Stable multi-infection of splenocytes during SIV infection - the basis for continuous recombination
<p>Abstract</p> <p>Background</p> <p>Recombination is an important mechanism in the generation of genetic diversity of the human (HIV) and simian (SIV) immunodeficiency viruses. It requires the co-packaging of divergent RNA genomes into the same retroviral capsid and su...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-04-01
|
| Series: | Retrovirology |
| Subjects: | |
| Online Access: | http://www.retrovirology.com/content/9/1/31 |
| _version_ | 1819082530284896256 |
|---|---|
| author | Schultz Anke Sopper Sieghart Sauermann Ulrike Meyerhans Andreas Suspène Rodolphe |
| author_facet | Schultz Anke Sopper Sieghart Sauermann Ulrike Meyerhans Andreas Suspène Rodolphe |
| author_sort | Schultz Anke |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Recombination is an important mechanism in the generation of genetic diversity of the human (HIV) and simian (SIV) immunodeficiency viruses. It requires the co-packaging of divergent RNA genomes into the same retroviral capsid and subsequent template switching during the reverse transcription reaction. By HIV-specific fluorescence <it>in situ </it>hybridization (FISH), we have previously shown that the splenocytes from 2 chronically infected patients with Castelman's disease were multi-infected and thus fulfill the <it>in vivo </it>requirements to generate genetic diversity by recombination. In order to analyze when multi-infection first occurs during a lentivirus infection and how the distribution of multi-infection evolves during the disease course, we now determined the SIV copy numbers from splenocytes of 11 SIVmac251-infected rhesus macaques cross-sectionally covering the time span of primary infection throughout to end-stage immunodeficiency.</p> <p>Results</p> <p>SIV multi-infection of single splenocytes was readily detected in all monkeys and all stages of the infection. Single-infected cells were more frequent than double- or triple- infected cells. There was no strong trend linking the copy number distribution to plasma viral load, disease stage, or CD4 cell counts.</p> <p>Conclusions</p> <p>SIV multi-infection of single cells is already established during the primary infection phase thus enabling recombination to affect viral evolution <it>in vivo </it>throughout the disease course.</p> |
| first_indexed | 2024-12-21T20:18:08Z |
| format | Article |
| id | doaj.art-096dacb6491f4ccfb25fbc3465417225 |
| institution | Directory Open Access Journal |
| issn | 1742-4690 |
| language | English |
| last_indexed | 2024-12-21T20:18:08Z |
| publishDate | 2012-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Retrovirology |
| spelling | doaj.art-096dacb6491f4ccfb25fbc34654172252022-12-21T18:51:33ZengBMCRetrovirology1742-46902012-04-01913110.1186/1742-4690-9-31Stable multi-infection of splenocytes during SIV infection - the basis for continuous recombinationSchultz AnkeSopper SieghartSauermann UlrikeMeyerhans AndreasSuspène Rodolphe<p>Abstract</p> <p>Background</p> <p>Recombination is an important mechanism in the generation of genetic diversity of the human (HIV) and simian (SIV) immunodeficiency viruses. It requires the co-packaging of divergent RNA genomes into the same retroviral capsid and subsequent template switching during the reverse transcription reaction. By HIV-specific fluorescence <it>in situ </it>hybridization (FISH), we have previously shown that the splenocytes from 2 chronically infected patients with Castelman's disease were multi-infected and thus fulfill the <it>in vivo </it>requirements to generate genetic diversity by recombination. In order to analyze when multi-infection first occurs during a lentivirus infection and how the distribution of multi-infection evolves during the disease course, we now determined the SIV copy numbers from splenocytes of 11 SIVmac251-infected rhesus macaques cross-sectionally covering the time span of primary infection throughout to end-stage immunodeficiency.</p> <p>Results</p> <p>SIV multi-infection of single splenocytes was readily detected in all monkeys and all stages of the infection. Single-infected cells were more frequent than double- or triple- infected cells. There was no strong trend linking the copy number distribution to plasma viral load, disease stage, or CD4 cell counts.</p> <p>Conclusions</p> <p>SIV multi-infection of single cells is already established during the primary infection phase thus enabling recombination to affect viral evolution <it>in vivo </it>throughout the disease course.</p>http://www.retrovirology.com/content/9/1/31SIVFISHRecombinationProvirus copy number |
| spellingShingle | Schultz Anke Sopper Sieghart Sauermann Ulrike Meyerhans Andreas Suspène Rodolphe Stable multi-infection of splenocytes during SIV infection - the basis for continuous recombination Retrovirology SIV FISH Recombination Provirus copy number |
| title | Stable multi-infection of splenocytes during SIV infection - the basis for continuous recombination |
| title_full | Stable multi-infection of splenocytes during SIV infection - the basis for continuous recombination |
| title_fullStr | Stable multi-infection of splenocytes during SIV infection - the basis for continuous recombination |
| title_full_unstemmed | Stable multi-infection of splenocytes during SIV infection - the basis for continuous recombination |
| title_short | Stable multi-infection of splenocytes during SIV infection - the basis for continuous recombination |
| title_sort | stable multi infection of splenocytes during siv infection the basis for continuous recombination |
| topic | SIV FISH Recombination Provirus copy number |
| url | http://www.retrovirology.com/content/9/1/31 |
| work_keys_str_mv | AT schultzanke stablemultiinfectionofsplenocytesduringsivinfectionthebasisforcontinuousrecombination AT soppersieghart stablemultiinfectionofsplenocytesduringsivinfectionthebasisforcontinuousrecombination AT sauermannulrike stablemultiinfectionofsplenocytesduringsivinfectionthebasisforcontinuousrecombination AT meyerhansandreas stablemultiinfectionofsplenocytesduringsivinfectionthebasisforcontinuousrecombination AT suspenerodolphe stablemultiinfectionofsplenocytesduringsivinfectionthebasisforcontinuousrecombination |