Risk factors associated with colistin resistance in carbapenemase-producing Enterobacterales: a multicenter study from a low-income country
Abstract Purpose The aim of this study was to assess the risk factors for colistin-resistant carbapenemase-producing Enterobacterales (CR-CPE), and describe the mortality associated with this organism, in a low-income country. Methods A descriptive, observational, and prospective multicenter study w...
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
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| Online Access: | https://doi.org/10.1186/s12941-023-00609-8 |
| _version_ | 1797752812376948736 |
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| author | Soria-Segarra Claudia Soria-Segarra Carmen Diaz Andrés Miranda-Ayala Marcela Cevallos-Apolo Kerly Bombón Moreno Bryan Chuzan J. John Gutierrez-Fernández José |
| author_facet | Soria-Segarra Claudia Soria-Segarra Carmen Diaz Andrés Miranda-Ayala Marcela Cevallos-Apolo Kerly Bombón Moreno Bryan Chuzan J. John Gutierrez-Fernández José |
| author_sort | Soria-Segarra Claudia |
| collection | DOAJ |
| description | Abstract Purpose The aim of this study was to assess the risk factors for colistin-resistant carbapenemase-producing Enterobacterales (CR-CPE), and describe the mortality associated with this organism, in a low-income country. Methods A descriptive, observational, and prospective multicenter study was carried out in Guayaquil, Ecuador. All patients with carbapenem-resistant Enterobacterales admitted between December 2021 and May 2022 were enrolled. Infection definitions were established according to the Centers for Disease Control and Prevention (CDC) protocols. The presence of carbapenemase-producing Enterobacterales was confirmed with a multiplex PCR for bla KPC, bla NDM, bla OXA-48, bla VIM, and bla IMP genes. MCR-1 production was studied molecularly, and MLST assays were carried out. Results Out of 114 patients enrolled in the study, 32 (28.07%) had at least one positive sample for CR-CPE. Klebsiella pneumoniae ST512-KPC-3 was the most frequent microorganism isolated. Parenteral feeding, β-lactamase inhibitor use, recent hemodialysis, and renal failure were all considered independent risk factors for carrying CR-CPE. A mortality of 41.22% was detected, but we could not find any difference between colistin-resistant and colistin-susceptible CPE. MCR-1 production was not detected in any of the isolates studied. Conclusion A significant burden for CR-CPE was found in a South American country that was mainly caused by the high-risk clone K. pneumoniae ST512-KPC-3 and not mediated by mcr-1 production. Its acquisition involved parenteral feeding, β-lactamase inhibitor use, recent hemodialysis, and renal failure as independent risk factors, demonstrating the critical need for infection prevention and stewardship programs to avoid dissemination to other countries in the region. |
| first_indexed | 2024-03-12T17:08:53Z |
| format | Article |
| id | doaj.art-09907130009441699a599ed6ec4bc2e8 |
| institution | Directory Open Access Journal |
| issn | 1476-0711 |
| language | English |
| last_indexed | 2024-03-12T17:08:53Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj.art-09907130009441699a599ed6ec4bc2e82023-08-06T11:09:18ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112023-08-012211910.1186/s12941-023-00609-8Risk factors associated with colistin resistance in carbapenemase-producing Enterobacterales: a multicenter study from a low-income countrySoria-Segarra Claudia0Soria-Segarra Carmen1Diaz Andrés2Miranda-Ayala Marcela3Cevallos-Apolo Kerly4Bombón Moreno Bryan5Chuzan J. John6Gutierrez-Fernández José7Sosecali. Medical ServicesSosecali. Medical ServicesHospital GuayaquilOmni HospitalHospital de Infectología Dr. José Daniel Rodríguez MaridueñaDepartment of Human Biology, University of TorontoDepartment of Microbiology, Hospital AlcívarDepartment of Microbiology, Hospital Virgen de las Nieves, Institute for Biosanitary Research-IbsAbstract Purpose The aim of this study was to assess the risk factors for colistin-resistant carbapenemase-producing Enterobacterales (CR-CPE), and describe the mortality associated with this organism, in a low-income country. Methods A descriptive, observational, and prospective multicenter study was carried out in Guayaquil, Ecuador. All patients with carbapenem-resistant Enterobacterales admitted between December 2021 and May 2022 were enrolled. Infection definitions were established according to the Centers for Disease Control and Prevention (CDC) protocols. The presence of carbapenemase-producing Enterobacterales was confirmed with a multiplex PCR for bla KPC, bla NDM, bla OXA-48, bla VIM, and bla IMP genes. MCR-1 production was studied molecularly, and MLST assays were carried out. Results Out of 114 patients enrolled in the study, 32 (28.07%) had at least one positive sample for CR-CPE. Klebsiella pneumoniae ST512-KPC-3 was the most frequent microorganism isolated. Parenteral feeding, β-lactamase inhibitor use, recent hemodialysis, and renal failure were all considered independent risk factors for carrying CR-CPE. A mortality of 41.22% was detected, but we could not find any difference between colistin-resistant and colistin-susceptible CPE. MCR-1 production was not detected in any of the isolates studied. Conclusion A significant burden for CR-CPE was found in a South American country that was mainly caused by the high-risk clone K. pneumoniae ST512-KPC-3 and not mediated by mcr-1 production. Its acquisition involved parenteral feeding, β-lactamase inhibitor use, recent hemodialysis, and renal failure as independent risk factors, demonstrating the critical need for infection prevention and stewardship programs to avoid dissemination to other countries in the region.https://doi.org/10.1186/s12941-023-00609-8ColistinResistanceCarbapenemase-producingEnterobacteralesRisk factors |
| spellingShingle | Soria-Segarra Claudia Soria-Segarra Carmen Diaz Andrés Miranda-Ayala Marcela Cevallos-Apolo Kerly Bombón Moreno Bryan Chuzan J. John Gutierrez-Fernández José Risk factors associated with colistin resistance in carbapenemase-producing Enterobacterales: a multicenter study from a low-income country Annals of Clinical Microbiology and Antimicrobials Colistin Resistance Carbapenemase-producing Enterobacterales Risk factors |
| title | Risk factors associated with colistin resistance in carbapenemase-producing Enterobacterales: a multicenter study from a low-income country |
| title_full | Risk factors associated with colistin resistance in carbapenemase-producing Enterobacterales: a multicenter study from a low-income country |
| title_fullStr | Risk factors associated with colistin resistance in carbapenemase-producing Enterobacterales: a multicenter study from a low-income country |
| title_full_unstemmed | Risk factors associated with colistin resistance in carbapenemase-producing Enterobacterales: a multicenter study from a low-income country |
| title_short | Risk factors associated with colistin resistance in carbapenemase-producing Enterobacterales: a multicenter study from a low-income country |
| title_sort | risk factors associated with colistin resistance in carbapenemase producing enterobacterales a multicenter study from a low income country |
| topic | Colistin Resistance Carbapenemase-producing Enterobacterales Risk factors |
| url | https://doi.org/10.1186/s12941-023-00609-8 |
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