Isolation and Characterization of Glycophorin from Carp Red Blood Cell Membranes
We isolated a high-purity carp glycophorin from carp erythrocyte membranes following extraction using the lithium diiodosalicylate (LIS)-phenol method and streptomycin treatment. The main carp glycophorin was observed to locate at the position of the carp and human band-3 proteins on an SDS-polyacry...
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-08-01
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| Series: | Membranes |
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| Online Access: | http://www.mdpi.com/2077-0375/4/3/491 |
| _version_ | 1797763537985077248 |
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| author | Takahiko Aoki Kenji Chimura Nobuhiro Nakao Yasuko Mizuno |
| author_facet | Takahiko Aoki Kenji Chimura Nobuhiro Nakao Yasuko Mizuno |
| author_sort | Takahiko Aoki |
| collection | DOAJ |
| description | We isolated a high-purity carp glycophorin from carp erythrocyte membranes following extraction using the lithium diiodosalicylate (LIS)-phenol method and streptomycin treatment. The main carp glycophorin was observed to locate at the position of the carp and human band-3 proteins on an SDS-polyacrylamide gel. Only the N-glycolylneuraminic acid (NeuGc) form of sialic acid was detected in the carp glycophorin. The oligosaccharide fraction was separated into two components (P-1 and P-2) using a Glyco-Pak DEAE column. We observed bacteriostatic activity against five strains of bacteria, including two known fish pathogens. Fractions from the carp erythrocyte membrane, the glycophorin oligosaccharide and the P-1 also exhibited bacteriostatic activity; whereas the glycolipid fraction and the glycophorin fraction without sialic acid did not show the activity. The carp glycophorin molecules attach to the flagellum of V. anguillarum or the cell surface of M. luteus and inhibited bacterial growth. |
| first_indexed | 2024-03-12T19:43:53Z |
| format | Article |
| id | doaj.art-0979b783a1df4c7a837f8b82bf033901 |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-12T19:43:53Z |
| publishDate | 2014-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-0979b783a1df4c7a837f8b82bf0339012023-08-02T03:38:13ZengMDPI AGMembranes2077-03752014-08-014349150810.3390/membranes4030491membranes4030491Isolation and Characterization of Glycophorin from Carp Red Blood Cell MembranesTakahiko Aoki0Kenji Chimura1Nobuhiro Nakao2Yasuko Mizuno3Laboratory of Quality in Marine Products, Graduate School of Bioresources, Mie University, 1577 Kurima Machiya-cho, Tsu, Mie 514-8507, JapanLaboratory of Quality in Marine Products, Graduate School of Bioresources, Mie University, 1577 Kurima Machiya-cho, Tsu, Mie 514-8507, JapanLaboratory of Quality in Marine Products, Graduate School of Bioresources, Mie University, 1577 Kurima Machiya-cho, Tsu, Mie 514-8507, JapanToray Research Center, Inc., 1111 Tebiro, Kamakura, Kanagawa 248-8555, JapanWe isolated a high-purity carp glycophorin from carp erythrocyte membranes following extraction using the lithium diiodosalicylate (LIS)-phenol method and streptomycin treatment. The main carp glycophorin was observed to locate at the position of the carp and human band-3 proteins on an SDS-polyacrylamide gel. Only the N-glycolylneuraminic acid (NeuGc) form of sialic acid was detected in the carp glycophorin. The oligosaccharide fraction was separated into two components (P-1 and P-2) using a Glyco-Pak DEAE column. We observed bacteriostatic activity against five strains of bacteria, including two known fish pathogens. Fractions from the carp erythrocyte membrane, the glycophorin oligosaccharide and the P-1 also exhibited bacteriostatic activity; whereas the glycolipid fraction and the glycophorin fraction without sialic acid did not show the activity. The carp glycophorin molecules attach to the flagellum of V. anguillarum or the cell surface of M. luteus and inhibited bacterial growth.http://www.mdpi.com/2077-0375/4/3/491fishred blood cell membraneserythrocyteglycophorinoligosaccharidesialic acidN-glycolylneuraminic acidbacteriostatic activity |
| spellingShingle | Takahiko Aoki Kenji Chimura Nobuhiro Nakao Yasuko Mizuno Isolation and Characterization of Glycophorin from Carp Red Blood Cell Membranes Membranes fish red blood cell membranes erythrocyte glycophorin oligosaccharide sialic acid N-glycolylneuraminic acid bacteriostatic activity |
| title | Isolation and Characterization of Glycophorin from Carp Red Blood Cell Membranes |
| title_full | Isolation and Characterization of Glycophorin from Carp Red Blood Cell Membranes |
| title_fullStr | Isolation and Characterization of Glycophorin from Carp Red Blood Cell Membranes |
| title_full_unstemmed | Isolation and Characterization of Glycophorin from Carp Red Blood Cell Membranes |
| title_short | Isolation and Characterization of Glycophorin from Carp Red Blood Cell Membranes |
| title_sort | isolation and characterization of glycophorin from carp red blood cell membranes |
| topic | fish red blood cell membranes erythrocyte glycophorin oligosaccharide sialic acid N-glycolylneuraminic acid bacteriostatic activity |
| url | http://www.mdpi.com/2077-0375/4/3/491 |
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