Association of HSP70-2 Gene 1267A/G Polymorphism With Cataract Incidence Among Guilan Population
Background: Cataract is a visible opacity of the eye lens and it is the main reason of reversible blindness in the world. Oxidative stress is known as a major factor in cataract formation HSP70-2 protein is a molecular chaperone which is essential for cell survival in stress conditions. HSP70-2 gene...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Bushehr University of Medical Sciences
2017-01-01
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| Series: | Iranian South Medical Journal |
| Subjects: | |
| Online Access: | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-740&slc_lang=en&sid=1 |
| _version_ | 1811264556495798272 |
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| author | Zivar Salehi Zahra Gholaminia Mohammad Reza Panjtanpanah |
| author_facet | Zivar Salehi Zahra Gholaminia Mohammad Reza Panjtanpanah |
| author_sort | Zivar Salehi |
| collection | DOAJ |
| description | Background: Cataract is a visible opacity of the eye lens and it is the main reason of reversible blindness in the world. Oxidative stress is known as a major factor in cataract formation HSP70-2 protein is a molecular chaperone which is essential for cell survival in stress conditions. HSP70-2 gene is located in the human leukocyte antigen class ΙΙΙ region. This gene encodes an inducible protein. One of the common polymorphism of HSP70-2 is 1267A/G which is located in coding region. The aim of this study was to analysis of 1267A/G polymorphism of hsp70-2 gene in cataract patients.
Material and Methods: This case-control study included 118 cataract patients and 122 healthy people as a control groups. Genomic DNA was extracted from peripheral blood leukocytes and genotyping determination was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Statistical analysis was performed by using the MedCalc software (Version 12.1).
Results: The frequency of G allele was significantly higher in patients than the controls, (0.36 and 0.24, respectively). A higher frequency of the GG genotype of the HSP70-2 1267A/G polymorphism was found in the patients compared with controls (21.19% and 8.20%, respectively). The patients carrying the GG genotype were 3.2-fold at a higher risk of cataract compared with AA genotype (P=0.005).
Conclusion: The finding of this study suggested that the HSP70-2 1267A/G may affect the susceptibility to cataract in the studied population. Anyway the randomized multicenter studies with greater sample size still need to confirmed our results. |
| first_indexed | 2024-04-12T20:06:33Z |
| format | Article |
| id | doaj.art-0980119118c54abca47dfaa80ebdbcca |
| institution | Directory Open Access Journal |
| issn | 1735-4374 1735-6954 |
| language | English |
| last_indexed | 2024-04-12T20:06:33Z |
| publishDate | 2017-01-01 |
| publisher | Bushehr University of Medical Sciences |
| record_format | Article |
| series | Iranian South Medical Journal |
| spelling | doaj.art-0980119118c54abca47dfaa80ebdbcca2022-12-22T03:18:22ZengBushehr University of Medical SciencesIranian South Medical Journal1735-43741735-69542017-01-01196931939Association of HSP70-2 Gene 1267A/G Polymorphism With Cataract Incidence Among Guilan PopulationZivar Salehi0Zahra Gholaminia1Mohammad Reza Panjtanpanah2 Department of Biology, School of Sciences, University of Guilan, Rasht, Iran Department of Biology, School of Sciences, University of Guilan, Rasht, Iran Department of Ophthalmology, School of medicine, Guilan University of Medical Sciences, Rasht, Iran Background: Cataract is a visible opacity of the eye lens and it is the main reason of reversible blindness in the world. Oxidative stress is known as a major factor in cataract formation HSP70-2 protein is a molecular chaperone which is essential for cell survival in stress conditions. HSP70-2 gene is located in the human leukocyte antigen class ΙΙΙ region. This gene encodes an inducible protein. One of the common polymorphism of HSP70-2 is 1267A/G which is located in coding region. The aim of this study was to analysis of 1267A/G polymorphism of hsp70-2 gene in cataract patients. Material and Methods: This case-control study included 118 cataract patients and 122 healthy people as a control groups. Genomic DNA was extracted from peripheral blood leukocytes and genotyping determination was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Statistical analysis was performed by using the MedCalc software (Version 12.1). Results: The frequency of G allele was significantly higher in patients than the controls, (0.36 and 0.24, respectively). A higher frequency of the GG genotype of the HSP70-2 1267A/G polymorphism was found in the patients compared with controls (21.19% and 8.20%, respectively). The patients carrying the GG genotype were 3.2-fold at a higher risk of cataract compared with AA genotype (P=0.005). Conclusion: The finding of this study suggested that the HSP70-2 1267A/G may affect the susceptibility to cataract in the studied population. Anyway the randomized multicenter studies with greater sample size still need to confirmed our results.http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-740&slc_lang=en&sid=1Cataract gene polymorphism HSP70-2 gene oxidative stress |
| spellingShingle | Zivar Salehi Zahra Gholaminia Mohammad Reza Panjtanpanah Association of HSP70-2 Gene 1267A/G Polymorphism With Cataract Incidence Among Guilan Population Iranian South Medical Journal Cataract gene polymorphism HSP70-2 gene oxidative stress |
| title | Association of HSP70-2 Gene 1267A/G Polymorphism With Cataract Incidence Among Guilan Population |
| title_full | Association of HSP70-2 Gene 1267A/G Polymorphism With Cataract Incidence Among Guilan Population |
| title_fullStr | Association of HSP70-2 Gene 1267A/G Polymorphism With Cataract Incidence Among Guilan Population |
| title_full_unstemmed | Association of HSP70-2 Gene 1267A/G Polymorphism With Cataract Incidence Among Guilan Population |
| title_short | Association of HSP70-2 Gene 1267A/G Polymorphism With Cataract Incidence Among Guilan Population |
| title_sort | association of hsp70 2 gene 1267a g polymorphism with cataract incidence among guilan population |
| topic | Cataract gene polymorphism HSP70-2 gene oxidative stress |
| url | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-740&slc_lang=en&sid=1 |
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