Bioelectric stimulation controls tissue shape and size
Abstract Epithelial tissues sheath organs and electro-mechanically regulate ion and water transport to regulate development, homeostasis, and hydrostatic organ pressure. Here, we demonstrate how external electrical stimulation allows us to control these processes in living tissues. Specifically, we...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-47079-w |
| _version_ | 1797219641270992896 |
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| author | Gawoon Shim Isaac B. Breinyn Alejandro Martínez-Calvo Sameeksha Rao Daniel J. Cohen |
| author_facet | Gawoon Shim Isaac B. Breinyn Alejandro Martínez-Calvo Sameeksha Rao Daniel J. Cohen |
| author_sort | Gawoon Shim |
| collection | DOAJ |
| description | Abstract Epithelial tissues sheath organs and electro-mechanically regulate ion and water transport to regulate development, homeostasis, and hydrostatic organ pressure. Here, we demonstrate how external electrical stimulation allows us to control these processes in living tissues. Specifically, we electrically stimulate hollow, 3D kidneyoids and gut organoids and find that physiological-strength electrical stimulation of ∼ 5 - 10 V/cm powerfully inflates hollow tissues; a process we call electro-inflation. Electro-inflation is mediated by increased ion flux through ion channels/transporters and triggers subsequent osmotic water flow into the lumen, generating hydrostatic pressure that competes against cytoskeletal tension. Our computational studies suggest that electro-inflation is strongly driven by field-induced ion crowding on the outer surface of the tissue. Electrically stimulated tissues also break symmetry in 3D resulting from electrotaxis and affecting tissue shape. The ability of electrical cues to regulate tissue size and shape emphasizes the role and importance of the electrical micro-environment for living tissues. |
| first_indexed | 2024-04-24T12:36:52Z |
| format | Article |
| id | doaj.art-097b33eec8de4e00b8e55baf8f7814d6 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-24T12:36:52Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-097b33eec8de4e00b8e55baf8f7814d62024-04-07T11:24:29ZengNature PortfolioNature Communications2041-17232024-04-0115111710.1038/s41467-024-47079-wBioelectric stimulation controls tissue shape and sizeGawoon Shim0Isaac B. Breinyn1Alejandro Martínez-Calvo2Sameeksha Rao3Daniel J. Cohen4Department of Mechanical and Aerospace Engineering, Princeton UniversityDepartment of Quantitative and Computational Biology, Princeton UniversityPrinceton Center for Theoretical Science, Princeton UniversityDepartment of Mechanical and Aerospace Engineering, Princeton UniversityDepartment of Mechanical and Aerospace Engineering, Princeton UniversityAbstract Epithelial tissues sheath organs and electro-mechanically regulate ion and water transport to regulate development, homeostasis, and hydrostatic organ pressure. Here, we demonstrate how external electrical stimulation allows us to control these processes in living tissues. Specifically, we electrically stimulate hollow, 3D kidneyoids and gut organoids and find that physiological-strength electrical stimulation of ∼ 5 - 10 V/cm powerfully inflates hollow tissues; a process we call electro-inflation. Electro-inflation is mediated by increased ion flux through ion channels/transporters and triggers subsequent osmotic water flow into the lumen, generating hydrostatic pressure that competes against cytoskeletal tension. Our computational studies suggest that electro-inflation is strongly driven by field-induced ion crowding on the outer surface of the tissue. Electrically stimulated tissues also break symmetry in 3D resulting from electrotaxis and affecting tissue shape. The ability of electrical cues to regulate tissue size and shape emphasizes the role and importance of the electrical micro-environment for living tissues.https://doi.org/10.1038/s41467-024-47079-w |
| spellingShingle | Gawoon Shim Isaac B. Breinyn Alejandro Martínez-Calvo Sameeksha Rao Daniel J. Cohen Bioelectric stimulation controls tissue shape and size Nature Communications |
| title | Bioelectric stimulation controls tissue shape and size |
| title_full | Bioelectric stimulation controls tissue shape and size |
| title_fullStr | Bioelectric stimulation controls tissue shape and size |
| title_full_unstemmed | Bioelectric stimulation controls tissue shape and size |
| title_short | Bioelectric stimulation controls tissue shape and size |
| title_sort | bioelectric stimulation controls tissue shape and size |
| url | https://doi.org/10.1038/s41467-024-47079-w |
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