Detection of Chlamydia trachomatis in endocervical smears of sexually active women in Manaus-AM, Brazil, by PCR
Chlamydia trachomatis is now one of the most prevalent bacteria found in classic sexually transmissible diseases (STD), and as such, constitutes a serious public health problem. We examined the prevalence of Chlamydia trachomatis, by polymerase chain reaction (PCR), in 121 sexually active women who...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2003-01-01
|
| Series: | Brazilian Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000200001 |
| _version_ | 1824031299072950272 |
|---|---|
| author | Santos Cristina Teixeira Fabiane Vicente Ana Astolfi-Filho Spartaco |
| author_facet | Santos Cristina Teixeira Fabiane Vicente Ana Astolfi-Filho Spartaco |
| author_sort | Santos Cristina |
| collection | DOAJ |
| description | Chlamydia trachomatis is now one of the most prevalent bacteria found in classic sexually transmissible diseases (STD), and as such, constitutes a serious public health problem. We examined the prevalence of Chlamydia trachomatis, by polymerase chain reaction (PCR), in 121 sexually active women who sought treatment for STD in the Alfredo da Matta Institute of Dermatology and Venerology and the Institute of Tropical Medicine of Amazonas in Manaus, Brazil. These women were examined by a specific PCR for the chlamydial plasmid, and the nature of the amplicon was determined by restriction analysis and DNA sequencing. The PCR diagnosis revealed a prevalence of 20.7% infected women. |
| first_indexed | 2024-12-19T18:51:32Z |
| format | Article |
| id | doaj.art-097e917bdcb64431a3803786c8243a92 |
| institution | Directory Open Access Journal |
| issn | 1413-8670 1678-4391 |
| language | English |
| last_indexed | 2024-12-19T18:51:32Z |
| publishDate | 2003-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-097e917bdcb64431a3803786c8243a922022-12-21T20:09:51ZengElsevierBrazilian Journal of Infectious Diseases1413-86701678-43912003-01-01729195Detection of Chlamydia trachomatis in endocervical smears of sexually active women in Manaus-AM, Brazil, by PCRSantos CristinaTeixeira FabianeVicente AnaAstolfi-Filho SpartacoChlamydia trachomatis is now one of the most prevalent bacteria found in classic sexually transmissible diseases (STD), and as such, constitutes a serious public health problem. We examined the prevalence of Chlamydia trachomatis, by polymerase chain reaction (PCR), in 121 sexually active women who sought treatment for STD in the Alfredo da Matta Institute of Dermatology and Venerology and the Institute of Tropical Medicine of Amazonas in Manaus, Brazil. These women were examined by a specific PCR for the chlamydial plasmid, and the nature of the amplicon was determined by restriction analysis and DNA sequencing. The PCR diagnosis revealed a prevalence of 20.7% infected women.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000200001Chlamydia trachomatisPCRDST |
| spellingShingle | Santos Cristina Teixeira Fabiane Vicente Ana Astolfi-Filho Spartaco Detection of Chlamydia trachomatis in endocervical smears of sexually active women in Manaus-AM, Brazil, by PCR Brazilian Journal of Infectious Diseases Chlamydia trachomatis PCR DST |
| title | Detection of Chlamydia trachomatis in endocervical smears of sexually active women in Manaus-AM, Brazil, by PCR |
| title_full | Detection of Chlamydia trachomatis in endocervical smears of sexually active women in Manaus-AM, Brazil, by PCR |
| title_fullStr | Detection of Chlamydia trachomatis in endocervical smears of sexually active women in Manaus-AM, Brazil, by PCR |
| title_full_unstemmed | Detection of Chlamydia trachomatis in endocervical smears of sexually active women in Manaus-AM, Brazil, by PCR |
| title_short | Detection of Chlamydia trachomatis in endocervical smears of sexually active women in Manaus-AM, Brazil, by PCR |
| title_sort | detection of chlamydia trachomatis in endocervical smears of sexually active women in manaus am brazil by pcr |
| topic | Chlamydia trachomatis PCR DST |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000200001 |
| work_keys_str_mv | AT santoscristina detectionofchlamydiatrachomatisinendocervicalsmearsofsexuallyactivewomeninmanausambrazilbypcr AT teixeirafabiane detectionofchlamydiatrachomatisinendocervicalsmearsofsexuallyactivewomeninmanausambrazilbypcr AT vicenteana detectionofchlamydiatrachomatisinendocervicalsmearsofsexuallyactivewomeninmanausambrazilbypcr AT astolfifilhospartaco detectionofchlamydiatrachomatisinendocervicalsmearsofsexuallyactivewomeninmanausambrazilbypcr |