Overexpression of CsSAMT in Citrus sinensis Induces Defense Response and Increases Resistance to Xanthomonas citri subsp. citri
Citrus canker is a destructive disease caused by Xanthomonas citri subsp. citri, which affects all commercial sweet orange (Citrus sinensis [L.] Osbeck) cultivars. Salicylic acid (SA) and systemic-acquired resistance (SAR) have been demonstrated to have a crucial role in mediating plant defense resp...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.836582/full |
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| author | Cesar Augusto Nascimento Cesar Augusto Nascimento Natalia Sousa Teixeira-Silva Raquel Caserta Marcia Ortiz Mayo Marques Marco Aurelio Takita Alessandra A. de Souza |
| author_facet | Cesar Augusto Nascimento Cesar Augusto Nascimento Natalia Sousa Teixeira-Silva Raquel Caserta Marcia Ortiz Mayo Marques Marco Aurelio Takita Alessandra A. de Souza |
| author_sort | Cesar Augusto Nascimento |
| collection | DOAJ |
| description | Citrus canker is a destructive disease caused by Xanthomonas citri subsp. citri, which affects all commercial sweet orange (Citrus sinensis [L.] Osbeck) cultivars. Salicylic acid (SA) and systemic-acquired resistance (SAR) have been demonstrated to have a crucial role in mediating plant defense responses against this phytopathogen. To induce SAR, SA is converted to methyl salicylate (MeSA) by an SA-dependent methyltransferase (SAMT) and translocated systemically to prime noninfected distal tissues. Here, we generated sweet orange transgenic plants (based on cvs. Hamlin and Valencia) overexpressing the SAMT gene from Citrus (CsSAMT) and evaluated their resistance to citrus canker. We obtained four independent transgenic lines and confirmed their significantly higher MeSA volatilization compared to wild-type controls. Plants overexpressing CsSAMT showed reduced symptoms of citrus canker and bacterial populations in all transgenic lines without compromising plant development. One representative transgenic line (V44SAMT) was used to evaluate resistance response in primary and secondary sites. Without inoculation, V44SAMT modulated CsSAMT, CsNPR1, CsNPR3, and CsWRKY22 expression, indicating that this plant is in a primed defense status. The results demonstrate that MeSA signaling prompts the plant to respond more efficiently to pathogen attacks and induces immune responses in transgenic plants at both primary and secondary infection sites. |
| first_indexed | 2024-04-12T18:52:36Z |
| format | Article |
| id | doaj.art-0979bba9859b4b4f86f889bef6ce6f82 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T18:52:36Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-0979bba9859b4b4f86f889bef6ce6f822022-12-22T03:20:26ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-03-011310.3389/fpls.2022.836582836582Overexpression of CsSAMT in Citrus sinensis Induces Defense Response and Increases Resistance to Xanthomonas citri subsp. citriCesar Augusto Nascimento0Cesar Augusto Nascimento1Natalia Sousa Teixeira-Silva2Raquel Caserta3Marcia Ortiz Mayo Marques4Marco Aurelio Takita5Alessandra A. de Souza6Citrus Research Center “Sylvio Moreira”, Agronomic Institute – IAC, Cordeirópolis, BrazilDepartment of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas – UNICAMP, Campinas, BrazilCitrus Research Center “Sylvio Moreira”, Agronomic Institute – IAC, Cordeirópolis, BrazilCitrus Research Center “Sylvio Moreira”, Agronomic Institute – IAC, Cordeirópolis, BrazilDepartment of Phytochemistry, Agronomic Institute – IAC, Campinas, BrazilCitrus Research Center “Sylvio Moreira”, Agronomic Institute – IAC, Cordeirópolis, BrazilCitrus Research Center “Sylvio Moreira”, Agronomic Institute – IAC, Cordeirópolis, BrazilCitrus canker is a destructive disease caused by Xanthomonas citri subsp. citri, which affects all commercial sweet orange (Citrus sinensis [L.] Osbeck) cultivars. Salicylic acid (SA) and systemic-acquired resistance (SAR) have been demonstrated to have a crucial role in mediating plant defense responses against this phytopathogen. To induce SAR, SA is converted to methyl salicylate (MeSA) by an SA-dependent methyltransferase (SAMT) and translocated systemically to prime noninfected distal tissues. Here, we generated sweet orange transgenic plants (based on cvs. Hamlin and Valencia) overexpressing the SAMT gene from Citrus (CsSAMT) and evaluated their resistance to citrus canker. We obtained four independent transgenic lines and confirmed their significantly higher MeSA volatilization compared to wild-type controls. Plants overexpressing CsSAMT showed reduced symptoms of citrus canker and bacterial populations in all transgenic lines without compromising plant development. One representative transgenic line (V44SAMT) was used to evaluate resistance response in primary and secondary sites. Without inoculation, V44SAMT modulated CsSAMT, CsNPR1, CsNPR3, and CsWRKY22 expression, indicating that this plant is in a primed defense status. The results demonstrate that MeSA signaling prompts the plant to respond more efficiently to pathogen attacks and induces immune responses in transgenic plants at both primary and secondary infection sites.https://www.frontiersin.org/articles/10.3389/fpls.2022.836582/fullsystemic-acquired resistancesalicylic acidmethyl salicylateMeSAcitrus canker |
| spellingShingle | Cesar Augusto Nascimento Cesar Augusto Nascimento Natalia Sousa Teixeira-Silva Raquel Caserta Marcia Ortiz Mayo Marques Marco Aurelio Takita Alessandra A. de Souza Overexpression of CsSAMT in Citrus sinensis Induces Defense Response and Increases Resistance to Xanthomonas citri subsp. citri Frontiers in Plant Science systemic-acquired resistance salicylic acid methyl salicylate MeSA citrus canker |
| title | Overexpression of CsSAMT in Citrus sinensis Induces Defense Response and Increases Resistance to Xanthomonas citri subsp. citri |
| title_full | Overexpression of CsSAMT in Citrus sinensis Induces Defense Response and Increases Resistance to Xanthomonas citri subsp. citri |
| title_fullStr | Overexpression of CsSAMT in Citrus sinensis Induces Defense Response and Increases Resistance to Xanthomonas citri subsp. citri |
| title_full_unstemmed | Overexpression of CsSAMT in Citrus sinensis Induces Defense Response and Increases Resistance to Xanthomonas citri subsp. citri |
| title_short | Overexpression of CsSAMT in Citrus sinensis Induces Defense Response and Increases Resistance to Xanthomonas citri subsp. citri |
| title_sort | overexpression of cssamt in citrus sinensis induces defense response and increases resistance to xanthomonas citri subsp citri |
| topic | systemic-acquired resistance salicylic acid methyl salicylate MeSA citrus canker |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.836582/full |
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