Screening for Viruses in Indigenous Greek Black Pigs
The successful advancement of xenotransplantation has led to the development of highly sensitive detection systems for the screening of potentially zoonotic viruses in donor pigs and preventing their transmission to the recipient. To validate these methods, genetically modified pigs generated for xe...
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MDPI AG
2024-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/12/2/315 |
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| author | Hina Jhelum Vasileios Papatsiros Georgios Papakonstantinou Ludwig Krabben Benedikt Kaufer Joachim Denner |
| author_facet | Hina Jhelum Vasileios Papatsiros Georgios Papakonstantinou Ludwig Krabben Benedikt Kaufer Joachim Denner |
| author_sort | Hina Jhelum |
| collection | DOAJ |
| description | The successful advancement of xenotransplantation has led to the development of highly sensitive detection systems for the screening of potentially zoonotic viruses in donor pigs and preventing their transmission to the recipient. To validate these methods, genetically modified pigs generated for xenotransplantation, numerous minipigs and other pig breeds have been tested, thereby increasing our knowledge concerning the pig virome and the distribution of pig viruses. Of particular importance are the porcine cytomegalovirus, a porcine roseolovirus (PCMV/PRV) and the hepatitis E virus genotype 3 (HEV3). PCMV/PRV has been shown to reduce the survival time of pig transplants in non-human primates and was also transmitted in the first pig heart transplantation to a human patient. The main aim of this study was to determine the sensitivities of our methods to detect PCMV/PRV, HEV3, porcine lymphotropic herpesvirus-1 (PLHV-1), PLHV-2, PLHV-3, porcine circovirus 2 (PCV2), PCV3, PCV4 and porcine parvovirus 1 (PPV1) and to apply the methods to screen indigenous Greek black pigs. The high number of viruses found in these animals allowed for the evaluation of numerous detection methods. Since porcine endogenous retroviruses (PERVs) type A and B are integrated in the genome of all pigs, but PERV-C is not, the animals were screened for PERV-C and PERV-A/C. Our detection methods were sensitive and detected PCMV/PRV, PLHV-1, PLHV-1, PLHV-3, PVC3 and PERV-C in most animals. PPV1, HEV3, PCV4 and PERV-A/C were not detected. These data are of great interest since the animals are healthy and resistant to diseases. |
| first_indexed | 2024-03-07T22:20:29Z |
| format | Article |
| id | doaj.art-09795f74e2e845b7b6fb8953ed071303 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-07T22:20:29Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-09795f74e2e845b7b6fb8953ed0713032024-02-23T15:28:10ZengMDPI AGMicroorganisms2076-26072024-02-0112231510.3390/microorganisms12020315Screening for Viruses in Indigenous Greek Black PigsHina Jhelum0Vasileios Papatsiros1Georgios Papakonstantinou2Ludwig Krabben3Benedikt Kaufer4Joachim Denner5Institute of Virology, Free University Berlin, 14163 Berlin, GermanyFaculty of Veterinary Medicine, Clinic of Medicine (Farm Animal Medicine), University of Thessaly, 43100 Karditsa, GreeceFaculty of Veterinary Medicine, Clinic of Medicine (Farm Animal Medicine), University of Thessaly, 43100 Karditsa, GreeceInstitute of Virology, Free University Berlin, 14163 Berlin, GermanyInstitute of Virology, Free University Berlin, 14163 Berlin, GermanyInstitute of Virology, Free University Berlin, 14163 Berlin, GermanyThe successful advancement of xenotransplantation has led to the development of highly sensitive detection systems for the screening of potentially zoonotic viruses in donor pigs and preventing their transmission to the recipient. To validate these methods, genetically modified pigs generated for xenotransplantation, numerous minipigs and other pig breeds have been tested, thereby increasing our knowledge concerning the pig virome and the distribution of pig viruses. Of particular importance are the porcine cytomegalovirus, a porcine roseolovirus (PCMV/PRV) and the hepatitis E virus genotype 3 (HEV3). PCMV/PRV has been shown to reduce the survival time of pig transplants in non-human primates and was also transmitted in the first pig heart transplantation to a human patient. The main aim of this study was to determine the sensitivities of our methods to detect PCMV/PRV, HEV3, porcine lymphotropic herpesvirus-1 (PLHV-1), PLHV-2, PLHV-3, porcine circovirus 2 (PCV2), PCV3, PCV4 and porcine parvovirus 1 (PPV1) and to apply the methods to screen indigenous Greek black pigs. The high number of viruses found in these animals allowed for the evaluation of numerous detection methods. Since porcine endogenous retroviruses (PERVs) type A and B are integrated in the genome of all pigs, but PERV-C is not, the animals were screened for PERV-C and PERV-A/C. Our detection methods were sensitive and detected PCMV/PRV, PLHV-1, PLHV-1, PLHV-3, PVC3 and PERV-C in most animals. PPV1, HEV3, PCV4 and PERV-A/C were not detected. These data are of great interest since the animals are healthy and resistant to diseases.https://www.mdpi.com/2076-2607/12/2/315xenotransplantationvirus safetyporcine cytomegalovirus/porcine roseolovirus (PCMV/PRV)hepatitis E virusporcine endogenous retroviruses (PERVs) |
| spellingShingle | Hina Jhelum Vasileios Papatsiros Georgios Papakonstantinou Ludwig Krabben Benedikt Kaufer Joachim Denner Screening for Viruses in Indigenous Greek Black Pigs Microorganisms xenotransplantation virus safety porcine cytomegalovirus/porcine roseolovirus (PCMV/PRV) hepatitis E virus porcine endogenous retroviruses (PERVs) |
| title | Screening for Viruses in Indigenous Greek Black Pigs |
| title_full | Screening for Viruses in Indigenous Greek Black Pigs |
| title_fullStr | Screening for Viruses in Indigenous Greek Black Pigs |
| title_full_unstemmed | Screening for Viruses in Indigenous Greek Black Pigs |
| title_short | Screening for Viruses in Indigenous Greek Black Pigs |
| title_sort | screening for viruses in indigenous greek black pigs |
| topic | xenotransplantation virus safety porcine cytomegalovirus/porcine roseolovirus (PCMV/PRV) hepatitis E virus porcine endogenous retroviruses (PERVs) |
| url | https://www.mdpi.com/2076-2607/12/2/315 |
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