Successful Derivation of Hepatoblasts, Cholangiocytes and Hepatocytes from Simian Induced Pluripotent Stem Cells
The use of primary cells in human liver therapy is limited by a lack of cells. Induced pluripotent stem cells (iPSCs) represent an alternative to primary cells as they are infinitely expandable and can be differentiated into different liver cell types. The aim of our work was to demonstrate that sim...
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/18/10861 |
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| author | Eleanor Luce Clara Steichen Soumeya Abed Anne Weber Philippe Leboulch Leila Maouche-Chrétien Anne Dubart-Kupperschmitt |
| author_facet | Eleanor Luce Clara Steichen Soumeya Abed Anne Weber Philippe Leboulch Leila Maouche-Chrétien Anne Dubart-Kupperschmitt |
| author_sort | Eleanor Luce |
| collection | DOAJ |
| description | The use of primary cells in human liver therapy is limited by a lack of cells. Induced pluripotent stem cells (iPSCs) represent an alternative to primary cells as they are infinitely expandable and can be differentiated into different liver cell types. The aim of our work was to demonstrate that simian iPSCs (siPSCs) could be used as a new source of liver cells to be used as a large animal model for preclinical studies. We first differentiated siPSCs into a homogenous population of hepatoblasts (siHBs). We then separately differentiated them into hepatocytes (siHeps) and cholangiocytes (siChols) expressing respective specific markers and displaying epithelial polarity. Moreover, we showed that polarized siChols can self-organize into 3D structures. These results should facilitate the deciphering of liver development and open the way to exploring co-culture systems that could be assessed during preclinical studies, including in autologous monkey donors, for regenerative medicine purposes. |
| first_indexed | 2024-03-09T23:42:30Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:42:30Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0979766a90944985b5220e9f24868ba52023-11-23T16:49:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181086110.3390/ijms231810861Successful Derivation of Hepatoblasts, Cholangiocytes and Hepatocytes from Simian Induced Pluripotent Stem CellsEleanor Luce0Clara Steichen1Soumeya Abed2Anne Weber3Philippe Leboulch4Leila Maouche-Chrétien5Anne Dubart-Kupperschmitt6Unité Mixte de Recherche (UMR_S) 1193, INSERM/Université Paris-Saclay, F-94800 Villejuif, FranceUnité Mixte de Recherche (UMR_S) 1193, INSERM/Université Paris-Saclay, F-94800 Villejuif, FranceDivision of Innovative Therapies, Institute of Biology François Jacob, INSERM, Paris-Saclay University, CEA Fontenay aux Roses, F-92260 Fontenay-aux-Roses, FranceUnité Mixte de Recherche (UMR_S) 1193, INSERM/Université Paris-Saclay, F-94800 Villejuif, FranceDivision of Innovative Therapies, Institute of Biology François Jacob, INSERM, Paris-Saclay University, CEA Fontenay aux Roses, F-92260 Fontenay-aux-Roses, FranceDivision of Innovative Therapies, Institute of Biology François Jacob, INSERM, Paris-Saclay University, CEA Fontenay aux Roses, F-92260 Fontenay-aux-Roses, FranceUnité Mixte de Recherche (UMR_S) 1193, INSERM/Université Paris-Saclay, F-94800 Villejuif, FranceThe use of primary cells in human liver therapy is limited by a lack of cells. Induced pluripotent stem cells (iPSCs) represent an alternative to primary cells as they are infinitely expandable and can be differentiated into different liver cell types. The aim of our work was to demonstrate that simian iPSCs (siPSCs) could be used as a new source of liver cells to be used as a large animal model for preclinical studies. We first differentiated siPSCs into a homogenous population of hepatoblasts (siHBs). We then separately differentiated them into hepatocytes (siHeps) and cholangiocytes (siChols) expressing respective specific markers and displaying epithelial polarity. Moreover, we showed that polarized siChols can self-organize into 3D structures. These results should facilitate the deciphering of liver development and open the way to exploring co-culture systems that could be assessed during preclinical studies, including in autologous monkey donors, for regenerative medicine purposes.https://www.mdpi.com/1422-0067/23/18/10861simian induced pluripotent stem cellshepatocyte differentiationcholangiocyte differentiation |
| spellingShingle | Eleanor Luce Clara Steichen Soumeya Abed Anne Weber Philippe Leboulch Leila Maouche-Chrétien Anne Dubart-Kupperschmitt Successful Derivation of Hepatoblasts, Cholangiocytes and Hepatocytes from Simian Induced Pluripotent Stem Cells International Journal of Molecular Sciences simian induced pluripotent stem cells hepatocyte differentiation cholangiocyte differentiation |
| title | Successful Derivation of Hepatoblasts, Cholangiocytes and Hepatocytes from Simian Induced Pluripotent Stem Cells |
| title_full | Successful Derivation of Hepatoblasts, Cholangiocytes and Hepatocytes from Simian Induced Pluripotent Stem Cells |
| title_fullStr | Successful Derivation of Hepatoblasts, Cholangiocytes and Hepatocytes from Simian Induced Pluripotent Stem Cells |
| title_full_unstemmed | Successful Derivation of Hepatoblasts, Cholangiocytes and Hepatocytes from Simian Induced Pluripotent Stem Cells |
| title_short | Successful Derivation of Hepatoblasts, Cholangiocytes and Hepatocytes from Simian Induced Pluripotent Stem Cells |
| title_sort | successful derivation of hepatoblasts cholangiocytes and hepatocytes from simian induced pluripotent stem cells |
| topic | simian induced pluripotent stem cells hepatocyte differentiation cholangiocyte differentiation |
| url | https://www.mdpi.com/1422-0067/23/18/10861 |
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