Lupenone-Rich Fraction Derived from <i>Cissus quadrangularis</i> L. Suppresses Lipid Accumulation in 3T3-L1 Adipocytes
<i>Cissus quadrangularis</i> L. (CQ) has potential as a therapeutic for managing obesity and balancing metabolic activity, but the main bioactive compound and regulatory mechanism remain unknown. Herein, the CQ hexane extract was fractionated into 30 fractions (CQ-H) using flash column c...
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MDPI AG
2023-08-01
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| Series: | Life |
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| author | Thitiporn Lakthan Panupun Limpachayaporn Kanok-on Rayanil Pornsri Charoenpanich Pornwipa Phuangbubpha Adisri Charoenpanich |
| author_facet | Thitiporn Lakthan Panupun Limpachayaporn Kanok-on Rayanil Pornsri Charoenpanich Pornwipa Phuangbubpha Adisri Charoenpanich |
| author_sort | Thitiporn Lakthan |
| collection | DOAJ |
| description | <i>Cissus quadrangularis</i> L. (CQ) has potential as a therapeutic for managing obesity and balancing metabolic activity, but the main bioactive compound and regulatory mechanism remain unknown. Herein, the CQ hexane extract was fractionated into 30 fractions (CQ-H) using flash column chromatography and analyzed using thin-layer chromatography. The direct antiadipogenesis effect of CQ-H fractions was tested on 3T3-L1 preadipocytes. Lupenone-rich fractions 2H and 3H were identified as containing potent antiadipogenesis agents that reduced differentiated cell numbers and intracellular lipid droplet size. Although the overall mitochondrial density remained unchanged, differentiated cells exhibited a higher mitochondrial density than that in non-differentiated cells. Additionally, 2H increased mitochondrial activity in both cell types as shown by their differentiation and lipid formation stages. Lupenone was isolated from 2H (Lu-CQ) and shown to dose-dependently inhibit adipogenesis, with 2H being more potent than Lu-CQ. Lu-CQ and 2H downregulated the expression of <i>Pparg2</i> mRNA and upregulated that of glucose transporter genes, <i>Slc2a1</i> and <i>Slc2a4</i>. Lu-CQ and 2H induced increased glucose uptake by 3T3-L1 cells. These findings suggest that lupenone-rich fractions in CQ contribute to balancing metabolic activity and reducing adipose tissue formation. Further exploration of CQ and its components may prompt innovative strategies for managing obesity and metabolic disorders. |
| first_indexed | 2024-03-10T23:47:08Z |
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| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T23:47:08Z |
| publishDate | 2023-08-01 |
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| record_format | Article |
| series | Life |
| spelling | doaj.art-09886e1b41f946bcb964a9ed3a54d4702023-11-19T01:54:41ZengMDPI AGLife2075-17292023-08-01138172410.3390/life13081724Lupenone-Rich Fraction Derived from <i>Cissus quadrangularis</i> L. Suppresses Lipid Accumulation in 3T3-L1 AdipocytesThitiporn Lakthan0Panupun Limpachayaporn1Kanok-on Rayanil2Pornsri Charoenpanich3Pornwipa Phuangbubpha4Adisri Charoenpanich5Department of Biology, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, ThailandDepartment of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, ThailandDepartment of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, ThailandDepartment of Food Technology, Faculty of Engineering and Industrial Technology, Silpakorn University, Nakhon Pathom 73000, ThailandDepartment of Biology, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, ThailandDepartment of Biology, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand<i>Cissus quadrangularis</i> L. (CQ) has potential as a therapeutic for managing obesity and balancing metabolic activity, but the main bioactive compound and regulatory mechanism remain unknown. Herein, the CQ hexane extract was fractionated into 30 fractions (CQ-H) using flash column chromatography and analyzed using thin-layer chromatography. The direct antiadipogenesis effect of CQ-H fractions was tested on 3T3-L1 preadipocytes. Lupenone-rich fractions 2H and 3H were identified as containing potent antiadipogenesis agents that reduced differentiated cell numbers and intracellular lipid droplet size. Although the overall mitochondrial density remained unchanged, differentiated cells exhibited a higher mitochondrial density than that in non-differentiated cells. Additionally, 2H increased mitochondrial activity in both cell types as shown by their differentiation and lipid formation stages. Lupenone was isolated from 2H (Lu-CQ) and shown to dose-dependently inhibit adipogenesis, with 2H being more potent than Lu-CQ. Lu-CQ and 2H downregulated the expression of <i>Pparg2</i> mRNA and upregulated that of glucose transporter genes, <i>Slc2a1</i> and <i>Slc2a4</i>. Lu-CQ and 2H induced increased glucose uptake by 3T3-L1 cells. These findings suggest that lupenone-rich fractions in CQ contribute to balancing metabolic activity and reducing adipose tissue formation. Further exploration of CQ and its components may prompt innovative strategies for managing obesity and metabolic disorders.https://www.mdpi.com/2075-1729/13/8/1724<i>Cissus quadrangularis</i>3T3-L1antiadipogenesishexane extractlupenoneglucose uptake |
| spellingShingle | Thitiporn Lakthan Panupun Limpachayaporn Kanok-on Rayanil Pornsri Charoenpanich Pornwipa Phuangbubpha Adisri Charoenpanich Lupenone-Rich Fraction Derived from <i>Cissus quadrangularis</i> L. Suppresses Lipid Accumulation in 3T3-L1 Adipocytes Life <i>Cissus quadrangularis</i> 3T3-L1 antiadipogenesis hexane extract lupenone glucose uptake |
| title | Lupenone-Rich Fraction Derived from <i>Cissus quadrangularis</i> L. Suppresses Lipid Accumulation in 3T3-L1 Adipocytes |
| title_full | Lupenone-Rich Fraction Derived from <i>Cissus quadrangularis</i> L. Suppresses Lipid Accumulation in 3T3-L1 Adipocytes |
| title_fullStr | Lupenone-Rich Fraction Derived from <i>Cissus quadrangularis</i> L. Suppresses Lipid Accumulation in 3T3-L1 Adipocytes |
| title_full_unstemmed | Lupenone-Rich Fraction Derived from <i>Cissus quadrangularis</i> L. Suppresses Lipid Accumulation in 3T3-L1 Adipocytes |
| title_short | Lupenone-Rich Fraction Derived from <i>Cissus quadrangularis</i> L. Suppresses Lipid Accumulation in 3T3-L1 Adipocytes |
| title_sort | lupenone rich fraction derived from i cissus quadrangularis i l suppresses lipid accumulation in 3t3 l1 adipocytes |
| topic | <i>Cissus quadrangularis</i> 3T3-L1 antiadipogenesis hexane extract lupenone glucose uptake |
| url | https://www.mdpi.com/2075-1729/13/8/1724 |
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