High-performance liquid chromatographic determination of β-carotene content in four varieties of lotus stamens
A high-performance liquid chromatography method was developed to determine -carotene content in lotus stamens.The stamens of four varieties of Nelumbo nucifera (Gaertn.) and ten samples bought from traditional drug stores were determined.Validation of the method was carried out, the linearity of th...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Prince of Songkla University
2010-08-01
|
| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://rdo.psu.ac.th/sjstweb/journal/32-4/0125-3395-32-4-373-377.pdf |
| _version_ | 1811196804858904576 |
|---|---|
| author | Nithida Phonkot Preeya Puangsomlee Wangsomnuk Chantana Aromdee |
| author_facet | Nithida Phonkot Preeya Puangsomlee Wangsomnuk Chantana Aromdee |
| author_sort | Nithida Phonkot |
| collection | DOAJ |
| description | A high-performance liquid chromatography method was developed to determine -carotene content in lotus stamens.The stamens of four varieties of Nelumbo nucifera (Gaertn.) and ten samples bought from traditional drug stores were determined.Validation of the method was carried out, the linearity of the -carotene concentrations range from 6.50-58.50 g.mL-1were 0.9997-0.9998. The repeatability and intermediate precision were 0.29 %CV (n=9) and 7.48 %CV (n=9), respectively; theaccuracy was 100.10 %w/w, the detection limit was 9.83 ng.mL-1 and the quantitation limit was 29.80 ng.mL-1. The -caroteneof the four varieties were 465.77-1150.80 mg% (n=3), and of the store samples were 4.70-41.73 mg% (n=3). The resultsdemonstrated that the contents varied according to the variety. The contents in the samples obtained from traditionaldrug stores were much lower that those dried in our laboratory, this might due to the source of the stamens, duration, andconditions of storages. |
| first_indexed | 2024-04-12T01:05:09Z |
| format | Article |
| id | doaj.art-0987c88a322246399e9ec6ca55b0d826 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-04-12T01:05:09Z |
| publishDate | 2010-08-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-0987c88a322246399e9ec6ca55b0d8262022-12-22T03:54:17ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952010-08-01324373377High-performance liquid chromatographic determination of β-carotene content in four varieties of lotus stamensNithida PhonkotPreeya Puangsomlee WangsomnukChantana AromdeeA high-performance liquid chromatography method was developed to determine -carotene content in lotus stamens.The stamens of four varieties of Nelumbo nucifera (Gaertn.) and ten samples bought from traditional drug stores were determined.Validation of the method was carried out, the linearity of the -carotene concentrations range from 6.50-58.50 g.mL-1were 0.9997-0.9998. The repeatability and intermediate precision were 0.29 %CV (n=9) and 7.48 %CV (n=9), respectively; theaccuracy was 100.10 %w/w, the detection limit was 9.83 ng.mL-1 and the quantitation limit was 29.80 ng.mL-1. The -caroteneof the four varieties were 465.77-1150.80 mg% (n=3), and of the store samples were 4.70-41.73 mg% (n=3). The resultsdemonstrated that the contents varied according to the variety. The contents in the samples obtained from traditionaldrug stores were much lower that those dried in our laboratory, this might due to the source of the stamens, duration, andconditions of storages.http://rdo.psu.ac.th/sjstweb/journal/32-4/0125-3395-32-4-373-377.pdfNelumbo nucifera (Gaertn.)carotenes in lotus stamensHPLC method |
| spellingShingle | Nithida Phonkot Preeya Puangsomlee Wangsomnuk Chantana Aromdee High-performance liquid chromatographic determination of β-carotene content in four varieties of lotus stamens Songklanakarin Journal of Science and Technology (SJST) Nelumbo nucifera (Gaertn.) carotenes in lotus stamens HPLC method |
| title | High-performance liquid chromatographic determination of β-carotene content in four varieties of lotus stamens |
| title_full | High-performance liquid chromatographic determination of β-carotene content in four varieties of lotus stamens |
| title_fullStr | High-performance liquid chromatographic determination of β-carotene content in four varieties of lotus stamens |
| title_full_unstemmed | High-performance liquid chromatographic determination of β-carotene content in four varieties of lotus stamens |
| title_short | High-performance liquid chromatographic determination of β-carotene content in four varieties of lotus stamens |
| title_sort | high performance liquid chromatographic determination of β carotene content in four varieties of lotus stamens |
| topic | Nelumbo nucifera (Gaertn.) carotenes in lotus stamens HPLC method |
| url | http://rdo.psu.ac.th/sjstweb/journal/32-4/0125-3395-32-4-373-377.pdf |
| work_keys_str_mv | AT nithidaphonkot highperformanceliquidchromatographicdeterminationofbcarotenecontentinfourvarietiesoflotusstamens AT preeyapuangsomleewangsomnuk highperformanceliquidchromatographicdeterminationofbcarotenecontentinfourvarietiesoflotusstamens AT chantanaaromdee highperformanceliquidchromatographicdeterminationofbcarotenecontentinfourvarietiesoflotusstamens |