Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates
Summary: Neurorehabilitation strategies for ischemic stroke have shown promise for functional recovery, yet minimal tools are available to study rehabilitation techniques in non-human primates (NHPs). Here, we present a protocol to study rehabilitation techniques in NHPs using a photothrombotic tech...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672300463X |
| _version_ | 1797743963726151680 |
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| author | Noah Stanis Karam Khateeb Jasmine Zhou Ruikang K. Wang Azadeh Yazdan-Shahmorad |
| author_facet | Noah Stanis Karam Khateeb Jasmine Zhou Ruikang K. Wang Azadeh Yazdan-Shahmorad |
| author_sort | Noah Stanis |
| collection | DOAJ |
| description | Summary: Neurorehabilitation strategies for ischemic stroke have shown promise for functional recovery, yet minimal tools are available to study rehabilitation techniques in non-human primates (NHPs). Here, we present a protocol to study rehabilitation techniques in NHPs using a photothrombotic technique, a form of optical focal lesioning. We also describe steps for simultaneous neurophysiological recording and in vivo validation through vascular flow imaging. This interface can examine emerging neurorehabilitation strategies in the post-stroke environment in NHPs that are evolutionarily close to humans.For complete details on the use and execution of this protocol, please refer to Khateeb et al. (2022).6 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-12T15:03:04Z |
| format | Article |
| id | doaj.art-097ee43d1e624c4780e45f0897b049d9 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-12T15:03:04Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-097ee43d1e624c4780e45f0897b049d92023-08-13T04:54:43ZengElsevierSTAR Protocols2666-16672023-09-0143102496Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primatesNoah Stanis0Karam Khateeb1Jasmine Zhou2Ruikang K. Wang3Azadeh Yazdan-Shahmorad4Department of Bioengineering, University of Washington, Seattle, WA 98195, USA; Washington National Primate Research Center, Seattle, WA 98195, USADepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA; Washington National Primate Research Center, Seattle, WA 98195, USADepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA; Washington National Primate Research Center, Seattle, WA 98195, USADepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA; Department of Ophthalmology, University of Washington Medicine, Seattle, WA 98195, USADepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA; Washington National Primate Research Center, Seattle, WA 98195, USA; Department of Electrical and Computer Engineering, University of Washington, Seattle, WA 98195, USA; Corresponding authorSummary: Neurorehabilitation strategies for ischemic stroke have shown promise for functional recovery, yet minimal tools are available to study rehabilitation techniques in non-human primates (NHPs). Here, we present a protocol to study rehabilitation techniques in NHPs using a photothrombotic technique, a form of optical focal lesioning. We also describe steps for simultaneous neurophysiological recording and in vivo validation through vascular flow imaging. This interface can examine emerging neurorehabilitation strategies in the post-stroke environment in NHPs that are evolutionarily close to humans.For complete details on the use and execution of this protocol, please refer to Khateeb et al. (2022).6 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S266616672300463XBiotechnology and BioengineeringNeuroscience |
| spellingShingle | Noah Stanis Karam Khateeb Jasmine Zhou Ruikang K. Wang Azadeh Yazdan-Shahmorad Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates STAR Protocols Biotechnology and Bioengineering Neuroscience |
| title | Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates |
| title_full | Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates |
| title_fullStr | Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates |
| title_full_unstemmed | Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates |
| title_short | Protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non-human primates |
| title_sort | protocol to study ischemic stroke by photothrombotic lesioning in the cortex of non human primates |
| topic | Biotechnology and Bioengineering Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S266616672300463X |
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