Evaluation of the Effect of Smoke of Anbar Nasara (Donkey Dung) Extract on Staphilococcus Aureus and Bacilus Subtilis
Background: The use of medicinal smokes in treatment of many diseases, including infectious microbial diseases, has long been popular in Iran. This study aimed to compare the antimicrobial effects of smoke of Anbar Nasara (donkey dung) extract on Staphylococcus aureus and Bacillus subtilis with comm...
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2017-01-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/7016 |
| _version_ | 1827879458951921664 |
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| author | Ardeshir Talebi Ebrahim Harigh Elnaz Dehdashtian Farzaneh Amini Marzieh Meshkat |
| author_facet | Ardeshir Talebi Ebrahim Harigh Elnaz Dehdashtian Farzaneh Amini Marzieh Meshkat |
| author_sort | Ardeshir Talebi |
| collection | DOAJ |
| description | Background: The use of medicinal smokes in treatment of many diseases, including infectious microbial diseases, has long been popular in Iran. This study aimed to compare the antimicrobial effects of smoke of Anbar Nasara (donkey dung) extract on Staphylococcus aureus and Bacillus subtilis with common antibiotics.
Methods: In this experimental study, donkey dung smoke as medicine and antibiotics as a control group were considered. Standard strains of Staphylococcus aureus and Bacillus subtilis were cultured in suitable mediums. Anbar Nasara extract was prepared without use of solvent, in order to prevent the antibacterial effect of solvent on microbes. Dilution and disk diffusion test was performed 3 times for each of the bacteria.
Findings: The mean of inhibition zone of Staphylococcus aureus by Anbar Nasara smoke was significantly greater than the inhibition zone induced by vancomycin; but there was no significant difference between the inhibition zone of Bacillus subtilis induced by Anbar Nasara smoke and ciprofloxacin. Least significant difference (LSD) post hoc test showed that the inhibition diameter of Staphylococcus aureus was significantly higher than the Bacillus subtilis. Bacillus subtilis induced higher minimum inhibitory concentration (MIC) values than Staphylococcus aureus; but, Bacillus subtilis induced higher minimum bactericidal concentration (MBC) values than Staphylococcus aureus.
Conclusion: The smoke of Anbar Nasara extract has a significant inhibitory effect on Staphylococcus aureus growth; so it can be offered as a medicine. |
| first_indexed | 2024-03-12T18:06:59Z |
| format | Article |
| id | doaj.art-0984d388b4f24ad48341dff8ff052326 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T18:06:59Z |
| publishDate | 2017-01-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-0984d388b4f24ad48341dff8ff0523262023-08-02T09:28:08ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2017-01-0134406135113552358Evaluation of the Effect of Smoke of Anbar Nasara (Donkey Dung) Extract on Staphilococcus Aureus and Bacilus SubtilisArdeshir Talebi0Ebrahim Harigh1Elnaz Dehdashtian2Farzaneh Amini3Marzieh Meshkat4Associate Professor, Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranIntern, Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Microbiology, School of Biological Sciences, Falavarjan Branch, Islamic Azad University, Isfahan, IranDepartment of Microbiology, School of Biological Sciences, Varamin- Pishva Branch, Islamic Azad University, Varamin, IranDepartment of Biology, Nourdanesh Institute of Higher Education, Meymeh, IranBackground: The use of medicinal smokes in treatment of many diseases, including infectious microbial diseases, has long been popular in Iran. This study aimed to compare the antimicrobial effects of smoke of Anbar Nasara (donkey dung) extract on Staphylococcus aureus and Bacillus subtilis with common antibiotics. Methods: In this experimental study, donkey dung smoke as medicine and antibiotics as a control group were considered. Standard strains of Staphylococcus aureus and Bacillus subtilis were cultured in suitable mediums. Anbar Nasara extract was prepared without use of solvent, in order to prevent the antibacterial effect of solvent on microbes. Dilution and disk diffusion test was performed 3 times for each of the bacteria. Findings: The mean of inhibition zone of Staphylococcus aureus by Anbar Nasara smoke was significantly greater than the inhibition zone induced by vancomycin; but there was no significant difference between the inhibition zone of Bacillus subtilis induced by Anbar Nasara smoke and ciprofloxacin. Least significant difference (LSD) post hoc test showed that the inhibition diameter of Staphylococcus aureus was significantly higher than the Bacillus subtilis. Bacillus subtilis induced higher minimum inhibitory concentration (MIC) values than Staphylococcus aureus; but, Bacillus subtilis induced higher minimum bactericidal concentration (MBC) values than Staphylococcus aureus. Conclusion: The smoke of Anbar Nasara extract has a significant inhibitory effect on Staphylococcus aureus growth; so it can be offered as a medicine.http://jims.mui.ac.ir/index.php/jims/article/view/7016Antimicrobial effectDonkey dungSmokeStaphylococcus aureusBacillus subtilis |
| spellingShingle | Ardeshir Talebi Ebrahim Harigh Elnaz Dehdashtian Farzaneh Amini Marzieh Meshkat Evaluation of the Effect of Smoke of Anbar Nasara (Donkey Dung) Extract on Staphilococcus Aureus and Bacilus Subtilis مجله دانشکده پزشکی اصفهان Antimicrobial effect Donkey dung Smoke Staphylococcus aureus Bacillus subtilis |
| title | Evaluation of the Effect of Smoke of Anbar Nasara (Donkey Dung) Extract on Staphilococcus Aureus and Bacilus Subtilis |
| title_full | Evaluation of the Effect of Smoke of Anbar Nasara (Donkey Dung) Extract on Staphilococcus Aureus and Bacilus Subtilis |
| title_fullStr | Evaluation of the Effect of Smoke of Anbar Nasara (Donkey Dung) Extract on Staphilococcus Aureus and Bacilus Subtilis |
| title_full_unstemmed | Evaluation of the Effect of Smoke of Anbar Nasara (Donkey Dung) Extract on Staphilococcus Aureus and Bacilus Subtilis |
| title_short | Evaluation of the Effect of Smoke of Anbar Nasara (Donkey Dung) Extract on Staphilococcus Aureus and Bacilus Subtilis |
| title_sort | evaluation of the effect of smoke of anbar nasara donkey dung extract on staphilococcus aureus and bacilus subtilis |
| topic | Antimicrobial effect Donkey dung Smoke Staphylococcus aureus Bacillus subtilis |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/7016 |
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