Promotion of cancer cell invasiveness and metastasis emergence caused by olfactory receptor stimulation.
Olfactory receptors (ORs) are expressed in the olfactory epithelium, where they detect odorants, but also in other tissues with additional functions. Some ORs are even overexpressed in tumor cells. In this study, we identified ORs expressed in enterochromaffin tumor cells by RT-PCR, showing that sin...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3885679?pdf=render |
| _version_ | 1819096180970225664 |
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| author | Guenhaël Sanz Isabelle Leray Aurélie Dewaele Julien Sobilo Stéphanie Lerondel Stéphan Bouet Denise Grébert Régine Monnerie Edith Pajot-Augy Lluis M Mir |
| author_facet | Guenhaël Sanz Isabelle Leray Aurélie Dewaele Julien Sobilo Stéphanie Lerondel Stéphan Bouet Denise Grébert Régine Monnerie Edith Pajot-Augy Lluis M Mir |
| author_sort | Guenhaël Sanz |
| collection | DOAJ |
| description | Olfactory receptors (ORs) are expressed in the olfactory epithelium, where they detect odorants, but also in other tissues with additional functions. Some ORs are even overexpressed in tumor cells. In this study, we identified ORs expressed in enterochromaffin tumor cells by RT-PCR, showing that single cells can co-express several ORs. Some of the receptors identified were already reported in other tumors, but they are orphan (without known ligand), as it is the case for most of the hundreds of human ORs. Thus, genes coding for human ORs with known ligands were transfected into these cells, expressing functional heterologous ORs. The in vitro stimulation of these cells by the corresponding OR odorant agonists promoted cell invasion of collagen gels. Using LNCaP prostate cancer cells, the stimulation of the PSGR (Prostate Specific G protein-coupled Receptor), an endogenously overexpressed OR, by β-ionone, its odorant agonist, resulted in the same phenotypic change. We also showed the involvement of a PI3 kinase γ dependent signaling pathway in this promotion of tumor cell invasiveness triggered by OR stimulation. Finally, after subcutaneous inoculation of LNCaP cells into NSG immunodeficient mice, the in vivo stimulation of these cells by the PSGR agonist β-ionone significantly enhanced metastasis emergence and spreading. |
| first_indexed | 2024-12-21T23:55:06Z |
| format | Article |
| id | doaj.art-0969df32526f4141b4f500a69303279e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T23:55:06Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0969df32526f4141b4f500a69303279e2022-12-21T18:45:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8511010.1371/journal.pone.0085110Promotion of cancer cell invasiveness and metastasis emergence caused by olfactory receptor stimulation.Guenhaël SanzIsabelle LerayAurélie DewaeleJulien SobiloStéphanie LerondelStéphan BouetDenise GrébertRégine MonnerieEdith Pajot-AugyLluis M MirOlfactory receptors (ORs) are expressed in the olfactory epithelium, where they detect odorants, but also in other tissues with additional functions. Some ORs are even overexpressed in tumor cells. In this study, we identified ORs expressed in enterochromaffin tumor cells by RT-PCR, showing that single cells can co-express several ORs. Some of the receptors identified were already reported in other tumors, but they are orphan (without known ligand), as it is the case for most of the hundreds of human ORs. Thus, genes coding for human ORs with known ligands were transfected into these cells, expressing functional heterologous ORs. The in vitro stimulation of these cells by the corresponding OR odorant agonists promoted cell invasion of collagen gels. Using LNCaP prostate cancer cells, the stimulation of the PSGR (Prostate Specific G protein-coupled Receptor), an endogenously overexpressed OR, by β-ionone, its odorant agonist, resulted in the same phenotypic change. We also showed the involvement of a PI3 kinase γ dependent signaling pathway in this promotion of tumor cell invasiveness triggered by OR stimulation. Finally, after subcutaneous inoculation of LNCaP cells into NSG immunodeficient mice, the in vivo stimulation of these cells by the PSGR agonist β-ionone significantly enhanced metastasis emergence and spreading.http://europepmc.org/articles/PMC3885679?pdf=render |
| spellingShingle | Guenhaël Sanz Isabelle Leray Aurélie Dewaele Julien Sobilo Stéphanie Lerondel Stéphan Bouet Denise Grébert Régine Monnerie Edith Pajot-Augy Lluis M Mir Promotion of cancer cell invasiveness and metastasis emergence caused by olfactory receptor stimulation. PLoS ONE |
| title | Promotion of cancer cell invasiveness and metastasis emergence caused by olfactory receptor stimulation. |
| title_full | Promotion of cancer cell invasiveness and metastasis emergence caused by olfactory receptor stimulation. |
| title_fullStr | Promotion of cancer cell invasiveness and metastasis emergence caused by olfactory receptor stimulation. |
| title_full_unstemmed | Promotion of cancer cell invasiveness and metastasis emergence caused by olfactory receptor stimulation. |
| title_short | Promotion of cancer cell invasiveness and metastasis emergence caused by olfactory receptor stimulation. |
| title_sort | promotion of cancer cell invasiveness and metastasis emergence caused by olfactory receptor stimulation |
| url | http://europepmc.org/articles/PMC3885679?pdf=render |
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