Screen-printed carbon electrode/natural silica-ceria nanocomposite for electrochemical aptasensor application
A nanocomposite of natural silica and ceria was synthesized to modify a screen-printed carbon electrode (SPCE) to develop an aptasensor to detect epithelial sodium channel (ENaC) protein in urine as a biomarker of hypertension. The method steps were the synthesis of natural silica-ceria nanocomposi...
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| Format: | Article |
| Language: | English |
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International Association of Physical Chemists (IAPC)
2022-10-01
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| Series: | Journal of Electrochemical Science and Engineering |
| Subjects: | |
| Online Access: | http://pub.iapchem.org/ojs/index.php/JESE/article/view/1455 |
| _version_ | 1811228546030370816 |
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| author | Salma Nur Zakiyyah Diana Rakhmawaty Eddy M. Lutfi Firdaus Toto Subroto Yeni Wahyuni Hartati |
| author_facet | Salma Nur Zakiyyah Diana Rakhmawaty Eddy M. Lutfi Firdaus Toto Subroto Yeni Wahyuni Hartati |
| author_sort | Salma Nur Zakiyyah |
| collection | DOAJ |
| description |
A nanocomposite of natural silica and ceria was synthesized to modify a screen-printed carbon electrode (SPCE) to develop an aptasensor to detect epithelial sodium channel (ENaC) protein in urine as a biomarker of hypertension. The method steps were the synthesis of natural silica-ceria nanocomposite using the hydrothermal method, obtaining of natural silica nanoparticles from the extraction of alkaline silica sand and ceria nanoparticles from cerium nitrate, modification of SPCE/natural silica-ceria, immobilization of aptamer through streptavidin-biotin, and detection of ENaC protein concentration. Box-Behnken’s design was employed to determine the optimal conditions of aptamer concentration (0.5 μg mL-1), streptavidin incubation time (30 min), and aptamer incubation time (1 hour), respectively. Differential pulse voltammetry (DPV) characterization of the developed electrochemical aptasensor revealed that the [Fe(CN)6]3-/4- redox peak current increased from 3.190 to 9.073 μA, with detection and quantification limits of 0.113 and 0.343 ng mL-1, respectively. The method is proven as a simple and rapid method to monitor ENaC levels in urine samples.
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| first_indexed | 2024-04-12T09:59:20Z |
| format | Article |
| id | doaj.art-09899312bd714724a041c7285515f505 |
| institution | Directory Open Access Journal |
| issn | 1847-9286 |
| language | English |
| last_indexed | 2024-04-12T09:59:20Z |
| publishDate | 2022-10-01 |
| publisher | International Association of Physical Chemists (IAPC) |
| record_format | Article |
| series | Journal of Electrochemical Science and Engineering |
| spelling | doaj.art-09899312bd714724a041c7285515f5052022-12-22T03:37:36ZengInternational Association of Physical Chemists (IAPC)Journal of Electrochemical Science and Engineering1847-92862022-10-0110.5599/jese.1455Screen-printed carbon electrode/natural silica-ceria nanocomposite for electrochemical aptasensor applicationSalma Nur Zakiyyah0Diana Rakhmawaty Eddy1M. Lutfi Firdaus2Toto Subroto3Yeni Wahyuni Hartati4Department of Chemistry, Faculty of Mathematics and Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang km. 21 Jatinangor, Sumedang 45363, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang km. 21 Jatinangor, Sumedang 45363, IndonesiaGraduate School of Science Education, bengkulu University, Jl. W.R. Supratman Kandang Limun, Bengkulu 38371, IndonesiaBionformatics and Biomolecular Research Center, Universitas Padjadjaran, Jl. Singaperbangsa 2, Bandung 40132, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang km. 21 Jatinangor, Sumedang 45363, Indonesia A nanocomposite of natural silica and ceria was synthesized to modify a screen-printed carbon electrode (SPCE) to develop an aptasensor to detect epithelial sodium channel (ENaC) protein in urine as a biomarker of hypertension. The method steps were the synthesis of natural silica-ceria nanocomposite using the hydrothermal method, obtaining of natural silica nanoparticles from the extraction of alkaline silica sand and ceria nanoparticles from cerium nitrate, modification of SPCE/natural silica-ceria, immobilization of aptamer through streptavidin-biotin, and detection of ENaC protein concentration. Box-Behnken’s design was employed to determine the optimal conditions of aptamer concentration (0.5 μg mL-1), streptavidin incubation time (30 min), and aptamer incubation time (1 hour), respectively. Differential pulse voltammetry (DPV) characterization of the developed electrochemical aptasensor revealed that the [Fe(CN)6]3-/4- redox peak current increased from 3.190 to 9.073 μA, with detection and quantification limits of 0.113 and 0.343 ng mL-1, respectively. The method is proven as a simple and rapid method to monitor ENaC levels in urine samples. http://pub.iapchem.org/ojs/index.php/JESE/article/view/1455Silica-ceriasynthesis compositeaptamerENaC proteinhypertension biomarkervoltammetry |
| spellingShingle | Salma Nur Zakiyyah Diana Rakhmawaty Eddy M. Lutfi Firdaus Toto Subroto Yeni Wahyuni Hartati Screen-printed carbon electrode/natural silica-ceria nanocomposite for electrochemical aptasensor application Journal of Electrochemical Science and Engineering Silica-ceria synthesis composite aptamer ENaC protein hypertension biomarker voltammetry |
| title | Screen-printed carbon electrode/natural silica-ceria nanocomposite for electrochemical aptasensor application |
| title_full | Screen-printed carbon electrode/natural silica-ceria nanocomposite for electrochemical aptasensor application |
| title_fullStr | Screen-printed carbon electrode/natural silica-ceria nanocomposite for electrochemical aptasensor application |
| title_full_unstemmed | Screen-printed carbon electrode/natural silica-ceria nanocomposite for electrochemical aptasensor application |
| title_short | Screen-printed carbon electrode/natural silica-ceria nanocomposite for electrochemical aptasensor application |
| title_sort | screen printed carbon electrode natural silica ceria nanocomposite for electrochemical aptasensor application |
| topic | Silica-ceria synthesis composite aptamer ENaC protein hypertension biomarker voltammetry |
| url | http://pub.iapchem.org/ojs/index.php/JESE/article/view/1455 |
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