa, Navojoa 85880, Sonora, MexicoDepartamento de Física, Matemáticas e Ingeniería, Universidad de Sonora, Campus Navojoa, Navojoa 85880, Sonora, MexicoDepartamento de Ciencias Químico-Biológicas y Agropecuarias, Universidad de Sonora, Campus Navojoa, Navojoa 85880, Sonora, MexicoDepartamento de Física, Universidad de Sonora, Campus Hermosillo, Hermosillo 83000, Sonora, MexicoDepartamento de Física, Universidad de Sonora, Campus Hermosillo, Hermosillo 83000, Sonora, MexicoDepartamento de Física de Partículas, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, A Coruña, SpainDepartamento de Física de Partículas, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, A Coruña, SpainConventional cancer therapies suffer from nonspecificity, drug resistance, and a poor bioavailability, which trigger severe side effects. To overcome these disadvantages, in this study, we designed and evaluated the in vitro potential of paclitaxel-loaded, PLGA-gold, half-shell nanoparticles (PTX-PLGA/Au-HS NPs) conjugated with cyclo(Arg-Gly-Asp-Phe-Lys) (cyRGDfk) as a targeted chemo-photothermal therapy system in HeLa and MDA-MB-231 cancer cells. A TEM analysis confirmed the successful gold half-shell structure formation. High-performance liquid chromatography showed an encapsulation efficiency of the paclitaxel inside nanoparticles of more than 90%. In the release study, an initial burst release of about 20% in the first 24 h was observed, followed by a sustained drug release for a period as long as 10 days, reaching values of about 92% and 49% for NPs with and without near infrared laser irradiation. In in vitro cell internalization studies, targeted nanoparticles showed a higher accumulation than nontargeted nanoparticles, possibly through a specific interaction of the cyRGDfk with their homologous receptors, the ανβ3 y ανβ5 integrins on the cell surface. Compared with chemotherapy or photothermal treatment alone, the combined treatment demonstrated a synergistic effect, reducing the cell viability to 23% for the HeLa cells and 31% for the MDA-MB-231 cells. Thus, our results indicate that these multifuncional nanoparticles can be considered to be a promising targeted chemo-photothermal therapy system against cancer.https://www.mdpi.com/2072-666X/14/7/1390paclitaxelPLGAhalf shellcyRGDk peptidechemo-photothermal therapy |
| spellingShingle | Jaime Ibarra David Encinas-Basurto Mario Almada Josué Juárez Miguel Angel Valdez Silvia Barbosa Pablo Taboada Gold Half-Shell-Coated Paclitaxel-Loaded PLGA Nanoparticles for the Targeted Chemo-Photothermal Treatment of Cancer Micromachines paclitaxel PLGA half shell cyRGDk peptide chemo-photothermal therapy |
| title | Gold Half-Shell-Coated Paclitaxel-Loaded PLGA Nanoparticles for the Targeted Chemo-Photothermal Treatment of Cancer |
| title_full | Gold Half-Shell-Coated Paclitaxel-Loaded PLGA Nanoparticles for the Targeted Chemo-Photothermal Treatment of Cancer |
| title_fullStr | Gold Half-Shell-Coated Paclitaxel-Loaded PLGA Nanoparticles for the Targeted Chemo-Photothermal Treatment of Cancer |
| title_full_unstemmed | Gold Half-Shell-Coated Paclitaxel-Loaded PLGA Nanoparticles for the Targeted Chemo-Photothermal Treatment of Cancer |
| title_short | Gold Half-Shell-Coated Paclitaxel-Loaded PLGA Nanoparticles for the Targeted Chemo-Photothermal Treatment of Cancer |
| title_sort | gold half shell coated paclitaxel loaded plga nanoparticles for the targeted chemo photothermal treatment of cancer |
| topic | paclitaxel PLGA half shell cyRGDk peptide chemo-photothermal therapy |
| url | https://www.mdpi.com/2072-666X/14/7/1390 |
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