Epidemiological surveillance of West Nile virus in the Eastern Plains of Colombia
Objective. Identify the presence of West Nile virus in horses and mosquitoes in eight municipalities of the Department of Meta. Materials and methods. The research was supported by the Bioethics Committee of the University of Los Llanos. 613 samples of Creole and quarter-mile equine horses, intended...
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| Format: | Article |
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Universidad de Cordoba
2020-01-01
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| Series: | Revista MVZ Cordoba |
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| Online Access: | https://revistamvz.unicordoba.edu.co/article/view/e1252/2380 |
| _version_ | 1818279904314130432 |
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| author | Diana P. Barajas P Karl A. Ciuoderis A Dario Cárdenas G Agustin Góngora O Jorge Osorio B Camilo E. Pacheco P Nestor I. Monroy O Gloria D Tovar B |
| author_facet | Diana P. Barajas P Karl A. Ciuoderis A Dario Cárdenas G Agustin Góngora O Jorge Osorio B Camilo E. Pacheco P Nestor I. Monroy O Gloria D Tovar B |
| author_sort | Diana P. Barajas P |
| collection | DOAJ |
| description | Objective. Identify the presence of West Nile virus in horses and mosquitoes in eight municipalities of the Department of Meta. Materials and methods. The research was supported by the Bioethics Committee of the University of Los Llanos. 613 samples of Creole and quarter-mile equine horses, intended for sports and work activities, with an age range of 2 to 15 years, were analyzed using serological and molecular tests in the transects: Villavicencio-Restrepo-Cumaral, Sanmartín- Castilla la Nueva-Granada and Puerto López-Puerto Gaitán, analyzed in 62 pools and 213 mosquitoes. The pool of sera of horses and mosquitoes were analyzed by ELISA and PCR. Results. No seropositive animals
were found by the ELISA test and molecular tests were also negative. Conclusions. Although in this study the presence of IgM antibodies was not evidenced by the Elisa technique, and molecular tests (RT-PCR) were also negative for viral circulation, in the municipalities under study, it is important to indicate that the molecular detection in sera, it requires representative levels of viremia and that the animal is in the acute phase of the disease. Although it is possible that the equine population
remains free of contact with the virus, epidemiological surveillance should be maintained against this important pathogen for human health, especially due to the outbreak of other zoonotic viruses such as Eastern Equine Encephalitis and Encephalitis Venezuelan Equine in the departments of Meta and Casanare, adjacent to this. |
| first_indexed | 2024-12-12T23:40:44Z |
| format | Article |
| id | doaj.art-0980dc15e5d2462ca73ad78a30ebdf10 |
| institution | Directory Open Access Journal |
| issn | 0122-0268 1909-0544 |
| language | English |
| last_indexed | 2024-12-12T23:40:44Z |
| publishDate | 2020-01-01 |
| publisher | Universidad de Cordoba |
| record_format | Article |
| series | Revista MVZ Cordoba |
| spelling | doaj.art-0980dc15e5d2462ca73ad78a30ebdf102022-12-22T00:07:09ZengUniversidad de CordobaRevista MVZ Cordoba0122-02681909-05442020-01-01251e1252https://doi.org/10.21897/rmvz.1252Epidemiological surveillance of West Nile virus in the Eastern Plains of ColombiaDiana P. Barajas P0https://orcid.org/0000-0002-7604-4734Karl A. Ciuoderis A1https://orcid.org/0000-0002-9267-3979Dario Cárdenas G2https://orcid.org/0000-0002-7364-3846Agustin Góngora O3https://orcid.org/0000-0002-2915-683XJorge Osorio B4https://orcid.org/0000-0002-7474-6150Camilo E. Pacheco P5https://orcid.org/0000-0002-3490-808XNestor I. Monroy O6https://orcid.org/0000-0002-3339-3862Gloria D Tovar B7https://orcid.org/0000-0002-7309-8104Universidad Cooperativa de Colombia, Facultad Medicina Veterinaria y Zootecnia, Grupo Los Araucos. Villavicencio, Meta, ColombiaUniversity of Wisconsin, Department of Pathobiological Sciences. Animal Health & Biomedical Sciences Building. Madison. Wisconsin. United StatesUniversidad Cooperativa de Colombia, Facultad Medicina Veterinaria y Zootecnia, Grupo Los Araucos. Villavicencio, Meta, Colombia.Universidad de los Llanos. Facultad Medicina Veterinaria y Zootecnia, Grupo GIRGA, Villavicencio, Meta, Colombia.University of Wisconsin, Department of Pathobiological Sciences. Animal Health & Biomedical Sciences Building. Madison. Wisconsin. United States 3Universidad de los Llanos.Universidad Cooperativa de Colombia, Facultad Medicina Veterinaria y Zootecnia, Grupo Los Araucos. Villavicencio, Meta, Colombia.Universidad Cooperativa de Colombia, Facultad Medicina Veterinaria y Zootecnia, Grupo Los Araucos. Villavicencio, Meta, Colombia.Insituto Colombiano Agropecuario. Villavicencio, Meta, Colombia.Objective. Identify the presence of West Nile virus in horses and mosquitoes in eight municipalities of the Department of Meta. Materials and methods. The research was supported by the Bioethics Committee of the University of Los Llanos. 613 samples of Creole and quarter-mile equine horses, intended for sports and work activities, with an age range of 2 to 15 years, were analyzed using serological and molecular tests in the transects: Villavicencio-Restrepo-Cumaral, Sanmartín- Castilla la Nueva-Granada and Puerto López-Puerto Gaitán, analyzed in 62 pools and 213 mosquitoes. The pool of sera of horses and mosquitoes were analyzed by ELISA and PCR. Results. No seropositive animals were found by the ELISA test and molecular tests were also negative. Conclusions. Although in this study the presence of IgM antibodies was not evidenced by the Elisa technique, and molecular tests (RT-PCR) were also negative for viral circulation, in the municipalities under study, it is important to indicate that the molecular detection in sera, it requires representative levels of viremia and that the animal is in the acute phase of the disease. Although it is possible that the equine population remains free of contact with the virus, epidemiological surveillance should be maintained against this important pathogen for human health, especially due to the outbreak of other zoonotic viruses such as Eastern Equine Encephalitis and Encephalitis Venezuelan Equine in the departments of Meta and Casanare, adjacent to this.https://revistamvz.unicordoba.edu.co/article/view/e1252/2380epidemiologyflaviviruszoonosespolymerase chain reaction |
| spellingShingle | Diana P. Barajas P Karl A. Ciuoderis A Dario Cárdenas G Agustin Góngora O Jorge Osorio B Camilo E. Pacheco P Nestor I. Monroy O Gloria D Tovar B Epidemiological surveillance of West Nile virus in the Eastern Plains of Colombia Revista MVZ Cordoba epidemiology flavivirus zoonoses polymerase chain reaction |
| title | Epidemiological surveillance of West Nile virus in the Eastern Plains of Colombia |
| title_full | Epidemiological surveillance of West Nile virus in the Eastern Plains of Colombia |
| title_fullStr | Epidemiological surveillance of West Nile virus in the Eastern Plains of Colombia |
| title_full_unstemmed | Epidemiological surveillance of West Nile virus in the Eastern Plains of Colombia |
| title_short | Epidemiological surveillance of West Nile virus in the Eastern Plains of Colombia |
| title_sort | epidemiological surveillance of west nile virus in the eastern plains of colombia |
| topic | epidemiology flavivirus zoonoses polymerase chain reaction |
| url | https://revistamvz.unicordoba.edu.co/article/view/e1252/2380 |
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